公开(公告)号：US20230236175A1

公开(公告)日：2023-07-27

申请号：US17820560

申请日：2022-08-17

Applicant: 10x Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Jason Bell ,  Jerald Sapida ,  Katrina Sullivan-Bibee

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

CPC classification number: G01N33/532 ,  C07K14/70539 ,  C12N15/85 ,  G01N33/54306 ,  G01N33/548 ,  C12N15/1037 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/505 ,  G01N33/5308 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/5304 ,  G01N33/5306 ,  C12N15/1055 ,  C12N15/1065 ,  G01N33/5032 ,  G01N33/54366 ,  G01N33/56977 ,  C12Q2537/164 ,  C12Q2565/1015 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12N2310/20 ,  C40B70/00

Abstract: The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

公开(公告)号：US20240417784A1

公开(公告)日：2024-12-19

申请号：US18815241

申请日：2024-08-26

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US12071655B2

公开(公告)日：2024-08-27

申请号：US18311605

申请日：2023-05-03

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US20230295699A1

公开(公告)日：2023-09-21

申请号：US18311605

申请日：2023-05-03

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US11467153B2

公开(公告)日：2022-10-11

申请号：US17393107

申请日：2021-08-03

Applicant: 10x Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Jason Bell ,  Jerald Sapida ,  Katrina Sullivan-Bibee

IPC: G01N33/532 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6881 ,  C40B50/06 ,  C07K14/74 ,  C12Q1/6804 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12N15/11 ,  G01N33/569 ,  C40B70/00 ,  C40B30/04

Abstract: The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

公开(公告)号：US11584954B2

公开(公告)日：2023-02-21

申请号：US16855177

申请日：2020-04-22

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila Belhocine ,  Josephine Lee ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Andrew D. Price ,  Jerald Sapida ,  David Sukovich ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12Q1/6806

Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

公开(公告)号：US20210364502A1

公开(公告)日：2021-11-25

申请号：US17393107

申请日：2021-08-03

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Jason Bell ,  Jerald Sapida ,  Katrina Sullivan-Bibee

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

Abstract: The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

公开(公告)号：US20200291454A1

公开(公告)日：2020-09-17

申请号：US16789287

申请日：2020-02-12

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila Belhocine ,  Jason Bell ,  Jerald Sapida ,  Katrina Sullivan-Bibee

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6869 ,  C12Q1/6844

Abstract: The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.