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公开(公告)号:US08906644B2
公开(公告)日:2014-12-09
申请号:US13392226
申请日:2010-07-28
申请人: Hitoshi Matsui , Yasuyuki Sakai , Teruo Fujii , Shoji Takeuchi , Yukiko Tsuda
发明人: Hitoshi Matsui , Yasuyuki Sakai , Teruo Fujii , Shoji Takeuchi , Yukiko Tsuda
CPC分类号: C12M23/20 , C12M23/24 , C12M25/14 , C12M41/46 , C12N5/0671 , C12N2503/00 , C12N2533/30 , C12N2533/54 , G01N33/5044
摘要: A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.
摘要翻译: 一种培养肝细胞的方法,其中将包埋在细胞外基质中的肝细胞置于透气膜上,并且在从透气膜侧供给氧的同时培养肝细胞。 由此,可以诱导肝细胞中的极性,并且可以在短时间内形成胆小管。 此外,所形成的极性可以保持更长的时间。
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公开(公告)号:US20120183989A1
公开(公告)日:2012-07-19
申请号:US13392226
申请日:2010-07-28
申请人: Hitoshi Matsui , Yasuyuki Sakai , Teruo Fujii , Shoji Takeuchi , Yukiko Tsuda
发明人: Hitoshi Matsui , Yasuyuki Sakai , Teruo Fujii , Shoji Takeuchi , Yukiko Tsuda
CPC分类号: C12M23/20 , C12M23/24 , C12M25/14 , C12M41/46 , C12N5/0671 , C12N2503/00 , C12N2533/30 , C12N2533/54 , G01N33/5044
摘要: A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.
摘要翻译: 一种培养肝细胞的方法,其中将包埋在细胞外基质中的肝细胞置于透气膜上,并且在从透气膜侧供给氧的同时培养肝细胞。 由此,可以诱导肝细胞中的极性,并且可以在短时间内形成胆小管。 此外,所形成的极性可以保持更长的时间。
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