公开(公告)号：US20140131204A1

公开(公告)日：2014-05-15

申请号：US13675735

申请日：2012-11-13

Applicant: ACADEMIA SINICA

Inventor： Chia-Fu Chou ,  Kuo-Tang Liao

IPC: G01N27/447

CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/06 ,  G01N15/0612 ,  G01N27/44713 ,  G01N2015/0065

Abstract: Methods, structures, devices and systems are disclosed for rapid enrichment and mass transport of biomolecules (e.g., such as proteins) or other small molecules and particles using electrodeless dielectrophoresis (eDEP). In one aspect, a device to aggregate molecules includes a substrate that is electrically insulating, an electrically insulative material formed on the substrate and structured to form a channel to carry an electrically conducting fluid containing particles, a constriction structure formed of the electrically insulative material and located in the channel to narrow a channel dimension and forming an opening with a size in the nanometer range, and a circuit coupled to the substrate to apply an ac electric field and a dc bias electric field along the channel, in which the constriction structure is structured to magnify the applied ac electric field to produce forces that operate collectively to aggregate the particles.

Abstract translation: 公开了用于使用无电极介电电泳（eDEP）快速富集和质量传递生物分子（例如蛋白质）或其它小分子和颗粒的方法，结构，装置和系统。 一方面，用于聚集分子的装置包括电绝缘的基板，形成在基板上并构造成形成用于承载含有颗粒的导电流体的通道的电绝缘材料，由电绝缘材料形成的收缩结构， 位于所述通道中以使通道尺寸变窄并形成尺寸在纳米范围内的开口，以及耦合到所述衬底的电路，沿着所述通道施加交流电场和直流偏置电场，其中所述收缩结构为 结构化以放大施加的交流电场以产生共同操作以聚集颗粒的力。

公开(公告)号：US09387488B2

公开(公告)日：2016-07-12

申请号：US13675735

申请日：2012-11-13

Applicant: Academia Sinica

Inventor： Chia-Fu Chou ,  Kuo-Tang Liao

IPC: G01N27/447 ,  B03C5/00 ,  B03C5/02 ,  B01L3/00 ,  G01N15/00 ,  G01N15/06

CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/06 ,  G01N15/0612 ,  G01N27/44713 ,  G01N2015/0065

Abstract: Methods, structures, devices and systems are disclosed for rapid enrichment and mass transport of biomolecules (e.g., such as proteins) or other small molecules and particles using electrodeless dielectrophoresis (eDEP). In one aspect, a device to aggregate molecules includes a substrate that is electrically insulating, an electrically insulative material formed on the substrate and structured to form a channel to carry an electrically conducting fluid containing particles, a constriction structure formed of the electrically insulative material and located in the channel to narrow a channel dimension and forming an opening with a size in the nanometer range, and a circuit coupled to the substrate to apply an ac electric field and a dc bias electric field along the channel, in which the constriction structure is structured to magnify the applied ac electric field to produce forces that operate collectively to aggregate the particles.

Abstract translation: 公开了用于使用无电极介电电泳（eDEP）快速富集和质量传递生物分子（例如蛋白质）或其它小分子和颗粒的方法，结构，装置和系统。 一方面，用于聚集分子的装置包括电绝缘的基板，形成在基板上并构造成形成用于承载含有颗粒的导电流体的通道的电绝缘材料，由电绝缘材料形成的收缩结构， 位于所述通道中以使通道尺寸变窄并形成尺寸在纳米范围内的开口，以及耦合到所述衬底的电路，沿着所述通道施加交流电场和直流偏置电场，其中所述收缩结构为 结构化以放大施加的交流电场以产生共同操作以聚集颗粒的力。