公开(公告)号：US20200290049A1

公开(公告)日：2020-09-17

申请号：US16887024

申请日：2020-05-29

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Tobias Daniel WHEELER ,  Kevin NESS ,  Benjamin HINDSON

IPC: B01L3/00 ,  B01F13/00 ,  B01J2/06 ,  C12Q1/6844 ,  B01F3/08

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

公开(公告)号：US20240218437A1

公开(公告)日：2024-07-04

申请号：US18542038

申请日：2023-12-15

Applicant: 10x Genomics, Inc.

Inventor： Zahra Kamila BELHOCINE ,  Zachary W. BENT ,  Rajiv BHARADWAJ ,  Alexander GAGNON ,  Qiang GONG ,  Ashley HAYES ,  Benjamin HINDSON ,  Yuwei LI ,  Benjamin PRUITT ,  Daniel P. RIORDAN ,  Hiroshi SASAKI ,  Weiyi TANG

IPC: C12Q1/6844 ,  C12Q1/6816

CPC classification number: C12Q1/6846 ,  C12Q1/6816

Abstract: The present disclosure relates in some aspects to methods and compositions for assessing system performance for in situ analyte detection. In some aspects, performance of an individual instrument can be assessed, or the performance of two or more instruments can be assessed and optionally compared. In some aspects, disclosed herein is a method comprising using rolling circle amplification products (RCPs) deposited on a cell-free and tissue-free quality control (QC) slide to assess performance of instrument workflow, where an instrument is used to decode signals associated with the RCPs on the QC slide. Quality metrics associated with the decoding (e.g., a percentage of RCPs successfully decoded to genes) can be used to qualify a system comprising the instrument and reagents for in situ analyte detection in cells or tissue samples, e.g., using in situ probe hybridization or in situ sequencing performed on the instrument.

公开(公告)号：US20220097045A1

公开(公告)日：2022-03-31

申请号：US17471603

申请日：2021-09-10

Applicant: 10x Genomics, Inc.

Inventor： Donald A. MASQUELIER ,  Benjamin HINDSON ,  Kevin NESS ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  F16K99/00 ,  B01L9/00 ,  C12Q1/6874

Abstract: An integrated system for processing and preparing samples for analysis may include a microfluidic device including a plurality of parallel channel networks for partitioning the samples including various fluids, and connected to a plurality of inlet and outlet reservoirs, at least a portion of the fluids comprising reagents, a holder including a closeable lid hingedly coupled thereto, in which in a closed configuration, the lid secures the microfluidic device in the holder, and in an open configuration, the lid is a stand orienting the microfluidic device at a desired angle to facilitate recovery of partitions or droplets from the partitioned samples generated within the microfluidic device, and an instrument configured to receive the holder and apply a pressure differential between the plurality of inlet and outlet reservoirs to drive fluid movement within the channel networks.

公开(公告)号：US20210129148A1

公开(公告)日：2021-05-06

申请号：US17145858

申请日：2021-01-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin NESS ,  Debkishore MITRA ,  Donald A. MASQUELIER ,  Anthony MAKAREWICZ ,  Christopher HINDSON ,  Benjamin HINDSON ,  Serge SAXONOV

IPC: B01L3/00 ,  B01L3/02

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.