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    NANOFLUIDIC DEVICES FOR SINGLE-MOLECULE ANALYSIS OF PROTEIN-DNA COMPLEX
    1.
    发明申请
    NANOFLUIDIC DEVICES FOR SINGLE-MOLECULE ANALYSIS OF PROTEIN-DNA COMPLEX 有权
    用于单分子分析蛋白质-DNA复合物的纳米设备

    公开(公告)号：US20140120522A1

    公开(公告)日：2014-05-01

    申请号：US14048539

    申请日：2013-10-08

    Applicant: Academia Sinica

    Inventor： Chia-Fu Chou ,  K.K. Sriram

    IPC: C12Q1/70

    CPC classification number: B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0822 ,  B01L2400/0415 ,  Y10T29/49117

    Abstract: A device for optical mapping of protein binding sites, in particular, transcription factor binding sites, on single DNA molecules, includes an insulating substrate having two parallel channels and at least one slit connecting the two channels, a coverslip on the substrate, at least two reservoirs on the substrate connecting the channels of the insulating substrate, and at least two electrodes in the reservoirs. When the reservoirs are filled with a buffer solution, the electrodes are in electrical contact in the buffer solution.

    Abstract translation: 用于在单个DNA分子上的蛋白质结合位点，特别是转录因子结合位点的光学映射的装置包括具有两个平行通道的绝缘基底和连接两个通道的至少一个狭缝，在基底上的盖玻片，至少两个 连接绝缘基板的通道的基板上的储存器和储存器中的至少两个电极。 当储存器填充缓冲溶液时，电极在缓冲溶液中电接触。

    MOLECULAR ENTRAPMENT AND ENRICHMENT
    2.
    发明申请
    MOLECULAR ENTRAPMENT AND ENRICHMENT 有权
    分子生物学和分子生物学

    公开(公告)号：US20140131204A1

    公开(公告)日：2014-05-15

    申请号：US13675735

    申请日：2012-11-13

    Applicant: ACADEMIA SINICA

    Inventor： Chia-Fu Chou ,  Kuo-Tang Liao

    IPC: G01N27/447

    CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/06 ,  G01N15/0612 ,  G01N27/44713 ,  G01N2015/0065

    Abstract: Methods, structures, devices and systems are disclosed for rapid enrichment and mass transport of biomolecules (e.g., such as proteins) or other small molecules and particles using electrodeless dielectrophoresis (eDEP). In one aspect, a device to aggregate molecules includes a substrate that is electrically insulating, an electrically insulative material formed on the substrate and structured to form a channel to carry an electrically conducting fluid containing particles, a constriction structure formed of the electrically insulative material and located in the channel to narrow a channel dimension and forming an opening with a size in the nanometer range, and a circuit coupled to the substrate to apply an ac electric field and a dc bias electric field along the channel, in which the constriction structure is structured to magnify the applied ac electric field to produce forces that operate collectively to aggregate the particles.

    Abstract translation: 公开了用于使用无电极介电电泳（eDEP）快速富集和质量传递生物分子（例如蛋白质）或其它小分子和颗粒的方法，结构，装置和系统。 一方面，用于聚集分子的装置包括电绝缘的基板，形成在基板上并构造成形成用于承载含有颗粒的导电流体的通道的电绝缘材料，由电绝缘材料形成的收缩结构， 位于所述通道中以使通道尺寸变窄并形成尺寸在纳米范围内的开口，以及耦合到所述衬底的电路，沿着所述通道施加交流电场和直流偏置电场，其中所述收缩结构为 结构化以放大施加的交流电场以产生共同操作以聚集颗粒的力。

    Nanofluidic devices for single-molecule analysis of protein-DNA complex
    7.
    发明授权
    Nanofluidic devices for single-molecule analysis of protein-DNA complex 有权
    用于单分子分析蛋白质-DNA复合物的纳流体装置

    公开(公告)号：US09555412B2

    公开(公告)日：2017-01-31

    申请号：US14048539

    申请日：2013-10-08

    Applicant: Academia Sinica

    Inventor： Chia-Fu Chou ,  K. K. Sriram

    IPC: B01L3/00

    CPC classification number: B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0822 ,  B01L2400/0415 ,  Y10T29/49117

    Abstract: A device for optical mapping of protein binding sites, in particular, transcription factor binding sites, on single DNA molecules, includes an insulating substrate having two parallel channels and at least one slit connecting the two channels, a coverslip on the substrate, at least two reservoirs on the substrate connecting the channels of the insulating substrate, and at least two electrodes in the reservoirs. When the reservoirs are filled with a buffer solution, the electrodes are in electrical contact in the buffer solution.

