公开(公告)号：US12138628B2

公开(公告)日：2024-11-12

申请号：US17397146

申请日：2021-08-09

Applicant: 10x Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Tobias Daniel Wheeler

IPC: B01L3/00 ,  G01N1/28 ,  G01N33/487

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

公开(公告)号：US12110549B2

公开(公告)日：2024-10-08

申请号：US17822117

申请日：2022-08-24

Applicant: 10x Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20190367966A1

公开(公告)日：2019-12-05

申请号：US16375102

申请日：2019-04-04

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC: C12Q1/6806 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6804 ,  G01N33/50 ,  C12N15/10

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.

公开(公告)号：US20190338353A1

公开(公告)日：2019-11-07

申请号：US16426762

申请日：2019-05-30

Applicant: 10X GENOMICS, INC.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/683 ,  C12Q1/6806 ,  C12Q1/6804

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20180282803A1

公开(公告)日：2018-10-04

申请号：US15933299

申请日：2018-03-22

Applicant: 10X GENOMICS, INC.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20180223342A1

公开(公告)日：2018-08-09

申请号：US15896543

申请日：2018-02-14

Applicant: 10X Genomics, Inc.

Inventor： Tobias Daniel Wheeler ,  Rajiv Bharadwaj ,  Katherine Pfeiffer

IPC: C12Q1/6811 ,  C12Q1/6806 ,  C12N15/10

CPC classification number: C12N15/1096 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/111 ,  C12N2310/20 ,  C12Q1/686 ,  C12Q2521/531 ,  C12Q2521/539 ,  C12Q1/6806 ,  C12Q2521/301 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/179

Abstract: The present disclosure provides methods and systems for nucleic acid processing. A method for preparing a sequencing set may include providing a template nucleic acid and amplifying the template nucleic acid to provide a complementary nucleic acid. Next, the complementary nucleic acid may be fragmented and barcoded to produce a first set of barcoded fragments comprising a plurality of first barcoded fragments. Next, the plurality of first barcoded fragments may be fragmented to yield a second set of barcoded fragments comprising a plurality of second barcoded fragments.

公开(公告)号：US12269036B2

公开(公告)日：2025-04-08

申请号：US17458757

申请日：2021-08-27

Applicant: 10x Genomics, Inc.

Inventor： Ivan Akhremichev ,  Rajiv Bharadwaj ,  Lynna Chen ,  Mohammad Rahimi Lenji ,  Alireza Salmanzadeh ,  Martin Sauzade ,  Tobias Daniel Wheeler ,  Yiran Zhang

IPC: B01L3/00 ,  B01F23/00 ,  B01F23/41 ,  B01F25/314 ,  B01F33/3011 ,  B01F101/23 ,  B23Q17/24 ,  C07K14/705 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/686 ,  G01N15/1492 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N27/414 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/74 ,  H10K10/46 ,  H10K85/00 ,  H10K85/20 ,  G01N15/10 ,  G01N15/14 ,  G01N15/149

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US11833515B2

公开(公告)日：2023-12-05

申请号：US16859060

申请日：2020-04-27

Applicant: 10x Genomics, Inc.

Inventor： Tobias Daniel Wheeler ,  Bill Kengli Lin ,  Mohammad Rahimi Lenji ,  Anthony Makarewicz ,  Rajiv Bharadwaj

IPC: B01L3/00 ,  C12Q1/6869

CPC classification number: B01L3/502784 ,  B01L3/502761 ,  C12Q1/6869 ,  B01L2200/06 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/0861

Abstract: Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

公开(公告)号：US20230203577A1

公开(公告)日：2023-06-29

申请号：US17822117

申请日：2022-08-24

Applicant: 10x Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806 ,  C12Q2563/179 ,  C12Q2537/143 ,  C12Q2525/191

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20230144760A1

公开(公告)日：2023-05-11

申请号：US17819397

申请日：2022-08-12

Applicant: 10x Genomics, Inc.

Inventor： Jessica Michele Terry ,  Tobias Daniel Wheeler

IPC: C12Q1/6869 ,  C12Q1/6834

CPC classification number: C12Q1/6869 ,  C12Q1/6834 ,  C12Q2565/102 ,  C12Q2600/158

Abstract: Provided herein are methods, compositions, and systems for multiplexed analysis of individual cells or cell populations. Cells encapsulated in beads and/or biomolecules are sequentially co-partitioned, allowing for analysis of two different types of biomolecules (e.g., RNA and DNA). The present invention leverages different polymer dissociation mechanisms, accompanied with barcoding of biomolecules (e.g., nucleic acid molecules) for multiplexed measurements in single cells. Sequential co-partitioning and barcode technology enables identification and quantitation of DNA and RNA from single cells.