公开(公告)号：US08663970B1

公开(公告)日：2014-03-04

申请号：US13610015

申请日：2012-09-11

申请人： Kuang-Tse Huang ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

发明人： Kuang-Tse Huang ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

IPC分类号： C12N1/00 ,  C12N15/74

CPC分类号： C12N15/81 ,  C07K14/395 ,  C12P7/06 ,  Y02E50/17

摘要： The invention provides a yeast strain and a method for making the same. The method has the step of replacing the regulation region upstream of the hsp104 gene in the genome of the yeast, so as to accelerate and prolong the expression span of hsp104 gene and enhance the capability of the yeast to ferment and produce ethanol in a high-temperature environment. The yeast is capable of fermenting glucose at a temperature higher than 42° C. to produce ethanol, or biomass ethanol, wherein the ethanol production ratio based on fermentation of glucose is higher than 97%. Being able to synchronize the degradation/hydrolysis stage and fermentation stage of biomass ethanol producing process, the yeast in accordance with the present invention is able to lower the production cost of biomass ethanol and further raise the productivity with its high ethanol production ratio.

摘要翻译： 本发明提供酵母菌株及其制备方法。 该方法具有代替酵母基因组中hsp104基因上游调节区的步骤，加速并延长了hsp104基因的表达量，提高了酵母发酵和生产乙醇的能力， 温度环境。 酵母能够在高于42℃的温度下发酵葡萄糖以生产乙醇或生物质乙醇，其中基于葡萄糖发酵的乙醇生产比高于97％。 能够使生物质乙醇生产工艺的降解/水解阶段和发酵阶段同步，根据本发明的酵母能够降低生物质乙醇的生产成本，并且以高乙醇生产比进一步提高生产率。

公开(公告)号：US08669316B2

公开(公告)日：2014-03-11

申请号：US13482356

申请日：2012-05-29

申请人： Wen-Chien Lee ,  Yu-Zong Lin ,  Yu-Sheng Lin ,  Tzu-Hsien Wang

发明人： Wen-Chien Lee ,  Yu-Zong Lin ,  Yu-Sheng Lin ,  Tzu-Hsien Wang

IPC分类号： C12N1/20 ,  H01F1/00 ,  H01F1/26 ,  C07H21/00 ,  C07H21/04 ,  C08K3/22

CPC分类号： C07K1/18 ,  B22F2999/00 ,  B82Y30/00 ,  H01F1/0063 ,  B22F1/0018 ,  C22C2202/02

摘要： Magnetic ion-exchange polymer microspheres and a method for preparing the same are provided. The method for preparing the magnetic ion-exchange polymer microspheres includes swelling the ion-exchange resins and allowing the magnetic nano-particles to enter the interior of the ion-exchange resins. The magnetic ion-exchange resins of the present invention have various functional groups can be introduced onto the surfaces thereof. Therefore, the magnetic ion-exchange resins of the present invention can be applied in many areas, and thereby they have high economic value.

摘要翻译： 提供了磁性离子交换聚合物微球及其制备方法。 制备磁性离子交换聚合物微球的方法包括使离子交换树脂溶胀并使磁性纳米颗粒进入离子交换树脂的内部。 本发明的磁性离子交换树脂可以将各种官能团引入其表面。 因此，本发明的磁性离子交换树脂可以应用于许多领域，因此具有较高的经济价值。

公开(公告)号：US20130149772A1

公开(公告)日：2013-06-13

申请号：US13482356

申请日：2012-05-29

申请人： Wen-Chien Lee ,  Yu-Zong Lin ,  Yu-Sheng Lin ,  Tzu-Hsien Wang

发明人： Wen-Chien Lee ,  Yu-Zong Lin ,  Yu-Sheng Lin ,  Tzu-Hsien Wang

IPC分类号： H01F1/01 ,  C07H21/02 ,  C07H21/04 ,  C07K16/00 ,  C07K1/18 ,  C12N1/20 ,  C07H1/06 ,  C07K14/00 ,  B82Y30/00 ,  B82Y40/00

CPC分类号： C07K1/18 ,  B22F2999/00 ,  B82Y30/00 ,  H01F1/0063 ,  B22F1/0018 ,  C22C2202/02

摘要： Magnetic ion-exchange polymer microspheres and a method for preparing the same are provided. The method for preparing the magnetic ion-exchange polymer microspheres includes swelling the ion-exchange resins and allowing the magnetic nano-particles to enter the interior of the ion-exchange resins. The magnetic ion-exchange resins of the present invention have various functional groups can be introduced onto the surfaces thereof. Therefore, the magnetic ion-exchange resins of the present invention can be applied in many areas, and thereby they have high economic value.

