公开(公告)号：US20110117617A1

公开(公告)日：2011-05-19

申请号：US12620142

申请日：2009-11-17

申请人： Dehua LIU ,  Hongjuan LIU ,  Xianjin OU ,  Yan Sun ,  Yunzhen Xu ,  Yueyong LEI ,  Weibin LIU

发明人： Dehua LIU ,  Hongjuan LIU ,  Xianjin OU ,  Yan Sun ,  Yunzhen Xu ,  Yueyong LEI ,  Weibin LIU

IPC分类号： C12P7/42 ,  C12N1/21 ,  C12P7/18

CPC分类号： C12P7/16 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/625 ,  Y02E50/10

摘要： The present invention relates to methods and genetically engineered micro-organisms for the combined production of PDO, BDO, and PHP by fermentation. The micro-organism is characterized in that its D-lactate dehydrogenase gene has been deleted or functionally inactivated, and it comprises heterogenous polynucleotides encoding the Coenzyme A-dependent Aldehyde dehydrogenase and the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms are also disclosed

摘要翻译： 本发明涉及通过发酵联合生产PDO，BDO和PHP的方法和遗传工程微生物。 微生物的特征在于其D-乳酸脱氢酶基因已被缺失或功能失活，并且其包含编码辅酶A依赖性醛脱氢酶和聚羟基链烷酸酯合酶的异源多核苷酸。 还公开了构建这种微生物的方法

公开(公告)号：US08507250B2

公开(公告)日：2013-08-13

申请号：US13527933

申请日：2012-06-20

申请人： Dehua Liu ,  Hongjuan Liu ,  Xianjin Ou ,  Yan Sun ,  Yunzhen Xu ,  Yueyong Lei ,  Weibin Liu

发明人： Dehua Liu ,  Hongjuan Liu ,  Xianjin Ou ,  Yan Sun ,  Yunzhen Xu ,  Yueyong Lei ,  Weibin Liu

IPC分类号： C12P7/42 ,  C12P7/18 ,  C12N9/00 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12P7/16 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/625 ,  Y02E50/10

摘要： The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.

摘要翻译： 本发明涉及通过发酵联合生产1,3-丙二醇（PDO），2,3-丁二醇（BDO）和多羟基丙酸（PHP）的遗传工程微生物。 特别地，本发明涉及适用于通过发酵联合生产PDO，BDO和PHP的遗传工程微生物，其特征在于：与相应的野生型起始微生物相比，遗传上的D-乳酸脱氢酶基因 遗传工程微生物包含编码辅酶A依赖性醛脱氢酶的异源多核苷酸和编码聚羟基链烷酸酯合酶的异源多核苷酸。 还教导了构建这种微生物的方法，以及通过遗传工程化细菌的发酵联合生产PDO，BDO和PHP的方法。

公开(公告)号：US20120258521A1

公开(公告)日：2012-10-11

申请号：US13527933

申请日：2012-06-20

申请人： Dehua LIU ,  Hongjuan LIU ,  Xianjin OU ,  Yan SUN ,  Yunzhen XU ,  Yueyong LEI ,  Weibin LIU

发明人： Dehua LIU ,  Hongjuan LIU ,  Xianjin OU ,  Yan SUN ,  Yunzhen XU ,  Yueyong LEI ,  Weibin LIU

IPC分类号： C12N1/21

CPC分类号： C12P7/16 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/625 ,  Y02E50/10

摘要： The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.

摘要翻译： 本发明涉及通过发酵联合生产1,3-丙二醇（PDO），2,3-丁二醇（BDO）和多羟基丙酸（PHP）的遗传工程微生物。 特别地，本发明涉及适用于通过发酵联合生产PDO，BDO和PHP的遗传工程微生物，其特征在于：与相应的野生型起始微生物相比，遗传上的D-乳酸脱氢酶基因 遗传工程微生物包含编码辅酶A依赖性醛脱氢酶的异源多核苷酸和编码聚羟基链烷酸酯合酶的异源多核苷酸。 还教导了构建这种微生物的方法，以及通过遗传工程化细菌的发酵联合生产PDO，BDO和PHP的方法。

公开(公告)号：US08227217B2

公开(公告)日：2012-07-24

申请号：US12620142

申请日：2009-11-17

申请人： Dehua Liu ,  Hongjuan Liu ,  Xianjin Ou ,  Yan Sun ,  Yunzhen Xu ,  Yueyong Lei ,  Weibin Liu

发明人： Dehua Liu ,  Hongjuan Liu ,  Xianjin Ou ,  Yan Sun ,  Yunzhen Xu ,  Yueyong Lei ,  Weibin Liu

IPC分类号： C12P7/42 ,  C12P7/18 ,  C12N9/00 ,  C12N15/00

CPC分类号： C12P7/16 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/625 ,  Y02E50/10

摘要： The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.

摘要翻译： 本发明涉及通过发酵联合生产1,3-丙二醇（PDO），2,3-丁二醇（BDO）和多羟基丙酸（PHP）的遗传工程微生物。 特别地，本发明涉及适用于通过发酵联合生产PDO，BDO和PHP的遗传工程微生物，其特征在于：与相应的野生型起始微生物相比，遗传上的D-乳酸脱氢酶基因 遗传工程微生物包含编码辅酶A依赖性醛脱氢酶的异源多核苷酸和编码聚羟基链烷酸酯合酶的异源多核苷酸。 还教导了构建这种微生物的方法，以及通过遗传工程化细菌的发酵联合生产PDO，BDO和PHP的方法。