公开(公告)号：US10213967B2

公开(公告)日：2019-02-26

申请号：US14302243

申请日：2014-06-11

Applicant: ACADEMIA SINICA

Inventor： Keng-Hui Lin ,  Yi-Hsuan Lee

IPC: B29C67/20 ,  B29K77/00 ,  B29K105/00 ,  B29K105/04 ,  B29K105/24

Abstract: A fabricating device of a three-dimensional (3-D) scaffold comprises a bubble generator, a bubble mixing channel, a coagulating solution channel and a bubble collector. The bubble generator includes a gas channel and a gel solution channel crossing the gas channel. The bubble mixing channel is connected with a first outlet of the bubble generator. The coagulating solution channel is connected with the bubble mixing channel. The bubble collector is connected with a second outlet of the bubble mixing channel.

公开(公告)号：US11492581B2

公开(公告)日：2022-11-08

申请号：US16838901

申请日：2020-04-02

Applicant: Academia Sinica

Inventor： Keng-Hui Lin ,  Cheng-Kuang Huang

IPC: C12M1/32 ,  C12M1/00 ,  B01L3/00 ,  C12N5/00

Abstract: A microwell device and a method of manufacturing the same are provided. The microwell device includes a substrate and a plurality of microwells formed on the substrate. In addition, each of the microwells includes a cavity being recessed on the substrate and an opening, and the diameter of the opening is smaller than the largest inner diameter of the cavity. Furthermore, the microwells are curved.

公开(公告)号：US20130309770A1

公开(公告)日：2013-11-21

申请号：US13944776

申请日：2013-07-17

Applicant: Academia Sinica

Inventor： Keng-Hui Lin ,  Narayan Mishra ,  Yen-Liang Liu ,  Chen-Chie Wang

IPC: C12N5/077

CPC classification number: C12N5/0655 ,  B29C44/00 ,  C12M25/14 ,  C12N5/0068 ,  C12N2533/74

Abstract: Methods and apparatuses for using microfluidics to generate bubbles and using the generated bubbles to construct scaffolds and cell-holding structures for culturing biological samples or analytes. In one implementation, a scaffold for growing cells is provided to include a matrix of interconnected cavities formed from mixing a gas and a liquid containing a cross linkable material to produce a matrix of gas bubbles of substantially the same size and cross linking the cross linkable material to form a structure in which cells are grown. In another implementation, a scaffold apparatus for growing cells includes a ball of a cross linked material forming an exterior shell that encloses to form a hollow interior inside the ball and biological samples embedded in the external shell.

Abstract translation: 使用微流体产生气泡并使用生成的气泡构建用于培养生物样品或分析物的支架和细胞保持结构的方法和装置。 在一个实施方案中，提供了用于生长细胞的支架，以包括通过混合气体和含有可交联材料的液体形成的互连腔的基质，以产生基本上相同尺寸的气泡基质并交联可交联材料 以形成细胞生长的结构。 在另一实施方案中，用于生长细胞的支架装置包括形成外壳的交联材料球，所述外壳包围以形成球内的中空内部并嵌入外壳中的生物样品。

公开(公告)号：US09957481B2

公开(公告)日：2018-05-01

申请号：US13944776

申请日：2013-07-17

Applicant: Academia Sinica

Inventor： Keng-Hui Lin ,  Narayan Mishra ,  Yen-Liang Liu ,  Chen-Chie Wang

IPC: C12N5/077 ,  B29C44/00 ,  C12N5/00 ,  C12M1/12

CPC classification number: C12N5/0655 ,  B29C44/00 ,  C12M25/14 ,  C12N5/0068 ,  C12N2533/74

Abstract: Methods and apparatuses for using microfluidics to generate bubbles and using the generated bubbles to construct scaffolds and cell-holding structures for culturing biological samples or analytes. In one implementation, a scaffold for growing cells is provided to include a matrix of interconnected cavities formed from mixing a gas and a liquid containing a cross linkable material to produce a matrix of gas bubbles of substantially the same size and cross linking the cross linkable material to form a structure in which cells are grown. In another implementation, a scaffold apparatus for growing cells includes a ball of a cross linked material forming an exterior shell that encloses to form a hollow interior inside the ball and biological samples embedded in the external shell.