公开(公告)号：US20080227165A1

公开(公告)日：2008-09-18

申请号：US12082917

申请日：2008-04-15

申请人： Naomi Mizusawa ,  Masashi Sakai ,  Satoshi Kudo ,  Yukio Shirasawa

发明人： Naomi Mizusawa ,  Masashi Sakai ,  Satoshi Kudo ,  Yukio Shirasawa

IPC分类号： C12P7/64 ,  C07C57/12

CPC分类号： C12P7/6427 ,  A23L33/12 ,  A23L33/135 ,  A23V2002/00 ,  A23Y2220/69 ,  A23Y2300/59 ,  C11C1/00 ,  C11C3/14 ,  C12R1/01 ,  C12R1/225 ,  A23V2250/1866 ,  A23V2250/61 ,  A23V2250/5488 ,  A23V2250/5424

摘要： A conjugated fatty acid such as the cis-9, trans-11 isomer of conjugated linoleic acid having high bioactivities is selectively and efficiently produced by conjugating an unsaturated fatty acid having at least two double bonds with the use of viral cells, dead cells or a cell extract of at least one bacterium selected from the group consisting of Lactobacillus oris, Lactobacillus pontis, Lactobacillus panis, Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium infantis and Bifidobacterium pseudocatenulatum, or an enzyme derived from the bacterium.

摘要翻译： 共轭脂肪酸如具有高生物活性的共轭亚油酸的顺式-9反式-11异构体通过与具有至少两个双键的不饱和脂肪酸结合使用病毒细胞，死细胞或 或选自以下的至少一种细菌的细胞提取物：乳酸乳杆菌（Lactobacillus oris），粘乳杆菌（Lactobacillus pontis），芸苔属双歧杆菌（Bifidobacterium breve），双歧双歧杆菌（Bifidobacteriumbifidum），婴儿双歧杆菌（Bifidobacterium infantis）和假双孢杆菌（Bifidobacterium pseudocatenulatum）或源自细菌的酶。

公开(公告)号：US06319692B1

公开(公告)日：2001-11-20

申请号：US09202893

申请日：1998-12-22

申请人： Mariko Kadota ,  Mayumi Kiwaki ,  Saeko Sawaki ,  Yukio Shirasawa ,  Harue Sone ,  Tomoyuki Sako

发明人： Mariko Kadota ,  Mayumi Kiwaki ,  Saeko Sawaki ,  Yukio Shirasawa ,  Harue Sone ,  Tomoyuki Sako

IPC分类号： C12P1934

CPC分类号： C12N15/907 ,  C12N15/746 ,  C12N15/90

摘要： Methods for transferring a foreign gene into a host chromosome by integrating the foreign gene into a vector by utilizing a lysogenic phage, for the purpose of deleting unnecessary genes derived from the vector, except for the foreign gene. One method comprises (a) preparing a vector with a lysogenic phage integration site (such as an attP site) arranged between a first partial sequence of the objective foreign gene to be transferred, but lacking one terminal region thereof and a second partial sequence of the gene, but lacking the other terminal region, the second partial sequence having an overlapping region with a portion of the first partial sequence; (b) integrating the vector obtained in step (a) into the host chromosome; and (c) screening from among the recombinants obtained in step (b) a recombinant from which unnecessary genes originating in the vector have been deleted owing to the homologous recombination mechanism functioning in the overlapping region between the first and second partial sequences.

摘要翻译： 通过利用溶源性噬菌体将外源基因整合到载体中，为了除去外来基因以外的来自载体的不必要的基因，将外源基因转移到宿主染色体中的方法。 一种方法包括（a）制备载体，该载体具有排列在要转移的目标外源基因的第一部分序列之间，但缺少其一个末端区域和第二部分序列的溶源性噬菌体整合位点（例如attP位点） 基因，但缺少另一个末端区域，第二部分序列与第一部分序列的一部分具有重叠区域; （b）将步骤（a）中获得的载体整合到宿主染色体中; 和（c）从步骤（b）中获得的重组体中筛选来自该载体的不必要的基因由于第一和第二部分序列之间的重叠区域中的同源重组机制起作用而被缺失的重组体。

公开(公告)号：US20050208195A1

公开(公告)日：2005-09-22

申请号：US10511230

申请日：2003-04-11

申请人： Naomi Mizusawa ,  Masashi Sakai ,  Satoshi Kudo ,  Yukio Shirasawa

发明人： Naomi Mizusawa ,  Masashi Sakai ,  Satoshi Kudo ,  Yukio Shirasawa

IPC分类号： A23C9/123 ,  A23L1/30 ,  C11C1/00 ,  C11C3/14 ,  C12P7/40 ,  C12P7/64 ,  A23D7/00

CPC分类号： C12P7/6427 ,  A23L33/12 ,  A23L33/135 ,  A23V2002/00 ,  A23Y2220/69 ,  A23Y2300/59 ,  C11C1/00 ,  C11C3/14 ,  C12R1/01 ,  C12R1/225 ,  A23V2250/1866 ,  A23V2250/61 ,  A23V2250/5488 ,  A23V2250/5424

摘要： A conjugated fatty acid such as the cis-9, trans-11 isomer of conjugated linoleic acid having high bioactivities is selectively and efficiently produced by conjugating an unsaturated fatty acid having at least two double bonds with the use of viral cells, dead cells or a cell extract of at least one bacterium selected from the group consisting of Lactobacillus oris, Lactobacillus pontis, Lactobacillus panis, Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium infantis and Bifidobacterium pseudocatenulatum, or an enzyme derived from the bacterium.

摘要翻译： 共轭脂肪酸如具有高生物活性的共轭亚油酸的顺式-9反式-11异构体通过与具有至少两个双键的不饱和脂肪酸结合使用病毒细胞，死细胞或 或选自以下的至少一种细菌的细胞提取物：乳酸乳杆菌（Lactobacillus oris），粘乳杆菌（Lactobacillus pontis），芸苔属双歧杆菌（Bifidobacterium breve），双歧双歧杆菌（Bifidobacteriumbifidum），婴儿双歧杆菌（Bifidobacterium infantis）和假双孢杆菌（Bifidobacterium pseudocatenulatum）或源自细菌的酶。