公开(公告)号：US08283154B2

公开(公告)日：2012-10-09

申请号：US12561078

申请日：2009-09-16

申请人： Kazuhiro Takagi ,  Naoki Harada ,  Yuuichi Yoshioka

发明人： Kazuhiro Takagi ,  Naoki Harada ,  Yuuichi Yoshioka

IPC分类号： A62D3/02 ,  C12N11/14 ,  C12N11/02 ,  C12N11/04

CPC分类号： C12P39/00 ,  B09C1/002 ,  B09C1/10 ,  C12N11/02 ,  C12N11/12 ,  C12R1/01 ,  C12R1/06 ,  Y10S435/822 ,  Y10S435/83

摘要： The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed accumulation of degrading bacteria, which contains a porous material provided as a support on which a degrading bacterium A capable of degrading at least one organic contaminant and a degrading bacterium B capable of degrading another organic contaminant are accumulated, is produced. The degrading bacterium A may be a PCNB-degrading bacterium, particularly a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of Nocardioides sp. PD653 and the degrading bacterium B may be a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of β-Proteobacteria CDB21.

摘要翻译： 本发明提供了一种稳定的复合微生物系统，即使在这些污染物的污染环境下也能同时分解多种有机污染物，并允许持久性有机污染物如PCNB和西马嗪的更有效分解。 产生含有多余材料的支持物，其含有多孔材料作为载体，所述多孔材料在其上堆积能够降解至少一种有机污染物的降解菌A和能够降解另一种有机污染物的降解菌B 。 降解菌A可以是PCNB降解细菌，特别是含有具有诺卡氏菌属的真菌学特征的部分或全部的降解菌的降解菌。 PD653和降解菌B可以是含有降解菌的降解细菌，其具有部分或全部具有细菌CDB21的真菌学特征。

公开(公告)号：US06498028B1

公开(公告)日：2002-12-24

申请号：US09462877

申请日：2000-03-24

申请人： Kazuhiro Takagi ,  Yuuichi Yoshioka

发明人： Kazuhiro Takagi ,  Yuuichi Yoshioka

IPC分类号： C12N120

CPC分类号： C12N1/20 ,  B09C1/10 ,  C12R1/01

摘要： It is an object of the invention to improve a conventional soil percolation technique to thereby provide a method of enriching and isolating decomposing bacteria decomposing an organochlorine agricultural chemical PCNB which is difficult to decompose, in a short time period, and to provide decomposing bacteria for efficiently processing PCNB. To practice the method, an enrichment soil layer 2 is formed by mixing a soil containing an organochlorine agricultural chemical PCNB with a fragmented porous material having an infinite number of micropores and at the same time a greater adsorptivity for adsorbing PCNB than the soil, and an inorganic salt medium 3 containing a carbon and nitrogen source, the carbon and nitrogen source being formed by only PCNB, is circulated through the enrichment soil layer 2, thereby enriching the aerobic bacteria Burkholderia cepacia in the fragmented porous material.

摘要翻译： 本发明的目的是改进常规的土壤渗滤技术，从而提供一种在短时间内分解分解有机氯农药PCNB的分解细菌的分解方法，并且能够有效地提供分解细菌 处理PCNB。 为了实施该方法，通过将含有机氯农药PCNB的土壤与具有无数个微孔的碎裂多孔材料混合并同时具有比土壤吸附PCNB更大的吸附性，形成富集土层2， 含有碳源和氮源的无机盐介质3，仅由PCNB形成的碳源和氮源通过富集土层2循环，从而富集碎片多孔材料中的好氧细菌伯克霍尔德菌（Burkholderia cepacia）。

公开(公告)号：US06569333B1

公开(公告)日：2003-05-27

申请号：US09744996

申请日：2001-04-20

申请人： Kazuhiro Takagi ,  Yuuichi Yoshioka

发明人： Kazuhiro Takagi ,  Yuuichi Yoshioka

IPC分类号： C02F300

CPC分类号： B09C1/10 ,  C12N11/02

摘要： The object of the present invention is to restore soil and prevent contamination of ground water by decomposing an organic compound with decomposing microorganisms in soil contaminated with an agricultural chemical or other organic compound. The present invention discloses an accumulation method for isolated decomposing microorganisms comprising: accumulating decomposing microorganisms of a species able to decompose a specific organic compound in a porous material that is able to adsorb said organic compound while also having a countless number of pores that facilitate habitation by said decomposing microorganisms; wherein, by inoculating only said decomposing microorganisms into a porous material, forming an accumulation layer composed of that inoculated porous material, and perfusing that accumulation layer with an inorganic salt medium having only said organic compound for its carbon source and nitrogen source, said decomposing microorganisms are accumulated in the porous material in a state of high density, and a decomposing microorganism retentive carrier obtained in this manner is buried directly in contaminated soil.

