公开(公告)号：US06828140B2

公开(公告)日：2004-12-07

申请号：US09872339

申请日：2001-06-01

申请人： Timothy Gardner ,  James J. Collins

发明人： Timothy Gardner ,  James J. Collins

IPC分类号： C12N120

CPC分类号： C12N15/635 ,  C12N15/63

摘要： Provided are methods and compositions for regulating gene expression in a cell. The invention provides recombinant adjustable-threshold switches which contain a first inducible promoter-regulatory gene operon and a second constitutive promoter-regulatory gene operon. A threshold amount of activating agent is required to switch the inducible promoter “on” and the constitutive promoter “off”. An adjustable-threshold switch is useful to reversibly switch the expression of a gene of interest between a stable “on” state and stable “off” state in response to an activating agent at or above a threshold concentration.

摘要翻译： 提供用于调节细胞中基因表达的方法和组合物。 本发明提供了包含第一诱导型启动子调控基因操纵子和第二组成型启动子调控基因操纵子的重组可调阈值开关。 需要阈值量的活化剂来切换诱导型启动子“开”并且组成型启动子“关闭”。 可调节阈值开关对于在阈值浓度或阈值浓度以上的活化剂可逆地切换感兴趣的基因在稳定的“开”状​​态和稳定的“关闭”状态之间的表达是有用的。

公开(公告)号：US06818432B2

公开(公告)日：2004-11-16

申请号：US09819930

申请日：2001-03-29

申请人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

发明人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

IPC分类号： C12N120

CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no.2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the ptsH gene coding for component H of the phosphotransferase system, and the use of the above polynucleotides as primer or hybridisation probe.

摘要翻译： 本发明涉及含有多核苷酸序列的分离的多核苷酸，所述多核苷酸序列选自包含a）多核苷酸的多核苷酸，所述多核苷酸与编码含有SEQ ID NO：1的氨基酸序列的多肽的多核苷酸具有至少70％的同一性。 2，b）编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）多核苷酸，其包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸，以及用于增强编码磷酸转移酶系统的组分H的ptsH基因的L-氨基酸的发酵生产的方法， 以及使用上述多核苷酸作为引物或杂交探针。

公开(公告)号：US06812022B1

公开(公告)日：2004-11-02

申请号：US09889263

申请日：2001-10-16

申请人： Takemi Aonuma

发明人： Takemi Aonuma

IPC分类号： C12N120

CPC分类号： C12R1/07 ,  A23C9/1203 ,  A23C9/127 ,  A23K10/12 ,  A23K10/16 ,  A23L3/3571 ,  A23L5/28 ,  A23L11/37 ,  A23L29/065 ,  C05F11/08 ,  C12N1/20 ,  C12R1/125 ,  Y02W30/47

摘要： Microorganisms of the genus Bacillus that are capable of reducing nitrates and contain chitin and/or chitosan in their cell walls are provided. The microorganism of the invention can be used for improving soil, for treating organic waste by fermentation, for fermenting soybeans, and for reducing bitterness. The microorganism of the invention can also be used as a feed additive or a good additive. Further, the microorganism of the invention has an anti-microbial effect.

摘要翻译： 提供能够在其细胞壁中还原硝酸盐并含有壳多糖和/或壳聚糖的芽孢杆菌属的微生物。 本发明的微生物可用于改善土壤，通过发酵处理有机废物，发酵大豆和减少苦味。 本发明的微生物也可以用作饲料添加剂或良好的添加剂。 此外，本发明的微生物具有抗菌作用。

公开(公告)号：US06756222B2

公开(公告)日：2004-06-29

申请号：US09772304

申请日：2001-01-29

申请人： L. H. Mahishi ,  G. Tripathi ,  T. V. N. Ramchander ,  Shuban Kishen Rawal

发明人： L. H. Mahishi ,  G. Tripathi ,  T. V. N. Ramchander ,  Shuban Kishen Rawal

IPC分类号： C12N120

CPC分类号： C12P7/625 ,  C12N15/52 ,  C12N15/70 ,  Y02P20/582

摘要： The present invention provides a novel genetically modified Escherichia coli JM109 bearing accession number PTA 1579, containing the gene coding for poly-beta-hydroxybutyrate synthesis and a method of using this bacterium to produce poly-beta-hydroxybutyrate to the extent of 60% or more of the cell weight.

