公开(公告)号：US20210115415A1

公开(公告)日：2021-04-22

申请号：US17114016

申请日：2020-12-07

Applicant: 10X Genomics, Inc.

Inventor： Josephine Lee ,  Samuel Marrs ,  Geoffrey McDermott ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Joseph Francis Shuga ,  Jessica Michele Terry ,  Solongo B. Ziraldo

IPC: C12N9/12 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6844

Abstract: Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.

公开(公告)号：US11584954B2

公开(公告)日：2023-02-21

申请号：US16855177

申请日：2020-04-22

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila Belhocine ,  Josephine Lee ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Andrew D. Price ,  Jerald Sapida ,  David Sukovich ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12Q1/6806

Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

公开(公告)号：US20180312822A1

公开(公告)日：2018-11-01

申请号：US15975516

申请日：2018-05-09

Applicant: 10X Genomics, Inc.

Inventor： Josephine Lee ,  Samuel Marrs ,  Geoffrey McDermott ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Joseph Francis Shuga ,  Jessica Michele Terry ,  Solongo B. Ziraldo

IPC: C12N9/12 ,  C12N15/10 ,  C12Q1/6806

Abstract: Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.