公开(公告)号：US20050176036A1

公开(公告)日：2005-08-11

申请号：US11008275

申请日：2004-12-10

申请人： Takahide Yokoi ,  Takashi Minowa ,  Toshiro Saito ,  Yoshiko Kaku

发明人： Takahide Yokoi ,  Takashi Minowa ,  Toshiro Saito ,  Yoshiko Kaku

IPC分类号： G01N33/53 ,  C12M1/00 ,  C12M1/34 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N37/00 ,  B05D3/00

CPC分类号： C12Q1/6837 ,  C12Q2600/158 ,  C12Q2525/179

摘要： Methods for global monitoring of gene expression and searching for useful genes that are targeted to organisms lacking sequence information are realized by providing low-cost and efficient DNA arrays. A genomic library constructed from randomly cleaved genomic DNA fragments is directly fixed on a substrate that allows the library to be individually recognized. In this way, global monitoring of gene expression can be carried out without being limited by the amount of gene sequence information. Further, plasmids that are detected by a constructed random genomic DNA array are fragmented into shorter DNAs, which are fixed on another substrate to construct a sub DNA array. With the use of this sub DNA array, gene expression is analyzed and useful genes are searched.

摘要翻译： 通过提供低成本和有效的DNA阵列来实现全球监测基因表达和寻找靶向缺乏序列信息的生物体的有用基因的方法。 由随机切割的基因组DNA片段构建的基因组文库直接固定在允许文库单独识别的底物上。 以这种方式，可以在不受基因序列信息量的限制的情况下进行基因表达的全局监测。 此外，通过构建的随机基因组DNA阵列检测的质粒被片段化成较短的DNA，其被固定在另一底物上以构建亚DNA阵列。 通过使用该DNA DNA阵列，分析基因表达，并搜索有用的基因。