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    Cell culture substrate and its production method
    3.
    发明授权
    Cell culture substrate and its production method 有权
    细胞培养基质及其制备方法

    公开(公告)号：US08383409B2

    公开(公告)日：2013-02-26

    申请号：US13020193

    申请日：2011-02-03

    申请人： Shin Ohya ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

    发明人： Shin Ohya ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

    IPC分类号： C12N5/02

    CPC分类号： C12N5/0068 ,  C08G2261/3424 ,  C08L65/04 ,  C12N2533/30

    摘要： The invention is directed to methods for the propagation or cultivation of cells including preparing a cell culture substrate, wherein the cell culture substrate includes a substrate and a layer formed by surface modification. The layer includes a polymer containing an amino group. The polymer is produced by reacting a polymer represented by formula (II): with a polymer having at least one amino group, —NH2, capable of forming a Schiff base in a monomer of formula (II), thereby forming a polymer layer constituting the layer formed by surface modification. “n” in Formula (II) is 0 or a positive integer, and m is a positive integer. n and m represent the degree of polymerization. Formula (II) is formed by chemical vapor deposition of formyl[2.2]paracyclophane. The methods further include providing cells in a medium; inoculating the cells onto the cell culture substrate; and culturing the cells, wherein the cells adhere to the cell culture substrate.

    摘要翻译： 本发明涉及细胞繁殖或培养方法，包括制备细胞培养基质，其中细胞培养基质包括基质和通过表面改性形成的层。 该层包括含有氨基的聚合物。 该聚合物通过使由式（II）表示的聚合物与具有至少一个氨基的聚合物-NH 2反应生成，该聚合物能够在式（II）的单体中形成席夫碱，从而形成构成 通过表面改性形成的层。 式（II）中的n为0或正整数，m为正整数。 n和m表示聚合度。 式（II）由甲酰基[2.2]对环磷酰胺的化学气相沉积形成。 所述方法还包括在培养基中提供细胞; 将细胞接种到细胞培养基底上; 并培养细胞，其中细胞粘附于细胞培养基质。

    Cell culture substrate and its production method
    4.
    发明申请
    Cell culture substrate and its production method 审中-公开
    细胞培养基质及其制备方法

    公开(公告)号：US20090042295A1

    公开(公告)日：2009-02-12

    申请号：US11889067

    申请日：2007-08-08

    申请人： Shin Ohya ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

    发明人： Shin Ohya ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0068 ,  C08G2261/3424 ,  C08L65/04 ,  C12N2533/30

    摘要： A cell culture substrate which is durable and which can be readily produced in commercial scale at a low cost, and its production method are provided. The cell culture substrate comprises a substrate and a layer formed by surface modification, which comprises a polymer containing amino group produced by reacting a polymer represented by the following formula (II): (wherein n is 0 or a positive integer, and m is a positive integer, the n and m representing degree of polymerization) formed by chemical vapor deposition of formyl[2.2]paracyclophane represented by the following formula (I): (wherein k is 0 or 1) with a polymer having at least one amino group (—NH2) capable of forming Schiff base in its monomer. The production method of the cell culture substrate comprises the step of synthesizing such polymer on the substrate.

    摘要翻译： 提供耐久性且能够以低成本容易地以商业规模生产的细胞培养基材及其制造方法。 细胞培养基材包括基材和通过表面改性形成的层，其包含通过使由下式（II）表示的聚合物反应而生成的含氨基的聚合物：（其中n为0或正整数，m为 正整数，代表聚合度的n和m）通过化学气相沉积由下式（I）表示的甲酰基[2.2]对环烷烃形成：（其中k为0或1）与具有至少一个氨基的聚合物 -NH2）能够在其单体中形成席夫碱。 细胞培养基质的制造方法包括在基材上合成这样的聚合物的工序。

    Electrical signal measuring device for cells in culture and electrical signal measuring method that uses same device
    5.
    发明申请
    Electrical signal measuring device for cells in culture and electrical signal measuring method that uses same device 审中-公开
    用于培养细胞的电信号测量装置和使用相同装置的电信号测量方法

