公开(公告)号：US20210387195A1

公开(公告)日：2021-12-16

申请号：US17458757

申请日：2021-08-27

Applicant: 10x Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Mohammad Rahimi LENJI ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER ,  Yiran ZHANG

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08 ,  B01F5/04

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20210293693A1

公开(公告)日：2021-09-23

申请号：US17343281

申请日：2021-06-09

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Jianying CAO ,  Mohammad Rahimi LENJI ,  Anthony MAKAREWICZ ,  Alireza SALMANZADEH

IPC: G01N15/14 ,  B01L3/00

Abstract: Devices, systems, and their methods of use, for detecting and/or sorting droplets or particles are provided. One or more sorters can be selected to sort droplets or particles of a desired and predictable property or droplet size. The invention involves detection of droplets or particles from a droplet or particle source through a detection region.

公开(公告)号：US20220106641A1

公开(公告)日：2022-04-07

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip BELGRADER ,  Zachary BENT ,  Rajiv BHARADWAJ ,  Vijay Kumar Sreenivasa GOPALAN ,  Josephine HARADA ,  Christopher HINDSON ,  Mohammad Rahimi LENJI ,  Michael Ybarra LUCERO ,  Geoffrey McDERMOTT ,  Elliott MEER ,  Tarjei Sigurd MIKKELSEN ,  Christopher Joachim O'KEEFFE ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Paul RYVKIN ,  Serge SAXONOV ,  John R. STUELPNAGEL ,  Jessica Michele TERRY ,  Tobias Daniel WHEELER ,  Indira WU ,  Solongo Batjargal ZIRALDO ,  Stephane Claude BOUTET ,  Sarah TAYLOR ,  Niranjan SRINIVAS

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20220080424A1

公开(公告)日：2022-03-17

申请号：US17357617

申请日：2021-06-24

Applicant: 10X Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Francis CUI ,  Rachel GERVER ,  Mohammad Rahimi LENJI ,  Bill Kengli LIN ,  Anthony MAKAREWICZ ,  Martin SAUZADE ,  Astha TANNA ,  Fernandino VALDECANAS ,  Tobias Daniel WHEELER ,  Yiran ZHANG ,  Alireza SALMANZADEH

IPC: B01L3/00 ,  B01L7/00

Abstract: Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.

公开(公告)号：US20210053063A1

公开(公告)日：2021-02-25

申请号：US16998648

申请日：2020-08-20

Applicant: 10X Genomics, Inc.

Inventor： Mohammad Rahimi LENJI ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  B01L3/02

Abstract: The invention provides kits, devices, methods, and systems for forming droplets or particles and methods of their use. The devices may be used to form droplets of a size suitable for utilization as microscale chemical reactors, e.g., for genetic sequencing. In general, droplets are formed in a device by flowing a first liquid through a channel and into a droplet formation region including a second liquid, i.e., the continuous phase. The invention allows for more efficient recovery of droplets or processed droplets.