公开(公告)号：US20210205811A1

公开(公告)日：2021-07-08

申请号：US17144575

申请日：2021-01-08

Applicant: 10X Genomics, Inc.

Inventor： Alireza SALMANZADEH ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  G01N29/22

Abstract: Devices, systems, and their methods of use, for sorting droplets or particles are provided. A source of acoustic energy can be employed to sort droplets or particles of a desired and predictable property.

公开(公告)号：US20220348901A1

公开(公告)日：2022-11-03

申请号：US17551761

申请日：2021-12-15

Applicant: 10x Genomics, Inc.

Inventor： Stephane Claude BOUTET ,  Alireza SALMANZADEH ,  Michael GIBBONS

IPC: C12N15/10 ,  B01L3/00

Abstract: Devices, kits, and their methods of use for lysing and/or purifying biological particles, e.g., nuclei are provided. One or more thixotropic layers can be employed in a vessel to purify biological particles. A device with sharp features may be employed to lyse biological particles or the contents thereof.

公开(公告)号：US20220280933A1

公开(公告)日：2022-09-08

申请号：US17751267

申请日：2022-05-23

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Hanyoup KIM ,  Bill Kengli LIN ,  Marissa PENNELL ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER

IPC: B01L3/02 ,  B01L3/00

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. The devices, systems, and methods may include transporting a first liquid through an outlet of a channel and causing relative motion of the outlet and an interface of a second liquid to produce droplets of the first liquid in the second liquid. The devices, systems, and methods may also include illuminating a portion of the liquid as the liquid exits from an outlet. The invention also provides methods, devices, and systems for changing the size of a droplet and for eliminating a droplet from a plurality of droplets.

公开(公告)号：US20240301398A1

公开(公告)日：2024-09-12

申请号：US18606845

申请日：2024-03-15

Applicant: 10x Genomics, Inc.

Inventor： Alireza SALMANZADEH ,  Dagmar WALTER

IPC: C12N15/10 ,  B03C1/28

CPC classification number: C12N15/1013 ,  B03C1/28 ,  B03C2201/18 ,  B03C2201/26

Abstract: Systems, and their methods of use, for sorting or separating magnetic particles are provided. A system having a magnetic module with features that mate with voids in a flow module exerts a magnetic field on magnetic particles to separate particles.

公开(公告)号：US20220275444A1

公开(公告)日：2022-09-01

申请号：US17680850

申请日：2022-02-25

Applicant: 10X GENOMICS, INC.

Inventor： Alireza SALMANZADEH

IPC: C12Q1/6874 ,  C12Q1/6841 ,  C12Q1/6837

Abstract: The present disclosure relates generally to devices and methods useful for generating high-density arrays of target features (e.g., beads) by a permanent magnet and a substrate comprising an array of trapping regions.

公开(公告)号：US20220080424A1

公开(公告)日：2022-03-17

申请号：US17357617

申请日：2021-06-24

Applicant: 10X Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Francis CUI ,  Rachel GERVER ,  Mohammad Rahimi LENJI ,  Bill Kengli LIN ,  Anthony MAKAREWICZ ,  Martin SAUZADE ,  Astha TANNA ,  Fernandino VALDECANAS ,  Tobias Daniel WHEELER ,  Yiran ZHANG ,  Alireza SALMANZADEH

IPC: B01L3/00 ,  B01L7/00

Abstract: Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.

公开(公告)号：US20210387195A1

公开(公告)日：2021-12-16

申请号：US17458757

申请日：2021-08-27

Applicant: 10x Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Mohammad Rahimi LENJI ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER ,  Yiran ZHANG

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08 ,  B01F5/04

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20210293693A1

公开(公告)日：2021-09-23

申请号：US17343281

申请日：2021-06-09

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Jianying CAO ,  Mohammad Rahimi LENJI ,  Anthony MAKAREWICZ ,  Alireza SALMANZADEH

IPC: G01N15/14 ,  B01L3/00

Abstract: Devices, systems, and their methods of use, for detecting and/or sorting droplets or particles are provided. One or more sorters can be selected to sort droplets or particles of a desired and predictable property or droplet size. The invention involves detection of droplets or particles from a droplet or particle source through a detection region.

公开(公告)号：US20210053053A1

公开(公告)日：2021-02-25

申请号：US16998832

申请日：2020-08-20

Applicant: 10X Genomics, Inc.

Inventor： Alireza SALMANZADEH ,  Rajiv BHARADWAJ

IPC: B01L3/00

Abstract: Devices and systems employing surface acoustic waves and their methods of use, for detecting the contents of and mixing fluids are provided. Devices and systems of the invention include a piezoelectric layer on an elastic base layer and a fluidic layer including a channel.