摘要翻译： 本发明的目的是通过在被农药或其他有机化合物污染的土壤中分解微生物分解有机化合物来恢复土壤并防止污染地下水。本发明公开了一种分离分解微生物的积累方法，包括： 能够分解能够吸附所述有机化合物的多孔材料中的特定有机化合物的物质的微生物分解，同时具有无数个孔，所述孔有利于所述分解微生物的栖息; 其中，通过仅将所述分解微生物接种到多孔材料中，形成由该接种的多孔材料构成的蓄积层，并且用仅具有所述有机化合物的无机盐介质用于其碳源和氮源灌注该积聚层，所述分解微生物 以高密度的状态积聚在多孔材料中，以这种方式获得的分解微生物保持性载体直接埋入污染的土壤中。

公开(公告)号：US06451580B1

公开(公告)日：2002-09-17

申请号：US09378762

申请日：1999-08-23

申请人： Kazuhiro Takagi ,  Yuuichi Yoshioka

发明人： Kazuhiro Takagi ,  Yuuichi Yoshioka

IPC分类号： B09B300

CPC分类号： B09C1/10 ,  C12N11/14

摘要： A porous material having a greater adsorptivity for adsorbing organic contaminants, such as agricultural chemicals, than a target soil, is fragmented. The fragmented porous material is mixed into a soil which the decomposing bacteria inhabit, thereby forming an enrichment soil layer. Through the enrichment soil layer, there is circulated an inorganic salt medium containing carbon and nitrogen sources formed by only an organic contaminant to be decomposed, thereby rapidly enriching the decomposing bacteria in the fragmented porous material. The fragmented porous material is inoculated into new fragmented porous material to form an enrichment layer consisting of the fragmented porous material only. Into the enrichment layer, the inorganic salt medium containing carbon and nitrogen sources formed by only an organic contaminant to be decomposed is circulated and the operation of enriching decomposing bacteria is repeated a plurality of times for the new fragmented porous material as well to enhance the degree of purity and enrichment of the decomposing bacteria, thereby rapidly isolating the decomposing bacteria.

摘要翻译： 与目标土壤相比，吸附有机污染物（如农药）的吸附性更高的多孔材料是碎片化的。 将分散的多孔材料混合到分解细菌所在的土壤中，从而形成富集土层。 通过富集土层循环含有由仅分解有机污染物形成的碳源和氮源的无机盐介质，从而快速富集碎裂多孔材料中的分解菌。 将碎裂的多孔材料接种到新的碎片多孔材料中以形成仅由碎片多孔材料组成的富集层。 向富集层中循环仅由有机污染物形成的含有碳源和氮源的无机盐介质进行分解，对于新的碎裂多孔质材料，多次重复分解细菌的富集操作，提高其分解度 的分解细菌的纯度和富集，从而快速分离分解细菌。

公开(公告)号：US20100006499A1

公开(公告)日：2010-01-14

申请号：US12561078

申请日：2009-09-16

申请人： Kazuhiro Takagi ,  Naoki Harada ,  Yuuichi Yoshioka

发明人： Kazuhiro Takagi ,  Naoki Harada ,  Yuuichi Yoshioka

IPC分类号： C02F3/34

CPC分类号： C12P39/00 ,  B09C1/002 ,  B09C1/10 ,  C12N11/02 ,  C12N11/12 ,  C12R1/01 ,  C12R1/06 ,  Y10S435/822 ,  Y10S435/83

摘要： The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed accumulation of degrading bacteria, which contains a porous material provided as a support on which a degrading bacterium A capable of degrading at least one organic contaminant and a degrading bacterium B capable of degrading another organic contaminant are accumulated, is produced. The degrading bacterium A may be a PCNB-degrading bacterium, particularly a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of Nocardioides sp. PD653 and the degrading bacterium B may be a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of β-Proteobacteria CDB21.