摘要翻译： 提供了磁性离子交换聚合物微球及其制备方法。 制备磁性离子交换聚合物微球的方法包括使离子交换树脂溶胀并使磁性纳米颗粒进入离子交换树脂的内部。 本发明的磁性离子交换树脂可以将各种官能团引入其表面。 因此，本发明的磁性离子交换树脂可以应用于许多领域，因此具有较高的经济价值。

公开(公告)号：US06379922B1

公开(公告)日：2002-04-30

申请号：US09665719

申请日：2000-09-20

申请人： Wen-Chien Lee

发明人： Wen-Chien Lee

IPC分类号： C12P100

CPC分类号： C12N11/02 ,  C12M21/18 ,  C12M23/38 ,  C12M25/14 ,  C12M29/04 ,  C12P37/06

摘要： An enzyme membrane reactor is designed to enhance the production efficiency of foods, pharmaceutical products, and other bioproducts. The reactor is formed of a cap and a container in which a plurality of the biocatalyst-immobilized sheets are located at an interval. The reactor works in such a way that a liquid reaction mixture is introduced into the container via an inlet of the container, thereby resulting in the synthesis of a bioproduct mixture which is collected via an outlet of the reactor. The immobilized biocatalyst is prepared by entrapping a biocatalyst with a gluten matrix. The immobilized biocatalyst is deposited on supporting meshes contained by a plurality of frames that are inserted in a plurality of pairs of grooves contained by two opposite side walls of the container. Deactivated biocatalyst-immobilized sheets are replaceable and recyclable. The biocatalyst-immobilized sheets of the reactor have a low trans-membrane pressure and a low intra-membrane diffusion resistance against the liquid reaction mixture, thereby resulting in an efficient production of bioproducts.

摘要翻译： 设计了酶膜反应器，以提高食品，药品和其他生物产品的生产效率。 反应器由盖和容器形成，其中多个生物催化剂固定的片材以一定间隔设置。 反应器的工作方式是通过容器的入口将液体反应混合物引入容器中，从而导致通过反应器的出口收集的生物产物混合物的合成。 固定化的生物催化剂是通过将生物催化剂与谷蛋白基质截留来制备的。 固定化的生物催化剂沉积在由多个框架所包含的支撑网上，所述多个框架被插入由容器的两个相对的侧壁容纳的多对沟槽中。 去活化的生物催化剂固定的片材是可替代的和可回收的。 反应器的生物催化剂固定的片材对液体反应混合物具有低的跨膜压力和低的膜内扩散阻力，从而有效地生产生物产物。

公开(公告)号：US20060130192A1

公开(公告)日：2006-06-15

申请号：US11299800

申请日：2005-12-13

申请人： Wen-Chien Lee ,  Ting-Hao Chung

发明人： Wen-Chien Lee ,  Ting-Hao Chung

IPC分类号： H01F1/04

CPC分类号： B82Y25/00 ,  B82Y10/00 ,  H01F1/0063

摘要： Magnetic polymer microbeads and a method for preparing the same are provided. The method for preparing the magnetic polymer microbeads includes the following steps: preparing polymer particles; immersing the polymer particles into a solution in order to swell the polymer particles; adding magnetic nanoparticles to the solution and allowing the magnetic nanoparticlse to enter an interior of the polymer particles; and separating the polymer particles from the solution, wherein the polymer particle is made of polystyrene, or a copolymer containing styrene, and the solution includes a medium polar solvent. The average particle size of the magnetic polymer microbeads of the present invention ranges from submicrons to microns. The magnetic polymer microbeads of the present invention have high magnetization, and the various functional groups can be introduced onto the surfaces thereof. Therefore, the magnetic polymer microbeads of the present invention can be applied in many areas, and thereby they have high economic value.

