公开(公告)号：US20210269852A1

公开(公告)日：2021-09-02

申请号：US17064508

申请日：2020-10-06

Applicant: 10X Genomics, Inc.

Inventor： Mirna JAROSZ ,  Michael SCHNALL-LEVIN ,  Serge SAXONOV ,  Benjamin J. HINDSON ,  Xinying ZHENG

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6837

Abstract: The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.

公开(公告)号：US20200216897A1

公开(公告)日：2020-07-09

申请号：US16773750

申请日：2020-01-27

Applicant: 10X GENOMICS, INC.

Inventor： Josephine HARADA ,  Tarjei Sigurd MIKKELSEN ,  Katherine PFEIFFER ,  Serge SAXONOV ,  John R. STUELPNAGEL

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20230073186A1

公开(公告)日：2023-03-09

申请号：US17943064

申请日：2022-09-12

Applicant: 10X GENOMICS, INC.

Inventor： Xinying ZHENG ,  Serge SAXONOV ,  Michael SCHNALL-LEVIN ,  Kevin NESS ,  Rajiv BHARADWAJ

IPC: C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

Abstract: The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.

公开(公告)号：US20220106641A1

公开(公告)日：2022-04-07

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip BELGRADER ,  Zachary BENT ,  Rajiv BHARADWAJ ,  Vijay Kumar Sreenivasa GOPALAN ,  Josephine HARADA ,  Christopher HINDSON ,  Mohammad Rahimi LENJI ,  Michael Ybarra LUCERO ,  Geoffrey McDERMOTT ,  Elliott MEER ,  Tarjei Sigurd MIKKELSEN ,  Christopher Joachim O'KEEFFE ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Paul RYVKIN ,  Serge SAXONOV ,  John R. STUELPNAGEL ,  Jessica Michele TERRY ,  Tobias Daniel WHEELER ,  Indira WU ,  Solongo Batjargal ZIRALDO ,  Stephane Claude BOUTET ,  Sarah TAYLOR ,  Niranjan SRINIVAS

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20240167079A1

公开(公告)日：2024-05-23

申请号：US18520514

申请日：2023-11-27

Applicant: 10X GENOMICS, INC.

Inventor： Xinying ZHENG ,  Serge SAXONOV ,  Michael SCHNALL-LEVIN ,  Kevin NESS ,  Rajiv BHARADWAJ

IPC: C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

CPC classification number: C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

Abstract: The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.

公开(公告)号：US20240035066A1

公开(公告)日：2024-02-01

申请号：US18346011

申请日：2023-06-30

Applicant: 10X Genomics, Inc.

Inventor： Mirna JAROSZ ,  Michael SCHNALL-LEVIN ,  Serge SAXONOV ,  Benjamin J. HINDSON ,  Xinying ZHENG

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6837

CPC classification number: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6837

Abstract: The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.

公开(公告)号：US20210129148A1

公开(公告)日：2021-05-06

申请号：US17145858

申请日：2021-01-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin NESS ,  Debkishore MITRA ,  Donald A. MASQUELIER ,  Anthony MAKAREWICZ ,  Christopher HINDSON ,  Benjamin HINDSON ,  Serge SAXONOV

IPC: B01L3/00 ,  B01L3/02

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US20210079466A1

公开(公告)日：2021-03-18

申请号：US17033218

申请日：2020-09-25

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Serge SAXONOV

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12P19/34 ,  C12N15/10

Abstract: Provided herein are methods and systems for processing a nucleic acid molecule from a biological particle (e.g., cell). A plurality of partitions (e.g., droplets) may be generated such that partitions of the plurality of partitions each include a biological particle (e.g., cell) comprising the nucleic acid molecule and a particle (e.g., bead). The partitions can be processed (e.g., imaged) to obtain one or more physical and/or optical properties of their respective biological particles. The nucleic acid molecules included in the partitions can be barcoded and sequenced (e.g., using nucleic acid barcode molecules coupled to the particles of the partitions) to generate nucleic acid sequences of the nucleic acid molecules. The nucleic acid sequences can be electronically associated with the one or more optical properties of the biological particles.

公开(公告)号：US20190153532A1

公开(公告)日：2019-05-23

申请号：US16228261

申请日：2018-12-20

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Serge SAXONOV

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12N15/10 ,  C12P19/34

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/1089 ,  C12N15/1093 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2563/179 ,  C12Q2563/159

Abstract: Provided herein are methods and systems for processing a nucleic acid molecule from a biological particle (e.g., cell). A plurality of partitions (e.g., droplets) may be generated such that partitions of the plurality of partitions each include a biological particle (e.g., cell) comprising the nucleic acid molecule and a particle (e.g., bead). The partitions can be processed (e.g., imaged) to obtain one or more physical and/or optical properties of their respective biological particles. The nucleic acid molecules included in the partitions can be barcoded and sequenced (e.g., using nucleic acid barcode molecules coupled to the particles of the partitions) to generate nucleic acid sequences of the nucleic acid molecules. The nucleic acid sequences can be electronically associated with the one or more optical properties of the biological particles.