公开(公告)号：US20250147036A1

公开(公告)日：2025-05-08

申请号：US18708833

申请日：2022-11-09

Applicant: 10x Genomics, Inc.

Inventor： Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON ,  Geoffrey P. MCDERMOTT ,  David Michael PATTERSON ,  Camilla ENGBLOM ,  Kim THRANE ,  Jeffrey MOLD ,  Jonas FRISEN ,  Joakim LUNDBERG ,  Qirong LIN ,  Caroline Julie GALLANT ,  Marlon STOECKIUS ,  Katherine PFEIFFER ,  Zachary BENT ,  Christina GALONSKA ,  Ariel ROYALL

IPC: G01N33/68 ,  C12N15/10

Abstract: Provided herein are methods for identification of antigen binding molecules such as antibodies from a sample by exposing the antigen binding molecules to an antigen conjugated to an oligonucleotide.

公开(公告)号：US20210262018A1

公开(公告)日：2021-08-26

申请号：US17180325

申请日：2021-02-19

Applicant: 10x Genomics, Inc.

Inventor： Felice Alessio BAVA ,  Zachary BENT

IPC: C12Q1/6837 ,  C12Q1/6844

Abstract: In some embodiments, provided herein is an integrated assay of a biological sample comprising an in situ assay module and a spatial assay module. The in situ assay comprises analyzing binding between nucleic acid probes and a first analyte at a spatial location of the biological sample. In some embodiments, the method further comprises providing conditions to allow spatially barcoded capture agents to capture a second analyte for analysis in the spatial assay module.

公开(公告)号：US20220106641A1

公开(公告)日：2022-04-07

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip BELGRADER ,  Zachary BENT ,  Rajiv BHARADWAJ ,  Vijay Kumar Sreenivasa GOPALAN ,  Josephine HARADA ,  Christopher HINDSON ,  Mohammad Rahimi LENJI ,  Michael Ybarra LUCERO ,  Geoffrey McDERMOTT ,  Elliott MEER ,  Tarjei Sigurd MIKKELSEN ,  Christopher Joachim O'KEEFFE ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Paul RYVKIN ,  Serge SAXONOV ,  John R. STUELPNAGEL ,  Jessica Michele TERRY ,  Tobias Daniel WHEELER ,  Indira WU ,  Solongo Batjargal ZIRALDO ,  Stephane Claude BOUTET ,  Sarah TAYLOR ,  Niranjan SRINIVAS

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.