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公开(公告)号:US5844093A
公开(公告)日:1998-12-01
申请号:US553497
申请日:1995-11-17
申请人: A. Cathrine Kettleborough , Mary M. Bendig , Keith H. Ansell , Detlef Gussow , Jaume Adan , Francesc Mitjans , Elisabet Rosell , Francesc Blasco , Jaume Piulats
发明人: A. Cathrine Kettleborough , Mary M. Bendig , Keith H. Ansell , Detlef Gussow , Jaume Adan , Francesc Mitjans , Elisabet Rosell , Francesc Blasco , Jaume Piulats
IPC分类号: G01N33/53 , A61K38/00 , A61K39/395 , A61P35/00 , C07K16/00 , C07K16/28 , C12N15/09 , C12N15/13 , C12P21/02 , C12P21/08 , C12R1/19 , G01N33/563 , C07H21/04 , C12N15/63
CPC分类号: C07K16/00 , C07K16/2863 , A61K38/00 , C07K2319/00
摘要: This invention relates to new anti-EGFR antibodies and single-chain Fvs (scFvs) thereof which can be obtained from phage-antibody libraries constructed from cells of an immunized mammalian, preferably a mouse. Two of the single-chain Fvs isolated from the phage-antibody libraries were engineered to create partially humanized whole antibody molecules. These chimeric anti-EGFR antibodies contain constant regions of human immunoglobulins, and can be used as well as the single-chain Fvs as agents for the diagnosis and therapy of human tumors.
摘要翻译: PCT No.PCT / EP95 / 00978 371日期:1995年11月17日 102(e)日期1995年11月17日PCT 1995年3月16日PCT公布。 公开号WO95 / 25167 日期1995年9月21日本发明涉及可从免疫的哺乳动物,优选小鼠的细胞构建的噬菌体抗体文库获得的新的抗EGFR抗体及其单链Fvs(scFv)。 将从噬菌体 - 抗体文库分离的两条单链Fvs进行工程改造以产生部分人源化的全抗体分子。 这些嵌合抗EGFR抗体含有人免疫球蛋白的恒定区域,并且可以用作单链Fvs作为人肿瘤诊断和治疗的试剂。
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公开(公告)号:US20090156457A1
公开(公告)日:2009-06-18
申请号:US10597140
申请日:2005-01-17
申请人: Keith H. Ansell
发明人: Keith H. Ansell
IPC分类号: A61K38/00 , G01N33/53 , C07K2/00 , C12N9/16 , C07H21/04 , C12N15/63 , C12N5/00 , C12P21/06 , C12Q1/00
CPC分类号: G01N33/68 , C07K2319/20 , C12N9/6424 , C12Q1/37 , G01N33/5008
摘要: This invention relates to methods of screening for rhomboid modulating compounds using a substrate polypeptide has a core domain comprising a rhomboid cleavable TMD sequence linked to an upstream tag sequence. The core domain sequence is not susceptible to cleavage by non-rhomboid proteases so products of rhomboid dependent proteolysis products may be detected by determining the presence of the tag sequence. Rhomboid modulating compounds identified by the present methods may be useful in a range of therapeutic applications.
摘要翻译: 本发明涉及使用底物多肽筛选菱形调节化合物的方法,其具有包含与上游标签序列连接的菱形可切割TMD序列的核心结构域。 核心结构域序列不易被非菱形蛋白酶切割,因此可以通过确定标签序列的存在来检测菱形依赖性蛋白水解产物的产物。 通过本方法鉴定的菱形调节化合物可用于一系列治疗应用。
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