公开(公告)号：US08663970B1

公开(公告)日：2014-03-04

申请号：US13610015

申请日：2012-09-11

申请人： Kuang-Tse Huang ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

发明人： Kuang-Tse Huang ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

IPC分类号： C12N1/00 ,  C12N15/74

CPC分类号： C12N15/81 ,  C07K14/395 ,  C12P7/06 ,  Y02E50/17

摘要： The invention provides a yeast strain and a method for making the same. The method has the step of replacing the regulation region upstream of the hsp104 gene in the genome of the yeast, so as to accelerate and prolong the expression span of hsp104 gene and enhance the capability of the yeast to ferment and produce ethanol in a high-temperature environment. The yeast is capable of fermenting glucose at a temperature higher than 42° C. to produce ethanol, or biomass ethanol, wherein the ethanol production ratio based on fermentation of glucose is higher than 97%. Being able to synchronize the degradation/hydrolysis stage and fermentation stage of biomass ethanol producing process, the yeast in accordance with the present invention is able to lower the production cost of biomass ethanol and further raise the productivity with its high ethanol production ratio.

摘要翻译： 本发明提供酵母菌株及其制备方法。 该方法具有代替酵母基因组中hsp104基因上游调节区的步骤，加速并延长了hsp104基因的表达量，提高了酵母发酵和生产乙醇的能力， 温度环境。 酵母能够在高于42℃的温度下发酵葡萄糖以生产乙醇或生物质乙醇，其中基于葡萄糖发酵的乙醇生产比高于97％。 能够使生物质乙醇生产工艺的降解/水解阶段和发酵阶段同步，根据本发明的酵母能够降低生物质乙醇的生产成本，并且以高乙醇生产比进一步提高生产率。

公开(公告)号：US20110054010A1

公开(公告)日：2011-03-03

申请号：US12626969

申请日：2009-11-30

申请人： Kuang-Tse HUANG ,  Jian-En LIN

发明人： Kuang-Tse HUANG ,  Jian-En LIN

IPC分类号： A61K31/7052 ,  C07H21/00 ,  A61P43/00

CPC分类号： A61K31/7052 ,  A61K47/42 ,  C12N15/1135 ,  C12N2310/13

摘要： A pharmaceutical composition having a transcription factor decoy and a pharmaceutical acceptable carrier thereof is provided, wherein the transcription factor decoy is TCF decoy. A method for curing or preventing endotoxin-treated endothelial cells from apoptosis is provided. A method for treating or meliorating septic shock is also provided. The transcription factor decoy is effective on melioration of endothelial cell death after lipopolysaccharide challenge. Furthermore, the pharmaceutical composition is easy to be prepared, economic and has low immunogenicity.

摘要翻译： 提供具有转录因子诱饵及其药学上可接受的载体的药物组合物，其中转录因子诱饵是TCF诱饵。 提供了一种治疗或预​​防内毒素处理的内皮细胞凋亡的方法。 还提供了治疗或改善败血性休克的方法。 转录因子诱饵对脂多糖攻击后内皮细胞死亡的改善有效。 此外，药物组合物易于制备，经济且免疫原性低。

公开(公告)号：US07795401B2

公开(公告)日：2010-09-14

申请号：US11515863

申请日：2006-09-06

申请人： Kuang-Tse Huang ,  Yen-Lin Lin

发明人： Kuang-Tse Huang ,  Yen-Lin Lin

IPC分类号： A61K35/14

CPC分类号： C07K14/805

摘要： The present invention relates generally to a modified hemoglobin with an allosteric effector conjugated which is capable of dynamically regulating the efficiency of oxygen release. The hemoglobin composition provided in the present invention is utilizing maleimide-Polyethylene glycol-N-hydroxysuccinimidyl as a linker to crossbridge the peptide comprising SEQ ID NO: 1 to the surface of a hemoglobin, and hence, it is able to decrease the oxygen affinity through the characteristic of the peptide which could enter into the central cavity formed between β- chains of hemoglobin under an appropriate oxygen concentration.

摘要翻译： 本发明一般涉及能够动态调节氧释放效率的具有变构​​效应子共轭的改性血红蛋白。 本发明中提供的血红蛋白组合物是利用马来酰亚胺 - 聚乙二醇-N-羟基琥珀酰亚胺基作为接头将包含SEQ ID NO：1的肽交叉到血红蛋白的表面，因此能够通过 在适当的氧气浓度下，能够进入血红蛋白的链之间形成的中心腔的肽的特征。

