公开(公告)号：US20220106641A1

公开(公告)日：2022-04-07

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip BELGRADER ,  Zachary BENT ,  Rajiv BHARADWAJ ,  Vijay Kumar Sreenivasa GOPALAN ,  Josephine HARADA ,  Christopher HINDSON ,  Mohammad Rahimi LENJI ,  Michael Ybarra LUCERO ,  Geoffrey McDERMOTT ,  Elliott MEER ,  Tarjei Sigurd MIKKELSEN ,  Christopher Joachim O'KEEFFE ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Paul RYVKIN ,  Serge SAXONOV ,  John R. STUELPNAGEL ,  Jessica Michele TERRY ,  Tobias Daniel WHEELER ,  Indira WU ,  Solongo Batjargal ZIRALDO ,  Stephane Claude BOUTET ,  Sarah TAYLOR ,  Niranjan SRINIVAS

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20220348901A1

公开(公告)日：2022-11-03

申请号：US17551761

申请日：2021-12-15

Applicant: 10x Genomics, Inc.

Inventor： Stephane Claude BOUTET ,  Alireza SALMANZADEH ,  Michael GIBBONS

IPC: C12N15/10 ,  B01L3/00

Abstract: Devices, kits, and their methods of use for lysing and/or purifying biological particles, e.g., nuclei are provided. One or more thixotropic layers can be employed in a vessel to purify biological particles. A device with sharp features may be employed to lyse biological particles or the contents thereof.

公开(公告)号：US20220236258A1

公开(公告)日：2022-07-28

申请号：US17716134

申请日：2022-04-08

Applicant: 10x Genomics, Inc.

Inventor： Geoffrey MCDERMOTT ,  Sarah TAYLOR ,  Stephane Claude BOUTET ,  Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON

IPC: G01N33/543

Abstract: Provided herein are methods and systems for measuring secreted cytokines or other analytes from single cells. The methods disclosed herein include the use of analyte-specific and/or barcoded binding agents (e.g., antibodies) and beads in partitions (e.g., droplets or wells) to measure such analytes on a single cell basis. Further described herein are methods comprising the use of hydrogel-encapsulated cells (e.g., cell beads) to measure secreted and/or cellular analytes from single cells.