摘要翻译： 提供了磁性聚合物微珠及其制备方法。 制备磁性聚合物微珠的方法包括以下步骤：制备聚合物颗粒; 将聚合物颗粒浸入溶液中以使聚合物颗粒溶胀; 向溶液中加入磁性纳米颗粒并使磁性纳米颗粒进入聚合物颗粒的内部; 并从溶液中分离聚合物颗粒，其中聚合物颗粒由聚苯乙烯制成，或含有苯乙烯的共聚物，该溶液包括中等极性溶剂。 本发明的磁性聚合物微珠的平均粒径为亚微米至微米。 本发明的磁性聚合物微珠具有高的磁化强度，并且可以将各种官能团引入其表面。 因此，本发明的磁性聚合物微珠可以应用于许多领域，因此具有较高的经济价值。

公开(公告)号：US20140073054A1

公开(公告)日：2014-03-13

申请号：US13610015

申请日：2012-09-11

申请人： KUANG-TSE HUANG ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

发明人： KUANG-TSE HUANG ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

IPC分类号： C12N15/81

CPC分类号： C12N15/81 ,  C07K14/395 ,  C12P7/06 ,  Y02E50/17

摘要： The invention provides a yeast strain and a method for making the same. The method has the step of replacing the regulation region upstream of the hsp104 gene in the genome of the yeast, so as to accelerate and prolong the expression span of hsp104 gene and enhance the capability of the yeast to ferment and produce ethanol in a high-temperature environment. The yeast is capable of fermenting glucose at a temperature higher than 42° C. to produce ethanol, or biomass ethanol, wherein the ethanol production ratio based on fermentation of glucose is higher than 97%. Being able to synchronize the degradation/hydrolysis stage and fermentation stage of biomass ethanol producing process, the yeast in accordance with the present invention is able to lower the production cost of biomass ethanol and further raise the productivity with its high ethanol production ratio.

摘要翻译： 本发明提供酵母菌株及其制备方法。 该方法具有代替酵母基因组中hsp104基因上游调节区的步骤，加速并延长了hsp104基因的表达量，提高了酵母发酵和生产乙醇的能力， 温度环境。 酵母能够在高于42℃的温度下发酵葡萄糖以生产乙醇或生物质乙醇，其中基于葡萄糖发酵的乙醇生产比高于97％。 能够使生物质乙醇生产工艺的降解/水解阶段和发酵阶段同步，根据本发明的酵母能够降低生物质乙醇的生产成本，并且以高乙醇生产比进一步提高生产率。

公开(公告)号：US07754278B2

公开(公告)日：2010-07-13

申请号：US11299800

申请日：2005-12-13

申请人： Wen-Chien Lee ,  Ting-Hao Chung

发明人： Wen-Chien Lee ,  Ting-Hao Chung

IPC分类号： B05D7/00

CPC分类号： B82Y25/00 ,  B82Y10/00 ,  H01F1/0063

摘要： Magnetic polymer microbeads and a method for preparing the same are provided. The method for preparing the magnetic polymer microbeads includes the following steps: preparing polymer particles; immersing the polymer particles into a solution in order to swell the polymer particles; adding magnetic nanoparticles to the solution and allowing the magnetic nanoparticles to enter an interior of the polymer particles; and separating the polymer particles from the solution, wherein the polymer particle is made of polystyrene, or a copolymer containing styrene, and the solution includes a medium polar solvent. The average particle size of the magnetic polymer microbeads of the present invention ranges from submicrons to microns. The magnetic polymer microbeads of the present invention have high magnetization, and the various functional groups can be introduced onto the surfaces thereof. Therefore, the magnetic polymer microbeads of the present invention can be applied in many areas, and thereby they have high economic value.

摘要翻译： 提供了磁性聚合物微珠及其制备方法。 制备磁性聚合物微珠的方法包括以下步骤：制备聚合物颗粒; 将聚合物颗粒浸入溶液中以使聚合物颗粒溶胀; 向溶液中加入磁性纳米颗粒并使磁性纳米颗粒进入聚合物颗粒的内部; 并从溶液中分离聚合物颗粒，其中聚合物颗粒由聚苯乙烯制成，或含有苯乙烯的共聚物，该溶液包括中等极性溶剂。 本发明的磁性聚合物微珠的平均粒径为亚微米至微米。 本发明的磁性聚合物微珠具有高的磁化强度，并且可以将各种官能团引入其表面。 因此，本发明的磁性聚合物微珠可以应用于许多领域，因此具有较高的经济价值。