摘要翻译： 本发明提供了一种含有编码聚-β-羟基丁酸合成基因的保藏号PTA1579的新型遗传修饰的大肠杆菌JM109，以及使用该细菌生产聚-β-羟基丁酸酯至60％以上程度的方法 的细胞重量。

公开(公告)号：US06723553B2

公开(公告)日：2004-04-20

申请号：US09891711

申请日：2001-06-26

申请人： Lars Abrahmsén ,  Jonas Ekblom ,  Margareta Forsgren ,  Jan Hörling ,  Per Johansson

发明人： Lars Abrahmsén ,  Jonas Ekblom ,  Margareta Forsgren ,  Jan Hörling ,  Per Johansson

IPC分类号： C12N120

CPC分类号： C12N9/6424

摘要： The present invention relates an isolated human Site-1 Protease promoter region. The invention also relates to screening methods for agents decreasing the expression of Site-1 protease and thereby being potentially useful for the treatment of medical conditions related to obesity and/or diabetes.

摘要翻译： 本发明涉及一种分离的人类Site-1蛋白酶启动子区域。 本发明还涉及用于降低Site-1蛋白酶表达的试剂的筛选方法，因此可用于治疗与肥胖和/或糖尿病相关的医学病症。

公开(公告)号：US06703492B1

公开(公告)日：2004-03-09

申请号：US09710279

申请日：2000-11-09

申请人： William John Kimmerly

发明人： William John Kimmerly

IPC分类号： C12N120

CPC分类号： C07K14/31

摘要： S epidermidis polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides and DNA (RNA) for the treatment of infection, particularly infections arising from S epidermidis. Antagonists against the function of such polypeptides and their use as therapeutics to treat infection are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of S epidermidis nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides and polypeptides related to S epidermidis.

摘要翻译： 公开了S表皮多肽和编码这些多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这些多肽和DNA（RNA）来治疗感染，特别是表皮葡萄球菌引起的感染的方法。 还公开了抗这种多肽的功能的拮抗剂及其作为治疗剂治疗感染的用途。 还公开了用于检测与宿主中表皮葡萄球菌核酸序列和多肽存在相关的疾病的诊断测定法。 还公开了用于检测与S表皮相关的多核苷酸和多肽的诊断测定。

公开(公告)号：US06689357B2

公开(公告)日：2004-02-10

申请号：US09985846

申请日：2001-11-06

申请人： Lester Earl Casida, Jr. ,  Joseph Oliver Falkinham, III ,  Cody Christopher Cain

发明人： Lester Earl Casida, Jr. ,  Joseph Oliver Falkinham, III ,  Cody Christopher Cain

IPC分类号： C12N120

CPC分类号： C12N9/52 ,  A01N63/00 ,  C12P1/04 ,  C12R1/01 ,  Y10S435/874

摘要： A novel predator bacterium Burkholderia casidae is disclosed. The invention is directed to the isolation and use of Burkholderia casidae to control microbial diseases of plants. The genetic, biochemical and physiological characteristics of Burkholderia casidae are described. Biocontrol compositions comprising Burkholderia casidae, and antimicrobial compounds and antimicrobial preparations prepared from Burkholderia casidae are also disclosed, as are methods for accomplishing all of the foregoing.

摘要翻译： 公开了一种新颖的捕食性细菌伯克霍尔德氏菌（Burkholderia casidae）。 本发明涉及分离和使用伯克霍尔德杆菌来控制植物的微生物疾病。 描述了伯克霍尔德氏菌属的遗传，生化和生理特征。 还公开了包含伯克霍尔德氏菌属的生物控制组合物，以及由伯克霍尔德氏菌（Bkholderholder casidae）制备的抗微生物化合物和抗微生物制剂，以及完成上述所有方法。

