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公开(公告)号：US20210270838A1
公开(公告)日：2021-09-02
申请号：US17258576
申请日：2019-07-10
发明人： Gregory W. Sitton
IPC分类号： G01N33/58 , G01N33/542
摘要： Method of assaying for an analyte in a sample, and kits for performing the assay.
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公开(公告)号：US20230273119A1
公开(公告)日：2023-08-31
申请号：US18111056
申请日：2023-02-17
发明人： Bharat R. Acharya , Raj Rajagopal , Gregory W. Sitton , Benjamin G. Sonnek , Robert M. Biegler , Gilles J. Benoit , Timothy J. Lindquist , John A. Wheatley , Kristal L. Schutta , James A. Phipps
IPC分类号： G01N21/31
CPC分类号： G01N21/314 , G01N2021/317 , G01N2201/0453
摘要： An optical well is configured to receive a test sample for examining an optical characteristic of the sample at a first wavelength in a predetermined wavelength range. The optical well includes a wall having a bottom wall portion and a sidewall portion defining a chamber for receiving the test sample, and an optical film formed into a shape so that a portion of the sidewall portion includes a first portion of the optical film, and a portion of the bottom wall portion includes a second portion of the optical film. For a normally incident light, the microlayers in each of the first and second portions have an average optical reflectance of greater than about 80% in the predetermined wavelength range. The forming results in the plurality of microlayers of the integral formed optical film having a thinnest portion and a thickest portion having a thickness difference of at least 30%.
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公开(公告)号：US20210293795A1
公开(公告)日：2021-09-23
申请号：US17258568
申请日：2019-07-10
发明人： Gregory W. Sitton
IPC分类号： G01N33/542
摘要： Method of assaying for an analyte in a sample, and kits for performing the assay.
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公开(公告)号：US11168352B2
公开(公告)日：2021-11-09
申请号：US16087750
申请日：2017-03-24
发明人： Gregory W. Sitton , Neil Percy
IPC分类号： C12Q1/6806 , C12Q1/686 , C12N1/06
摘要： The present disclosure provides a nucleic acid amplification method. The method comprises forming an enrichment culture by contacting a sample with a nutrient medium having a formulation that does not include a phosphate buffer component; holding the enrichment culture for a period of time at a temperature that facilitates growth of a target microorganism; after holding the enrichment culture, forming an aqueous composition by mixing a first volume of the enrichment culture with a second volume of a lysis buffer; contacting the aqueous composition with an effective amount of a water-insoluble material that sequesters a substance that interferes with a polymerase-mediated nucleic acid amplification reaction; subjecting the aqueous composition to a thermal lysis process; and, after subjecting the aqueous composition to the thermal lysis process, subjecting a portion of the aqueous composition to a nucleic acid amplification process. A composition for a lysis buffer is also disclosed.
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公开(公告)号：US10604787B2
公开(公告)日：2020-03-31
申请号：US15535504
申请日：2015-12-21
发明人： Gregory W. Sitton , Neil Percy
IPC分类号： C12Q1/68 , C12N15/10 , C12Q1/6806 , C12Q1/6844
摘要： A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes effective amounts of ferric iron, an organic iron-chelating reagent, and a non-ionic surfactant. Optionally, the composition includes polyvinylpyrrolidone. The composition has a pH of about 8.45 to 8.85. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium. The first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20° C. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.
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公开(公告)号：US10281466B2
公开(公告)日：2019-05-07
申请号：US15514139
申请日：2015-10-05
发明人： Neil Percy , Gregory W. Sitton
IPC分类号： G01N33/558 , G01N33/569 , B01D15/08 , C12Q1/6888 , G01N1/40 , G01N30/14 , G01N33/543 , G01N30/02
摘要： A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target analyte-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target analyte-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target analyte-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target analyte are also provided.
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公开(公告)号：US09845498B2
公开(公告)日：2017-12-19
申请号：US14487144
申请日：2014-09-16
发明人： Neil Percy , Gregory W. Sitton
CPC分类号： C12Q1/686 , G01N21/03 , G01N21/76 , G01N21/763
摘要： A method of detecting an analyte is provided. The method includes providing a sample, a container 110 with a wall 115, and a catalyst for a luminescent reaction. The wall includes a colored portion 115b. The method further comprises forming a reaction in the container and detecting the presence or absence of light emitted from the reaction mixture in the container. Detecting light emitted from the container can comprise detecting light passing through the colored portion. The colored portion can be detected visually and the color can be associated with the identity of an analyte-specific reagent disposed in the container. Kits comprising the container and a catalyst for a luminescent reaction are also provided.
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公开(公告)号：US20220161254A1
公开(公告)日：2022-05-26
申请号：US17617073
申请日：2020-06-23
发明人： Gregory W. Sitton
摘要： Articles, systems, and methods for transferring liquids that may employ a robot.
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公开(公告)号：US20210277352A1
公开(公告)日：2021-09-09
申请号：US17252401
申请日：2019-06-13
IPC分类号： C12N1/20
摘要： Salts of Fe (II), Zn (II), or both used in methods for enriching food matrices containing antimicrobial compounds. A kit comprising salts of Fe (II), Zn (II) useful for such methods.
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