公开(公告)号：US08507250B2

公开(公告)日：2013-08-13

申请号：US13527933

申请日：2012-06-20

申请人： Dehua Liu ,  Hongjuan Liu ,  Xianjin Ou ,  Yan Sun ,  Yunzhen Xu ,  Yueyong Lei ,  Weibin Liu

发明人： Dehua Liu ,  Hongjuan Liu ,  Xianjin Ou ,  Yan Sun ,  Yunzhen Xu ,  Yueyong Lei ,  Weibin Liu

IPC分类号： C12P7/42 ,  C12P7/18 ,  C12N9/00 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12P7/16 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/625 ,  Y02E50/10

摘要： The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.

摘要翻译： 本发明涉及通过发酵联合生产1,3-丙二醇（PDO），2,3-丁二醇（BDO）和多羟基丙酸（PHP）的遗传工程微生物。 特别地，本发明涉及适用于通过发酵联合生产PDO，BDO和PHP的遗传工程微生物，其特征在于：与相应的野生型起始微生物相比，遗传上的D-乳酸脱氢酶基因 遗传工程微生物包含编码辅酶A依赖性醛脱氢酶的异源多核苷酸和编码聚羟基链烷酸酯合酶的异源多核苷酸。 还教导了构建这种微生物的方法，以及通过遗传工程化细菌的发酵联合生产PDO，BDO和PHP的方法。

公开(公告)号：US08227217B2

公开(公告)日：2012-07-24

申请号：US12620142

申请日：2009-11-17

申请人： Dehua Liu ,  Hongjuan Liu ,  Xianjin Ou ,  Yan Sun ,  Yunzhen Xu ,  Yueyong Lei ,  Weibin Liu

发明人： Dehua Liu ,  Hongjuan Liu ,  Xianjin Ou ,  Yan Sun ,  Yunzhen Xu ,  Yueyong Lei ,  Weibin Liu

IPC分类号： C12P7/42 ,  C12P7/18 ,  C12N9/00 ,  C12N15/00

CPC分类号： C12P7/16 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/625 ,  Y02E50/10

摘要： The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.

摘要翻译： 本发明涉及通过发酵联合生产1,3-丙二醇（PDO），2,3-丁二醇（BDO）和多羟基丙酸（PHP）的遗传工程微生物。 特别地，本发明涉及适用于通过发酵联合生产PDO，BDO和PHP的遗传工程微生物，其特征在于：与相应的野生型起始微生物相比，遗传上的D-乳酸脱氢酶基因 遗传工程微生物包含编码辅酶A依赖性醛脱氢酶的异源多核苷酸和编码聚羟基链烷酸酯合酶的异源多核苷酸。 还教导了构建这种微生物的方法，以及通过遗传工程化细菌的发酵联合生产PDO，BDO和PHP的方法。

公开(公告)号：US20110117617A1

公开(公告)日：2011-05-19

申请号：US12620142

申请日：2009-11-17

申请人： Dehua LIU ,  Hongjuan LIU ,  Xianjin OU ,  Yan Sun ,  Yunzhen Xu ,  Yueyong LEI ,  Weibin LIU

发明人： Dehua LIU ,  Hongjuan LIU ,  Xianjin OU ,  Yan Sun ,  Yunzhen Xu ,  Yueyong LEI ,  Weibin LIU

IPC分类号： C12P7/42 ,  C12N1/21 ,  C12P7/18

CPC分类号： C12P7/16 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/625 ,  Y02E50/10

摘要： The present invention relates to methods and genetically engineered micro-organisms for the combined production of PDO, BDO, and PHP by fermentation. The micro-organism is characterized in that its D-lactate dehydrogenase gene has been deleted or functionally inactivated, and it comprises heterogenous polynucleotides encoding the Coenzyme A-dependent Aldehyde dehydrogenase and the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms are also disclosed