    公开(公告)号：US20070212773A1

    公开(公告)日：2007-09-13

    申请号：US11707588

    申请日：2007-02-15

    申请人： Teruo Fujii ,  Yasuyuki Sakai ,  Serge Ostrovidov

    发明人： Teruo Fujii ,  Yasuyuki Sakai ,  Serge Ostrovidov

    IPC分类号： C12M1/34

    CPC分类号： C12M41/36 ,  C12M29/04 ,  C12M29/10 ,  C12M41/46 ,  G01N33/4833

    摘要： An electrical signal measuring device that can measure electrical characteristics of cells in culture in real time and with high accuracy while maintaining them in a favorable state and an electrical signal measuring method that uses the device are disclosed. The electrical signal measuring device 1 for cells in culture includes a support body 2, a compartment in the support body, a semipermeable membrane 3, and electrodes, in which the compartment is divided by the semipermeable membrane 3 into an upper compartment 4 and a lower compartment 5; an upper electrode 61 is provided in the upper compartment 4, and a lower electrode 62 that faces the upper electrode 61 and whose facing surface 621 is in contact with the semipermeable membrane 3 is provided in the lower compartment 5; and an upper perfusion channel and a lower perfusion channel are provided in the support body 2 to separately perfuse fluid in the upper compartment 4 and the lower compartment 5 is disclosed.

    摘要翻译： 公开了一种电信号测量装置，其能够实时且准确地测量培养中的细胞的电特性，同时保持它们处于良好状态，并且使用该装置的电信号测量方法。 用于培养细胞的电信号测量装置1包括支撑体2，支撑体中的隔室，半透膜3和电极，其中隔室被半透膜3分成上隔室4和下隔室 隔间5; 在上部隔间4设置有上部电极61，并且在下部隔室5中设置面向上部电极61并且其面对面621与半透膜3接触的下部电极62; 并且在支撑体2中设置有上部灌注通道和下部灌注通道，以分开地灌注上部隔室4中的流体，并且公开了下部隔室5。

    CELL CULTURE SUBSTRATE AND ITS PRODUCTION METHOD
    6.
    发明申请
    CELL CULTURE SUBSTRATE AND ITS PRODUCTION METHOD 有权
    细胞培养基质及其生产方法

    公开(公告)号：US20110136234A1

    公开(公告)日：2011-06-09

    申请号：US13020193

    申请日：2011-02-03

    申请人： Shin OHYA ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

    发明人： Shin OHYA ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

    IPC分类号： C12N5/07 ,  C12N5/0735

    CPC分类号： C12N5/0068 ,  C08G2261/3424 ,  C08L65/04 ,  C12N2533/30

    摘要： A cell culture substrate which is durable and which can be readily produced in commercial scale at a low cost, and its production method are provided. The cell culture substrate comprises a substrate and a layer formed by surface modification, which comprises a polymer containing amino group produced by reacting a polymer represented by the following formula (II): (wherein n is 0 or a positive integer, and m is a positive integer, the n and m representing degree of polymerization) formed by chemical vapor deposition of formyl[2.2]paracyclophane represented by the following formula (I): (wherein k is 0 or 1) with a polymer having at least one amino group (—NH2) capable of forming Schiff base in its monomer. The production method of the cell culture substrate comprises the step of synthesizing such polymer on the substrate.

    摘要翻译： 提供耐久性且能够以低成本容易地以商业规模生产的细胞培养基材及其制造方法。 细胞培养基材包括基材和通过表面改性形成的层，其包含通过使由下式（II）表示的聚合物反应而生成的含氨基的聚合物：（其中n为0或正整数，m为 正整数，代表聚合度的n和m）通过化学气相沉积由下式（I）表示的甲酰基[2.2]对环烷烃形成：（其中k为0或1）与具有至少一个氨基的聚合物 -NH2）能够在其单体中形成席夫碱。 细胞培养基质的制造方法包括在基材上合成这样的聚合物的工序。

    Cell Culture Chamber
    7.
    发明申请
    Cell Culture Chamber 审中-公开
    细胞培养室

    公开(公告)号：US20080145925A1

    公开(公告)日：2008-06-19

    申请号：US11885889

    申请日：2005-12-08

    申请人： Yasuyuki Sakai ,  Teruo Fujii ,  Serge Ostrovidov ,  Hiroaki Inui ,  Jinji Mizuno

    发明人： Yasuyuki Sakai ,  Teruo Fujii ,  Serge Ostrovidov ,  Hiroaki Inui ,  Jinji Mizuno

    IPC分类号： C12M1/12

    CPC分类号： C12M35/08 ,  C12M23/34 ,  C12M29/10

    摘要： A cell culture chamber includes: a top compartment and a bottom compartment that are separated by a semi-permeable membrane inside a supporting body; a culture solution supply flow path that supplies culture solution to an interior of the bottom compartment; a culture solution discharge flow path that discharges culture solution from the interior of the bottom compartment; a circulation flow path that is used to circulate fluid within the interior of the top compartment; and a cell culturing portion is formed inside the top compartment by a sieve structure that is formed by aperture portions through which a fluid is able to pass but through which cultured cells cannot pass, and by wall portions.

