公开(公告)号：US20140073054A1

公开(公告)日：2014-03-13

申请号：US13610015

申请日：2012-09-11

申请人： KUANG-TSE HUANG ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

发明人： KUANG-TSE HUANG ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

IPC分类号： C12N15/81

CPC分类号： C12N15/81 ,  C07K14/395 ,  C12P7/06 ,  Y02E50/17

摘要： The invention provides a yeast strain and a method for making the same. The method has the step of replacing the regulation region upstream of the hsp104 gene in the genome of the yeast, so as to accelerate and prolong the expression span of hsp104 gene and enhance the capability of the yeast to ferment and produce ethanol in a high-temperature environment. The yeast is capable of fermenting glucose at a temperature higher than 42° C. to produce ethanol, or biomass ethanol, wherein the ethanol production ratio based on fermentation of glucose is higher than 97%. Being able to synchronize the degradation/hydrolysis stage and fermentation stage of biomass ethanol producing process, the yeast in accordance with the present invention is able to lower the production cost of biomass ethanol and further raise the productivity with its high ethanol production ratio.

摘要翻译： 本发明提供酵母菌株及其制备方法。 该方法具有代替酵母基因组中hsp104基因上游调节区的步骤，加速并延长了hsp104基因的表达量，提高了酵母发酵和生产乙醇的能力， 温度环境。 酵母能够在高于42℃的温度下发酵葡萄糖以生产乙醇或生物质乙醇，其中基于葡萄糖发酵的乙醇生产比高于97％。 能够使生物质乙醇生产工艺的降解/水解阶段和发酵阶段同步，根据本发明的酵母能够降低生物质乙醇的生产成本，并且以高乙醇生产比进一步提高生产率。

公开(公告)号：US08663970B1

公开(公告)日：2014-03-04

申请号：US13610015

申请日：2012-09-11

申请人： Kuang-Tse Huang ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

发明人： Kuang-Tse Huang ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

IPC分类号： C12N1/00 ,  C12N15/74

CPC分类号： C12N15/81 ,  C07K14/395 ,  C12P7/06 ,  Y02E50/17

摘要： The invention provides a yeast strain and a method for making the same. The method has the step of replacing the regulation region upstream of the hsp104 gene in the genome of the yeast, so as to accelerate and prolong the expression span of hsp104 gene and enhance the capability of the yeast to ferment and produce ethanol in a high-temperature environment. The yeast is capable of fermenting glucose at a temperature higher than 42° C. to produce ethanol, or biomass ethanol, wherein the ethanol production ratio based on fermentation of glucose is higher than 97%. Being able to synchronize the degradation/hydrolysis stage and fermentation stage of biomass ethanol producing process, the yeast in accordance with the present invention is able to lower the production cost of biomass ethanol and further raise the productivity with its high ethanol production ratio.

摘要翻译： 本发明提供酵母菌株及其制备方法。 该方法具有代替酵母基因组中hsp104基因上游调节区的步骤，加速并延长了hsp104基因的表达量，提高了酵母发酵和生产乙醇的能力， 温度环境。 酵母能够在高于42℃的温度下发酵葡萄糖以生产乙醇或生物质乙醇，其中基于葡萄糖发酵的乙醇生产比高于97％。 能够使生物质乙醇生产工艺的降解/水解阶段和发酵阶段同步，根据本发明的酵母能够降低生物质乙醇的生产成本，并且以高乙醇生产比进一步提高生产率。