公开(公告)号：US12241060B2

公开(公告)日：2025-03-04

申请号：US18178367

申请日：2023-03-03

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6804 ,  C12N15/10

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US20240301489A1

公开(公告)日：2024-09-12

申请号：US18605260

申请日：2024-03-14

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Joseph Francis Shuga ,  Patrick Roelli ,  Denise Cheung ,  Alexander Hermes ,  Yifeng Yin ,  Naishitha Anaparthy ,  Ryo Hatori ,  Marlon Stoeckius ,  Rapolas Spalinskas ,  Anna-Maria Katsori ,  Cedric Uytingco ,  Mesruh Turkekul ,  David Sukovich ,  Christina Galonska ,  Aleksandra Jurek ,  Octavian Marian Bloju

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6837

Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

公开(公告)号：US20230416808A1

公开(公告)日：2023-12-28

申请号：US18243582

申请日：2023-09-07

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Felice Alessio Bava ,  Augusto Manuel Tentori ,  Hanyoup Kim ,  Amanda Janesick

IPC: C12Q1/6841

CPC classification number: C12Q1/6841

Abstract: Provided herein are methods, compositions, and systems for improved in situ detection of analytes and spatial analysis using, e.g., a sequencing readout.

公开(公告)号：US20230295699A1

公开(公告)日：2023-09-21

申请号：US18311605

申请日：2023-05-03

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US12195790B2

公开(公告)日：2025-01-14

申请号：US18243582

申请日：2023-09-07

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Felice Alessio Bava ,  Augusto Manuel Tentori ,  Hanyoup Kim ,  Amanda Janesick

IPC: C12Q1/68 ,  C12Q1/6841

Abstract: Provided herein are methods, compositions, and systems for improved in situ detection of analytes and spatial analysis using, e.g., a sequencing readout.

公开(公告)号：US20240417784A1

公开(公告)日：2024-12-19

申请号：US18815241

申请日：2024-08-26

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US12071655B2

公开(公告)日：2024-08-27

申请号：US18311605

申请日：2023-05-03

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US20240218427A1

公开(公告)日：2024-07-04

申请号：US18399109

申请日：2023-12-28

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hardeep Pal Singh ,  Lauren Gutgesell ,  Ace George Santiago ,  Benton Veire ,  Anuj Patel

IPC: C12Q1/6823 ,  G01N33/58 ,  G01N33/68

CPC classification number: C12Q1/6823 ,  G01N33/58 ,  G01N33/6857 ,  G01N2458/10

Abstract: Provided herein are methods for spatial analysis of proteins and/or nucleic acids by capturing a capture handle sequence and/or a connected probe to a capture domain of a capture probe on a spatial array. In some instances, a reagent medium including a monovalent or divalent salt, ethylene carbonate, and/or glycerol is used in the disclosed methods.

公开(公告)号：US20230203478A1

公开(公告)日：2023-06-29

申请号：US18178367

申请日：2023-03-03

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US11680260B2

公开(公告)日：2023-06-20

申请号：US17976422

申请日：2022-10-28

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori ,  Lauren Gutgesell ,  Janine Hensel ,  Seayar Mohabbat

IPC: C12Q1/6837 ,  C12N15/10

CPC classification number: C12N15/1065

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.