公开(公告)号：US20210032678A1

公开(公告)日：2021-02-04

申请号：US16913084

申请日：2020-06-26

Applicant: 10X Genomics, Inc.

Inventor： Phillip BELGRADER ,  Donald A. MASQUELIER

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6869

Abstract: The disclosure provides methods for separating and/or purifying one or more molecules released from one or more fluid compartments or partitions, such as one or more droplets. Molecules can be released from a fluid compartment(s) and bound to supports that can be isolated via any suitable method, including example methods described herein. The disclosure also provides devices that can aid in isolating supports bound to molecules.

公开(公告)号：US20240326052A1

公开(公告)日：2024-10-03

申请号：US18738471

申请日：2024-06-10

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin Ness ,  Debkishore Mitra ,  Donald A. MASQUELIER ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  B01L3/02 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N2035/00237 ,  G01N35/085 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US20220097045A1

公开(公告)日：2022-03-31

申请号：US17471603

申请日：2021-09-10

Applicant: 10x Genomics, Inc.

Inventor： Donald A. MASQUELIER ,  Benjamin HINDSON ,  Kevin NESS ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  F16K99/00 ,  B01L9/00 ,  C12Q1/6874

Abstract: An integrated system for processing and preparing samples for analysis may include a microfluidic device including a plurality of parallel channel networks for partitioning the samples including various fluids, and connected to a plurality of inlet and outlet reservoirs, at least a portion of the fluids comprising reagents, a holder including a closeable lid hingedly coupled thereto, in which in a closed configuration, the lid secures the microfluidic device in the holder, and in an open configuration, the lid is a stand orienting the microfluidic device at a desired angle to facilitate recovery of partitions or droplets from the partitioned samples generated within the microfluidic device, and an instrument configured to receive the holder and apply a pressure differential between the plurality of inlet and outlet reservoirs to drive fluid movement within the channel networks.

公开(公告)号：US20210129148A1

公开(公告)日：2021-05-06

申请号：US17145858

申请日：2021-01-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin NESS ,  Debkishore MITRA ,  Donald A. MASQUELIER ,  Anthony MAKAREWICZ ,  Christopher HINDSON ,  Benjamin HINDSON ,  Serge SAXONOV

IPC: B01L3/00 ,  B01L3/02

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.