摘要翻译： 本发明涉及通过发酵联合生产PDO，BDO和PHP的方法和遗传工程微生物。 微生物的特征在于其D-乳酸脱氢酶基因已被缺失或功能失活，并且其包含编码辅酶A依赖性醛脱氢酶和聚羟基链烷酸酯合酶的异源多核苷酸。 还公开了构建这种微生物的方法

公开(公告)号：US20100028965A1

公开(公告)日：2010-02-04

申请号：US11916180

申请日：2006-06-01

申请人： Dehua Liu ,  hongjuan Liu ,  Yan Sun ,  Rihui Lin ,  Jian Hao

发明人： Dehua Liu ,  hongjuan Liu ,  Yan Sun ,  Rihui Lin ,  Jian Hao

IPC分类号： C12P7/18

CPC分类号： C12P7/18 ,  C12N1/32

摘要： The invention discloses a method for producing 1,3-propanediol, comprising the steps of: using crude glycerol, a by-product during the biodiesel production, without further treatment, as the substrate for production of 1,3-propanediol; inoculating a 1,3-propanediol-producing strain in a seed medium containing crude glycerol, a by-product from biodiesel production; adding the seed culture into a fermentation medium containing crude glycerol, a by-product from biodiesel production, and fermenting; maintaining pH in a range of 6.8 to 8.0; and in the end of the fermentation, isolating and purifying 1,3-propanediol.

摘要翻译： 本发明公开了一种1,3-丙二醇的制造方法，其特征在于，在生物柴油的生产中使用副产物粗甘油，无需进一步处理，作为1,3-丙二醇的生产用基材。 在含有来自生物柴油生产的副产物的粗甘油的种子培养基中接种1,3-丙二醇生产菌株; 将种子培养物加入到含有来自生物柴油生产的副产物和发酵的粗甘油的发酵培养基中; 保持pH在6.8至8.0的范围内; 并在发酵结束时，分离和纯化1,3-丙二醇。

公开(公告)号：US08486673B2

公开(公告)日：2013-07-16

申请号：US11916180

申请日：2006-06-01

申请人： Dehua Liu ,  Hongjuan Liu ,  Yan Sun ,  Rihui Lin ,  Jian Hao

发明人： Dehua Liu ,  Hongjuan Liu ,  Yan Sun ,  Rihui Lin ,  Jian Hao

IPC分类号： C12P7/18

CPC分类号： C12P7/18 ,  C12N1/32

摘要： The invention discloses a method for producing 1,3-propanediol, comprising the steps of: using crude glycerol, a by-product during the biodiesel production, without further treatment, as the substrate for production of 1,3-propanediol; inoculating a 1,3-propanediol-producing strain in a seed medium containing crude glycerol, a by-product from biodiesel production; adding the seed culture into a fermentation medium containing crude glycerol, a by-product from biodiesel production, and fermenting; maintaining pH in a range of 6.8 to 8.0; and in the end of the fermentation, isolating and purifying 1,3-propanediol.

摘要翻译： 本发明公开了一种1,3-丙二醇的制造方法，其特征在于，在生物柴油的生产中使用副产物粗甘油，无需进一步处理，作为1,3-丙二醇的生产用基材。 在含有来自生物柴油生产的副产物的粗甘油的种子培养基中接种1,3-丙二醇生产菌株; 将种子培养物加入到含有来自生物柴油生产的副产物和发酵的粗甘油的发酵培养基中; 保持pH在6.8至8.0的范围内; 并在发酵结束时，分离和纯化1,3-丙二醇。

公开(公告)号：US20160014118A1

公开(公告)日：2016-01-14

申请号：US14790269

申请日：2015-07-02

申请人： Bin Da ,  Wei Wang ,  Linju Yang ,  Ke Liao ,  Yan Sun

发明人： Bin Da ,  Wei Wang ,  Linju Yang ,  Ke Liao ,  Yan Sun

IPC分类号： H04L29/06

CPC分类号： H04L63/0853 ,  H04L63/10 ,  H04L63/107 ,  H04L63/123

摘要： Disclosed is an access control method of an area restricted network system including a central control device and at least one area restricted network. Each area restricted network includes at least a master node. The method comprises a detection step of detecting that an access device has entered into an area restricted network in the area restricted network system; a receipt step of receiving, by a master node in the area restricted network, an authentication information request sent by the access device; a sending step of, in response to the authentication information request, generating and sending the authentication information for the access device by the master node in the area restricted network; and a request step of, by the access device, receiving the authentication information, and then utilizing the authentication information so as to send an authentication request to the central control device.