    Abstract translation: 用于在单个DNA分子上的蛋白质结合位点，特别是转录因子结合位点的光学映射的装置包括具有两个平行通道的绝缘基底和连接两个通道的至少一个狭缝，在基底上的盖玻片，至少两个 连接绝缘基板的通道的基板上的储存器和储存器中的至少两个电极。 当储存器填充缓冲溶液时，电极在缓冲溶液中电接触。

    Molecular entrapment and enrichment
    8.
    发明授权
    Molecular entrapment and enrichment 有权
    分子捕获和富集

    公开(公告)号：US09387488B2

    公开(公告)日：2016-07-12

    申请号：US13675735

    申请日：2012-11-13

    Applicant: Academia Sinica

    Inventor： Chia-Fu Chou ,  Kuo-Tang Liao

    IPC: G01N27/447 ,  B03C5/00 ,  B03C5/02 ,  B01L3/00 ,  G01N15/00 ,  G01N15/06

    CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/06 ,  G01N15/0612 ,  G01N27/44713 ,  G01N2015/0065

    Abstract: Methods, structures, devices and systems are disclosed for rapid enrichment and mass transport of biomolecules (e.g., such as proteins) or other small molecules and particles using electrodeless dielectrophoresis (eDEP). In one aspect, a device to aggregate molecules includes a substrate that is electrically insulating, an electrically insulative material formed on the substrate and structured to form a channel to carry an electrically conducting fluid containing particles, a constriction structure formed of the electrically insulative material and located in the channel to narrow a channel dimension and forming an opening with a size in the nanometer range, and a circuit coupled to the substrate to apply an ac electric field and a dc bias electric field along the channel, in which the constriction structure is structured to magnify the applied ac electric field to produce forces that operate collectively to aggregate the particles.

    Abstract translation: 公开了用于使用无电极介电电泳（eDEP）快速富集和质量传递生物分子（例如蛋白质）或其它小分子和颗粒的方法，结构，装置和系统。 一方面，用于聚集分子的装置包括电绝缘的基板，形成在基板上并构造成形成用于承载含有颗粒的导电流体的通道的电绝缘材料，由电绝缘材料形成的收缩结构， 位于所述通道中以使通道尺寸变窄并形成尺寸在纳米范围内的开口，以及耦合到所述衬底的电路，沿着所述通道施加交流电场和直流偏置电场，其中所述收缩结构为 结构化以放大施加的交流电场以产生共同操作以聚集颗粒的力。

    MICROFLUIDIC SYSTEMS AND DEVICES FOR MOLECULAR CAPTURE, MANIPULATION, AND ANALYSIS
    9.
    发明申请
    MICROFLUIDIC SYSTEMS AND DEVICES FOR MOLECULAR CAPTURE, MANIPULATION, AND ANALYSIS 有权
    用于分子捕获，操作和分析的微流体系统和装置

    公开(公告)号：US20140320849A1

    公开(公告)日：2014-10-30

    申请号：US13872013

    申请日：2013-04-26

    Applicant: MAX-PLANCK-INSTITUT FÜR EISENFORSCHUNG GMBH ,  ACADEMIA SINICA

    Inventor： Chia-Fu Chou ,  Lesser-Rojas Leonardo ,  Ming-Li Chu ,  Andreas Erbe

    IPC: G01N27/26 ,  G01N21/65

    CPC classification number: G01N21/65 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/48721

    Abstract: Methods, systems, and devices are disclosed for molecular capture, manipulation, and analysis. In one aspect, a device to aggregate and characterize particles in a fluid includes an electrically insulative substrate including a channel to carry an electrically conducting fluid containing particles, electrodes located in the channel forming a nanoscale opening and including an insulating layer over their surface at the opening, a first circuit to apply a non-uniform ac electric field and a dc bias signal across the electrodes, in which the applied non-uniform ac electric field produces a positive dielectrophoretic force to aggregate the particles in a trapping region including the opening and adjacent region, a second circuit to detect changes in a dc current caused by at least some of the particles in the trapping region, and an optical device that directs a coherent light beam on the opening to determine Raman spectra of the particles in the trapping region.

    Abstract translation: 公开了用于分子捕获，操纵和分析的方法，系统和装置。 在一个方面，用于聚集和表征流体中的颗粒的装置包括电绝缘基板，其包括用于承载含有颗粒的导电流体的通道，位于通道中的电极形成纳米级开口，并且在其表面上包括绝缘层 打开，跨越电极施加不均匀交流电场和直流偏置信号的第一电路，其中所施加的不均匀交流电场产生积极的介电电泳力，以将颗粒聚集在包括开口的捕获区域中， 相邻区域，用于检测由捕获区域中的至少一些颗粒引起的直流电流的变化的第二电路，以及将相干光束引导到开口上以确定捕获区域中的颗粒的拉曼光谱的光学装置 。

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