    摘要翻译： 细胞培养室包括：顶部隔室和底部隔室，其由支撑体内的半透膜隔开; 将培养液供给到所述底部室的内部的培养液供给流路; 从底部室的内部排出培养液的培养液排出流路; 用于使流体在顶部隔间的内部循环的循环流动路径; 并且细胞培养部分通过筛孔结构形成在顶部隔室内，该筛结构由流体能够通过的通孔，通过培养的细胞不能通过的孔部分和壁部形成。

    METHOD AND DEVICE FOR SYNTHESIZING PROTEIN FROM DNA MOLECULE CAPTURED IN MICROCHAMBER
    8.
    发明申请
    METHOD AND DEVICE FOR SYNTHESIZING PROTEIN FROM DNA MOLECULE CAPTURED IN MICROCHAMBER 审中-公开
    用于从蛋白质捕获的DNA分子合成蛋白质的方法和装置

    公开(公告)号：US20130165348A1

    公开(公告)日：2013-06-27

    申请号：US13513896

    申请日：2010-12-08

    申请人： Teruo Fujii ,  Soo-Hyeon Kim ,  Shoji Takeuchi ,  Dominique Fourmy ,  Satoko Yoshizawa

    发明人： Teruo Fujii ,  Soo-Hyeon Kim ,  Shoji Takeuchi ,  Dominique Fourmy ,  Satoko Yoshizawa

    IPC分类号： C40B50/08 ,  C40B60/14

    CPC分类号： C12P21/02

    摘要： Provided are a method and a device for synthesizing proteins from DNA molecules captured in microchambers, whereby the distance between microchambers can be shortened, thereby allowing the density of arrays to be increased. Microchambers (32) are arranged at a high density. A DNA solution (34) which has been diluted so as to capture one DNA molecule on average is enclosed in the microchambers (32). Then, mRNA is synthesized using one DNA molecule on average as a template. Based on this mRNA, a protein (37) is extracellularly synthesized.

    摘要翻译： 本发明提供了一种用于从微室中捕获的DNA分子合成蛋白质的方法和装置，由此能够缩短微室之间的距离，从而增加阵列的密度。 微型仓（32）以高密度排列。 已经稀释以便平均捕获一个DNA分子的DNA溶液（34）被包封在微室（32）中。 然后，使用一个DNA分子平均作为模板合成mRNA。 基于该mRNA，蛋白质（37）在细胞外合成。

    Transmission illumination device in a picture scanning device
    9.
    发明授权
    Transmission illumination device in a picture scanning device 失效
    传输照明装置在图像扫描装置中

    公开(公告)号：US4558214A

    公开(公告)日：1985-12-10

    申请号：US529605

    申请日：1983-09-06

    申请人： Teruo Fujii ,  Mikizo Katsuyama

    发明人： Teruo Fujii ,  Mikizo Katsuyama

    IPC分类号： G02B27/00 ,  G03F1/00 ,  G03F1/90 ,  H04N1/028 ,  H04N1/029 ,  H04N1/04 ,  H04N1/407 ,  H01J3/14

    CPC分类号： H04N1/0282 ,  H04N1/02815 ,  H04N1/029

    摘要： Disclosed is an improved transmission illumination device for use in a picture scanning device such as an electronic picture scanner which can illuminate an annular region around an illuminated spot. This annular illuminated spot is produced by using an annular convex lens as part of an optical system. Since the area surrounding the illuminated spot is also illuminated, finding a desired spot on an original picture is greatly facilitated. Having an illuminated annular region does not create any problem which may arise from flare light if an iris diaphragm is provided in a light receiving head for the exclusion of the light emitted from the illuminated annular area.

    摘要翻译： 公开了一种用于诸如电子图像扫描器的图像扫描装置的改进的透射照明装置，其能够照亮照明点周围的环形区域。 该环形照明光点通过使用环形凸透镜作为光学系统的一部分而产生。 由于照亮点周围的区域也被照亮，所以在原始图像上找到所需的位置被大大地简化。 如果在光接收头中设置虹膜隔膜以排除从照明的环形区域发射的光，则具有照明的环形区域不会产生可能由火炬光产生的任何问题。