摘要翻译： 公开了一种包括中央控制装置和至少一个区域限制网络的区域限制网络系统的访问控制方法。 每个区域受限网络至少包括主节点。 该方法包括：检测步骤，检测接入设备已经进入区域限制网络系统中的区域限制网络; 接收步骤，由所述区域限制网络中的主节点接收由所述接入设备发送的认证信息请求; 发送步骤，响应于所述认证信息请求，生成并发送所述区域受限网络中的所述主节点对所述接入设备的认证信息; 以及由访问装置接收认证信息，然后利用认证信息以便向中央控制装置发送认证请求的请求步骤。

公开(公告)号：US09226336B2

公开(公告)日：2015-12-29

申请号：US14168118

申请日：2014-01-30

申请人： Yan Sun ,  Wei Wang ,  Bin Da ,  Linju Yang ,  Haihua Yu

发明人： Yan Sun ,  Wei Wang ,  Bin Da ,  Linju Yang ,  Haihua Yu

IPC分类号： H04W84/02 ,  H04W12/04 ,  H04L9/08 ,  H04W84/12 ,  H04W4/00 ,  H04W4/02 ,  H04L29/06

CPC分类号： H04W84/12 ,  H04L9/08 ,  H04L63/0846 ,  H04W4/021 ,  H04W4/80 ,  H04W12/04

摘要： Disclosed are a wireless network area limiting method and a wireless network area limiting system based on NFC. The wireless network area limiting method includes a step of adopting a plurality of first NFC hot spot sensors to seamlessly define a limited area having any shape; a step of periodically generating and transmitting an area secret key by a first central control part respectively connected to the plurality of first NFC hot spot sensors; and a step of conducting handshake with the first central control part by a WLAN user equipment, which has a NFC function and is located in the limited area, based on the area secret key received by the WLAN user equipment, so that the first central control part establishes area-limited network communications in the limited area.

摘要翻译： 公开了一种基于NFC的无线网络区域限制方法和无线网络区域限制系统。 无线网络区域限制方法包括采用多个第一NFC热点传感器来无缝地限定具有任何形状的有限区域的步骤; 通过分别连接到多个第一NFC热点传感器的第一中央控制部周期地产生和发送区域密钥的步骤; 以及基于由WLAN用户设备接收的区域密钥，通过具有NFC功能并位于有限区域的WLAN用户设备与第一中央控制部分进行握手的步骤，使得第一中央控制 部分在有限的区域建立了区域有限的网络通信。

公开(公告)号：US09055436B2

公开(公告)日：2015-06-09

申请号：US13874671

申请日：2013-05-01

申请人： Yan Sun ,  Wei Wang ,  Haihua Yu ,  Bin Da

发明人： Yan Sun ,  Wei Wang ,  Haihua Yu ,  Bin Da

IPC分类号： H04W12/04 ,  H04B10/114

CPC分类号： H04W12/04 ,  H04B10/1149 ,  H04L9/0869 ,  H04L9/12 ,  H04L63/18 ,  H04L2209/08 ,  H04L2209/34 ,  H04L2209/80 ,  H04L2463/061 ,  H04W84/12

摘要： A transmitting device, a receiving device, a wireless communication system and a method for controlling the wireless communication system are used for creating a secure wireless communication network of a limited area. The transmitting device comprises: one or more transmitters configured to transmit wireless signals, and create the limited area by a coverage scope of the wireless signals; a cryptography key generating unit configured to generate a cryptography key for the wireless signals; a cryptography key encoding unit configured to encode the cryptography key to a cryptography key frame; and a cryptography key scheduling unit configured to schedule the cryptography key frame so as to control the transmitter to transmit the wireless signals including the cryptography key frame.

