公开(公告)号：US20210388423A1

公开(公告)日：2021-12-16

申请号：US17346077

申请日：2021-06-11

Applicant: 10x Genomics, Inc.

Inventor： Felice Alessio BAVA ,  David M. PATTERSON ,  Meiliana TJANDRA ,  Yi LUO

IPC: C12Q1/6841 ,  C12Q1/6876

Abstract: Provided herein are methods of sequencing comprising click chemistry bioconjugation. In some embodiments, target polynucleotide sequences on the same or different molecules are contacted with and hybridize to probes comprising click functional groups. Probes (e.g., reading probes) hybridizing to adaptor sequences adjacent to different sequences of interest (e.g., barcodes to be sequenced) can be hybridized simultaneously in large pools. In some embodiments, the provided methods achieve multiplexing without requiring separate hybridization of probes (e.g., reading probes) for each sequencing-by-ligation cycle, thereby reducing total hybridization time which is typically a most time-consuming step in in situ technologies. In some aspects, the hybridized probes (e.g., reading probes) are clicked onto detectable probes to analyze a sequence of a target polynucleotide in a sequencing-by-ligation fashion.

公开(公告)号：US20240191297A1

公开(公告)日：2024-06-13

申请号：US18486918

申请日：2023-10-13

Applicant: 10x Genomics, Inc.

Inventor： Cheyenne CHRISTOPHERSON ,  Veronica Emelina GONZALEZ MUÑOZ ,  Joshua GU ,  Albert Dale KIM ,  Yi LUO ,  Meiliana TJANDRA

IPC: C12Q1/6881 ,  C12Q1/6806 ,  C12Q1/6841

CPC classification number: C12Q1/6881 ,  C12Q1/6806 ,  C12Q1/6841 ,  C12Q2600/166

Abstract: The present disclosure relates in some aspects to methods and compositions for quality control (e.g., assessing the level of fixation of a biological sample) and/or optimization of analyte detection in fixed samples. In some embodiments, the method can be used to process a fixed biological sample, the method comprising contacting the fixed biological sample with a nucleic acid stain and/or an actin stain; detecting an optical signal associated with the nucleic acid stain and/or an optical signal associated with the actin stain; comparing the detected optical signal(s) to one or more references to determine the quality of the biological sample. Single cell analysis, spatial array based analysis or in situ analysis can be performed using the processed biological sample.

公开(公告)号：US20230295556A1

公开(公告)日：2023-09-21

申请号：US18188164

申请日：2023-03-22

Applicant: 10x Genomics, Inc.

Inventor： Shalini GOHIL ,  Joshua DELANEY ,  Yi LUO ,  Adam LOWE ,  Jason C. BELL ,  Michael John Terry STUBBINGTON ,  Wyatt James MCDONNELL

IPC: C12M1/00 ,  B01L3/02 ,  B01L3/00

CPC classification number: C12M47/04 ,  B01L3/0241 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0652

Abstract: The present disclosure provides methods, compositions, and systems for selective enzymatic gelation of cells, e.g., immune cells, contained in partitions.