摘要翻译： 本发明提供了一种稳定的复合微生物系统，即使在这些污染物的污染环境下也能同时分解多种有机污染物，并允许持久性有机污染物如PCNB和西马嗪的更有效分解。 产生含有多余材料的支持物，其含有多孔材料作为载体，所述多孔材料在其上堆积能够降解至少一种有机污染物的降解菌A和能够降解另一种有机污染物的降解菌B 。 降解菌A可以是PCNB降解细菌，特别是含有具有诺卡氏菌属的真菌学特征的部分或全部的降解菌的降解菌。 PD653和降解菌B可以是含有具有部分或全部β-变形杆菌CDB21的真菌学特征的降解菌的降解菌。

公开(公告)号：US07629159B2

公开(公告)日：2009-12-08

申请号：US11335686

申请日：2006-01-20

申请人： Kazuhiro Takagi ,  Naoki Harada ,  Yuuichi Yoshioka

发明人： Kazuhiro Takagi ,  Naoki Harada ,  Yuuichi Yoshioka

IPC分类号： C12N1/20 ,  C12N11/14 ,  C12N11/02 ,  C12N11/04 ,  A62D3/02

CPC分类号： C12P39/00 ,  B09C1/002 ,  B09C1/10 ,  C12N11/02 ,  C12N11/12 ,  C12R1/01 ,  C12R1/06 ,  Y10S435/822 ,  Y10S435/83

摘要： The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed enrichment of degrading bacteria, which contains a porous material provided as a support on which degrading bacteria A capable of degrading at least one organic contaminant and degrading bacteria B capable of degrading another organic contaminant are enriched, is produced. The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of β-Proteobacteria CDB21.

摘要翻译： 本发明提供了一种稳定的复合微生物系统，即使在这些污染物的污染环境下也能同时分解多种有机污染物，并允许持久性有机污染物如PCNB和西马嗪的更有效分解。 产生含有多孔材料的支持物，其含有提供作为支持物的多孔材料，所述多孔材料能够使能够降解至少一种有机污染物的降解细菌A和能够降解另一种有机污染物的降解菌体B被富集。 降解细菌A可以是PCNB降解细菌，特别是含有降解菌的降解细菌，其具有诺卡氏菌属（Nocardioides sp。）的部分或全部细菌学特征。 PD653和降解细菌B可能是含有降解细菌的降解细菌，具有部分或全部β-变形杆菌CDB21的细菌学特征。

公开(公告)号：US20060166346A1

公开(公告)日：2006-07-27

申请号：US11335686

申请日：2006-01-20

申请人： Kazuhiro Takagi ,  Naoki Harada ,  Yuuichi Yoshioka

发明人： Kazuhiro Takagi ,  Naoki Harada ,  Yuuichi Yoshioka

IPC分类号： C12N1/20 ,  B09C1/10

CPC分类号： C12P39/00 ,  B09C1/002 ,  B09C1/10 ,  C12N11/02 ,  C12N11/12 ,  C12R1/01 ,  C12R1/06 ,  Y10S435/822 ,  Y10S435/83

摘要： The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed enrichment of degrading bacteria, which contains a porous material provided as a support on which degrading bacteria A capable of degrading at least one organic contaminant and degrading bacteria B capable of degrading another organic contaminant are enriched, is produced. The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of β-Proteobacteria CDB21.

摘要翻译： 本发明提供了一种稳定的复合微生物系统，即使在这些污染物的污染环境下也能同时分解多种有机污染物并允许持久性有机污染物如PCNB和西马嗪的更有效的分解。 产生含有多孔材料的支持物，其含有提供作为支持物的多孔材料，在其上富含能够降解至少一种有机污染物的降解细菌A和降解能够降解另一有机污染物的细菌B。 降解细菌A可以是PCNB降解细菌，特别是含有降解菌的降解细菌，其具有诺卡氏菌属（Nocardioides sp。）的部分或全部细菌学特征。 PD653和降解细菌B可能是含有降解细菌的降解细菌，具有部分或全部β-变形杆菌CDB21的细菌学特征。