摘要翻译： 使用发送设备，接收设备，无线通信系统和用于控制无线通信系统的方法来创建有限区域的安全无线通信网络。 发射设备包括：一个或多个发射机，被配置为发射无线信号，并通过无线信号的覆盖范围创建有限区域; 密码产生单元，被配置为生成所述无线信号的密码密钥; 密码密钥编码单元，被配置为将加密密钥编码为密码关键帧; 以及密码密钥调度单元，被配置为调度密码关键帧，以便控制发送器发送包括密码关键帧的无线信号。

公开(公告)号：US20110055104A1

公开(公告)日：2011-03-03

申请号：US12909062

申请日：2010-10-21

申请人： Yan Sun ,  Steven M. Kay ,  Yafei Yang ,  Qing Yang

发明人： Yan Sun ,  Steven M. Kay ,  Yafei Yang ,  Qing Yang

IPC分类号： G06Q30/00

CPC分类号： G06Q30/02 ,  G06Q10/10 ,  G06Q30/0282

摘要： A method is disclosed for detecting unfair ratings in rating data over a period of time in connection with an on-line rating system. The method includes the steps of: detecting changes in an arrival rate of ratings in the rating data over the period of time and providing arrival rate change data; detecting changes in a model of the rating data over the period of time such that changes in the model are represented as model errors and providing model error data; detecting changes in a histogram of the rating data over the period of time and providing histogram detection data; detecting changes in a mean of the rating data over the period of time and providing mean change detection data; and processing the arrival rate change data, the model error data, the histogram detection data, and the mean change detection data to identify unfair ratings in the rating data.

摘要翻译： 公开了一种用于在一段时间内检测与在线评级系统相关的评级数据中的不公平等级的方法。 该方法包括以下步骤：检测一段时间内评级数据中的评级到达率的变化并提供到达率变化数据; 在时间段内检测评级数据模型的变化，使得模型中的变化被表示为模型误差并提供模型误差数据; 检测一段时间内评级数据的直方图的变化并提供直方图检测数据; 检测一段时间内评级数据的平均值的变化并提供均值变化检测数据; 以及处理到达率变化数据，模型误差数据，直方图检测数据和平均变化检测数据，以识别评级数据中的不公平评级。

公开(公告)号：US06498677B1

公开(公告)日：2002-12-24

申请号：US09729082

申请日：2000-12-05

申请人： Yan Sun ,  Jun Ye

发明人： Yan Sun ,  Jun Ye

IPC分类号： H04B1012

CPC分类号： H04B10/296

摘要： Optical amplifiers are provided for use in fiber-optic communications networks. Gain may be provided using one or more rare-earth-doped fiber coils such as erbium-doped fiber coils. The coils may be pumped by laser diodes or other suitable pumps. The optical output power of the pumps in a given amplifier may be controlled by a control unit. Taps may be used to monitor the power of optical signals being amplified by the amplifier. The tapped optical signals may be spectrally-filtered. The control unit may calculate the appropriate pump power for the pumps to supply to the fiber coils based on the measured spectrally-filtered optical signals. The gain of the amplifier may be maintained at a desired level using feedback control techniques. The gain spectrum of the amplifier need not be flat. A combination of feed-forward and feedback techniques may be used to calculate the pump power to be supplied by the pumps.

摘要翻译： 提供光放大器用于光纤通信网络。 可以使用一个或多个稀土掺杂的光纤线圈（例如掺铒光纤线圈）来提供增益。 线圈可以由激光二极管或其它合适的泵泵送。 给定放大器中的泵的光输出功率可以由控制单元控制。 抽头可用于监视被放大器放大的光信号的功率。 抽头的光信号可以进行频谱滤波。 控制单元可以基于经测量的经过光谱滤波的光信号，计算供给纤维线圈的泵的适当泵功率。 可以使用反馈控制技术将放大器的增益保持在期望的水平。 放大器的增益谱不必平坦。 可以使用前馈和反馈技术的组合来计算由泵供应的泵功率。