公开(公告)号：US06638755B1

公开(公告)日：2003-10-28

申请号：US09926551

申请日：2001-11-19

申请人： Shingo Mizuochi ,  Hidemasa Kodaka ,  Hideaki Shibata

发明人： Shingo Mizuochi ,  Hidemasa Kodaka ,  Hideaki Shibata

IPC分类号： C12N120

CPC分类号： C12N1/20 ,  C12N1/22 ,  C12Q1/045

摘要： A simple culture medium produced by impregnating a fibrous water-absorbent sheet with a suspension formed by suspending in an alcohol (a) an adhesive (0.01-0.4 wt. %) which is soluble in both water and alcohol, (b) a gelling agent which is soluble in water and insoluble in alcohol, and (c) a bacterial nutritive ingredient, the fibrous water-absorbent sheet having a mesh larger than the particle size of the gelling agent and being placed on a waterproof flat plate, and by drying the resultant sheet while suppressing rapid evaporation of the alcohol, to thereby cause the water-absorbent sheet to adhere onto the waterproof flat plate; and a method for producing the medium.

摘要翻译： 通过将悬浮在醇（a）可溶于水和醇的粘合剂（0.01-0.4重量％）悬浮而形成的悬浮液浸渍纤维状吸水片而制备的简单培养基，（b）胶凝剂 其可溶于水并且不溶于醇，和（c）细菌营养成分，所述纤维性吸水片具有比所述胶凝剂的粒径大的网状物，并且被放置在防水平板上，并通过干燥 同时抑制醇的快速蒸发，从而使吸水片粘附到防水平板上; 和介质的制造方法。

公开(公告)号：US06627188B1

公开(公告)日：2003-09-30

申请号：US09702222

申请日：2000-10-31

申请人： Arthur Hinton, Jr. ,  Kimberly D. Ingram ,  Richard Jeffery Buhr

发明人： Arthur Hinton, Jr. ,  Kimberly D. Ingram ,  Richard Jeffery Buhr

IPC分类号： C12N120

CPC分类号： A23K50/75 ,  A23K20/147 ,  A23K20/163 ,  A23K40/00

摘要： A method for reducing bacterial enteropathogens in fowl subjected to feed withdrawal, involving withdrawing feed from fowl and orally administering to the fowl an effective amount of a cocktail sufficient to reduce bacterial enteropathogens in the crop of the fowl, wherein the cocktail contains contains water, a carbohydrate (e.g., glucose, sucrose), and optionally a protein source, a vitamin source, an organic acid, an emulsifier, a mineral source, and mixtures thereof.

摘要翻译： 一种用于在进行饲料撤出的家禽中减少细菌肠病原体的方法，包括从家禽中取出饲料并口服给予家禽有效量的足以减少家禽作物中的细菌性肠病的鸡尾酒，其中鸡尾酒含有水， 碳水化合物（例如葡萄糖，蔗糖）和任选的蛋白质来源，维生素源，有机酸，乳化剂，矿物源及其混合物。

公开(公告)号：US06617149B2

公开(公告)日：2003-09-09

申请号：US10100261

申请日：2002-03-18

申请人： Lawrence Restaino

发明人： Lawrence Restaino

IPC分类号： C12N120

CPC分类号： C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52 ,  G01N2333/245

摘要： An isolation plating medium for use in processes for the presumptive identification of Escherichia coli 0157:H7 from a sample that also contains other strains of Escherichia coli. The plating medium comprises at least one carbohydrate that Escherichia coli 0157:H7 is incapable of fermenting, but other strains of Escherichia coli do ferment said carbohydrate, a pH indicator dye that changes the color of the plating medium to a first color when the pH of the medium changes, a chromogenic substrate that reacts to beta-galactosidase to form a precipitate in the plating medium of a second color which contrasts with the first color. Both Escherichia coli 0157:H7 and the other strains of Escherichia coli are beta-galactosidase producers during metabolism, whereby a microorganism which ferments the carbohydrate but does not produce beta-galactosidase will produce colonies in the plating medium of the first color, Escherichia coli 0157:H7 which does not ferment the carbohydrate but produces beta-galactosidase will produce colonies in the plating medium of the second color, and the other strains of Escherichia coli which ferment the carbohydrate medium and produce beta-galactosidase will produce colonies in the plating medium of a third color which is the color that results from the mixing of the first and second colors, and a sufficient mass of an agent to solidify the mixture. The invention also includes processes of using the plating medium to identify and enumerate Escherichia coli 0157:H7 and other microorganisms.