公开(公告)号：US20140073054A1

公开(公告)日：2014-03-13

申请号：US13610015

申请日：2012-09-11

申请人： KUANG-TSE HUANG ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

发明人： KUANG-TSE HUANG ,  Hau-Ren Chen ,  Wen-Chien Lee ,  Chang-Yu Wu ,  Hsin-Cheng Chen ,  Yu-Long Wu ,  Meng-Tsu Tsai ,  Ju-Ping Yeh ,  Yu-Wei Liang ,  Yu-Shiuan Lai

IPC分类号： C12N15/81

CPC分类号： C12N15/81 ,  C07K14/395 ,  C12P7/06 ,  Y02E50/17

摘要： The invention provides a yeast strain and a method for making the same. The method has the step of replacing the regulation region upstream of the hsp104 gene in the genome of the yeast, so as to accelerate and prolong the expression span of hsp104 gene and enhance the capability of the yeast to ferment and produce ethanol in a high-temperature environment. The yeast is capable of fermenting glucose at a temperature higher than 42° C. to produce ethanol, or biomass ethanol, wherein the ethanol production ratio based on fermentation of glucose is higher than 97%. Being able to synchronize the degradation/hydrolysis stage and fermentation stage of biomass ethanol producing process, the yeast in accordance with the present invention is able to lower the production cost of biomass ethanol and further raise the productivity with its high ethanol production ratio.

摘要翻译： 本发明提供酵母菌株及其制备方法。 该方法具有代替酵母基因组中hsp104基因上游调节区的步骤，加速并延长了hsp104基因的表达量，提高了酵母发酵和生产乙醇的能力， 温度环境。 酵母能够在高于42℃的温度下发酵葡萄糖以生产乙醇或生物质乙醇，其中基于葡萄糖发酵的乙醇生产比高于97％。 能够使生物质乙醇生产工艺的降解/水解阶段和发酵阶段同步，根据本发明的酵母能够降低生物质乙醇的生产成本，并且以高乙醇生产比进一步提高生产率。

公开(公告)号：US08153607B2

公开(公告)日：2012-04-10

申请号：US12626969

申请日：2009-11-30

申请人： Kuang-Tse Huang ,  Jian-En Lin

发明人： Kuang-Tse Huang ,  Jian-En Lin

IPC分类号： A61K31/70 ,  C07H21/04 ,  C12Q1/00

CPC分类号： A61K31/7052 ,  A61K47/42 ,  C12N15/1135 ,  C12N2310/13

摘要： A pharmaceutical composition having a transcription factor decoy and a pharmaceutical acceptable carrier thereof is provided, wherein the transcription factor decoy is TCF decoy. A method for curing or preventing endotoxin-treated endothelial cells from apoptosis is provided. A method for treating or meliorating septic shock is also provided. The transcription factor decoy is effective on melioration of endothelial cell death after lipopolysaccharide challenge. Furthermore, the pharmaceutical composition is easy to be prepared, economic and has low immunogenicity.

摘要翻译： 提供具有转录因子诱饵及其药学上可接受的载体的药物组合物，其中转录因子诱饵是TCF诱饵。 提供了一种治疗或预​​防内毒素处理的内皮细胞凋亡的方法。 还提供了治疗或改善败血性休克的方法。 转录因子诱饵对脂多糖攻击后内皮细胞死亡的改善有效。 此外，药物组合物易于制备，经济且免疫原性低。

公开(公告)号：US20080064855A1

公开(公告)日：2008-03-13

申请号：US11515863

申请日：2006-09-06

申请人： Kuang-Tse Huang ,  Yen-Lin Lin

发明人： Kuang-Tse Huang ,  Yen-Lin Lin

IPC分类号： C07K14/805

CPC分类号： C07K14/805

摘要： The present invention relates generally to a modified hemoglobin with an allosteric effector conjugated which is capable of dynamically regulating the efficiency of oxygen release. The hemoglobin composition provided in the present invention is utilizing maleimide-Polyethylene glycol-N-hydroxysuccinimidyl as a linker to crossbridge the peptide comprising SEQ ID NO: 1 to the surface of a hemoglobin, and hence, it would able to modulate the oxygen affinity through the characteristic of the peptide which could enter into the central cavity formed between β- chains of hemoglobin under an appropriate oxygen concentration.

摘要翻译： 本发明一般涉及能够动态调节氧释放效率的具有变构​​效应子共轭的改性血红蛋白。 本发明中提供的血红蛋白组合物是利用马来酰亚胺 - 聚乙二醇-N-羟基琥珀酰亚胺基作为连接体将包含SEQ ID NO：1的肽交叉到血红蛋白的表面，因此，它能够通过 可能进入中心腔的肽的特征是形成于“img id =”CUSTOM-CHARACTER-00001“he =”2.79mm“wi =”1.78mm“file =”US20080064855A1-20080313-P00001.TIF“ 在合适的氧浓度下，血红蛋白链的img-content =“character”img-format =“tif”/>链。