公开(公告)号：US20240351029A1

公开(公告)日：2024-10-24

申请号：US18619661

申请日：2024-03-28

申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： Aleksei Aksimentiev

IPC分类号： B01L3/00 ,  C12Q1/6869

CPC分类号： B01L3/502715 ,  C12Q1/6869 ,  B01L2200/0652 ,  B01L2300/0663 ,  B01L2300/0896 ,  B01L2400/0415

摘要： Aspects of the subject disclosure may include, for example, an apparatus comprising: a membrane, wherein the membrane has a first side and a second side, wherein the membrane has a first pore disposed therein, wherein the first pore extends through the membrane from the first side of the membrane to the second side of the membrane, wherein the membrane has a second pore disposed therein, and wherein the second pore extends through the membrane from the first side of the membrane to the second side of the membrane; a first channel disposed on the first side of the membrane, wherein the first channel is along a first longitudinal axis; a second channel disposed on the first side of the membrane, wherein the second channel is along a second longitudinal axis, and wherein the first channel and the second channel are disposed side by side adjacent to each other; a third channel disposed on the second side of the membrane, wherein the third channel is along a third longitudinal axis, wherein the third channel is in first fluid communication with the first channel via the first pore, and wherein the third channel is in second fluid communication with the second channel via the second pore; and one or more sensors disposed at one or more locations to facilitate sequencing of a molecule that extends from the first channel, through the first pore across at least a portion of the third channel, and through the second pore into the second channel. Additional embodiments are disclosed.

公开(公告)号：US12103004B2

公开(公告)日：2024-10-01

申请号：US16980358

申请日：2019-03-12

申请人： SILICON VALLEY SCIENTIFIC, INC.

发明人： Stevan Bogdan Jovanovich

IPC分类号： B01L3/00 ,  C12N15/10 ,  G01N1/28 ,  G01N35/00 ,  G01N35/08 ,  G16B5/00

CPC分类号： B01L3/502784 ,  B01L3/502761 ,  B01L3/50857 ,  C12N15/1013 ,  G01N1/286 ,  G01N35/0099 ,  G01N35/08 ,  G16B5/00 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/18

摘要： Provided herein is a system comprising: a) spatial sampler system configured to collect and transmit one or a plurality of cells or nuclei from a tissue specimen, the system comprising: i) a lower carrier having an array of conduits passing therethrough, each conduit comprising an opening on a first side and communicating with an opening on a second side, wherein the opening on the second side either (1) terminates in a capillary or (2) opens onto a well of a multiwell plate; ii) positioned, above the lower carrier, a perforated specimen holder configured to support a frozen tissue specimen, wherein the specimen holder comprises a plurality of perforations having a size sufficient to permit the passage of single cells or nuclei; and iii) positioned, above the specimen holder, a multifunctional head comprising an upper array of upper conduits, optionally, each aligned with a conduit opening of the lower carrier.

公开(公告)号：US20240316556A1

公开(公告)日：2024-09-26

申请号：US18559286

申请日：2022-05-05

申请人： BioSkryb Genomics, Inc.

发明人： Jay A.A. West

IPC分类号： B01L3/00 ,  C12Q1/6844

CPC分类号： B01L3/502784 ,  B01L3/5085 ,  C12Q1/6844 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/14 ,  B01L2400/0487 ,  B01L2400/0688

摘要： Provided herein are compositions and methods for high-throughput Primary Template-Directed Amplification (PTA) nucleic acid amplification and sequencing methods, and their applications for mutational analysis in research, diagnostics, and treatment. Further provided herein are methods for parallel analysis of DNA, RNA, and/or proteins from single cells.

公开(公告)号：US20240165624A1

公开(公告)日：2024-05-23

申请号：US18359237

申请日：2023-07-26

申请人： PLENO, INC.

发明人： Pieter VAN ROOYEN ,  Lorenzo BERTI ,  Jeffrey BRODIN ,  Michael POLLACK

IPC分类号： B01L3/00 ,  G01N27/414

CPC分类号： B01L3/502792 ,  G01N27/4146 ,  G01N27/4148 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2400/0427

摘要： A droplet manipulation device comprising, which may, for example, include (a) a first substrate having a first layer comprising a first array of electrowetting electrodes, and a second layer atop a region of the first layer comprising a second array of electrowetting electrodes; and (b) a second substrate separated from the first substrate forming a droplet operations gap between the first and second substrates.

公开(公告)号：US20240151682A1

公开(公告)日：2024-05-09

申请号：US18550881

申请日：2022-03-15

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Quan QING

IPC分类号： G01N27/327 ,  B01L3/00

CPC分类号： G01N27/3278 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0896 ,  B01L2400/0415

摘要： Disclosed are systems and methods for delivering and/or linking molecules, such as DNA, between tunable metal nanogaps and measuring electrical and/or optical properties. In one example, disclosed are high density multiplexed chips with a plurality of groups, each group including a plurality of nanodevices, the chips capable of coupling to one or more multiwell structures for providing samples to each individual group. In this way, the chips can be used for high-throughput analysis of molecules such as DNA.

公开(公告)号：US11944966B2

公开(公告)日：2024-04-02

申请号：US17513516

申请日：2021-10-28

申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： Aleksei Aksimentiev

IPC分类号： B01L3/00 ,  C12Q1/6869

CPC分类号： B01L3/502715 ,  C12Q1/6869 ,  B01L2200/0652 ,  B01L2300/0663 ,  B01L2300/0896 ,  B01L2400/0415

摘要： Aspects of the subject disclosure may include, for example, an apparatus comprising: a membrane, wherein the membrane has a first side and a second side, wherein the membrane has a first pore disposed therein, wherein the first pore extends through the membrane from the first side of the membrane to the second side of the membrane, wherein the membrane has a second pore disposed therein, and wherein the second pore extends through the membrane from the first side of the membrane to the second side of the membrane; a first channel disposed on the first side of the membrane, wherein the first channel is along a first longitudinal axis; a second channel disposed on the first side of the membrane, wherein the second channel is along a second longitudinal axis, and wherein the first channel and the second channel are disposed side by side adjacent to each other; a third channel disposed on the second side of the membrane, wherein the third channel is along a third longitudinal axis, wherein the third channel is in first fluid communication with the first channel via the first pore, and wherein the third channel is in second fluid communication with the second channel via the second pore; and one or more sensors disposed at one or more locations to facilitate sequencing of a molecule that extends from the first channel, through the first pore across at least a portion of the third channel, and through the second pore into the second channel. Additional embodiments are disclosed.

公开(公告)号：US11919003B2

公开(公告)日：2024-03-05

申请号：US17577472

申请日：2022-01-18

申请人： THE UNIVERSITY OF HONG KONG

发明人： Liqiu Wang ,  Wei Li ,  Xin Tang

IPC分类号： B01L3/00 ,  C12M3/06 ,  G01N35/00 ,  G03F7/00 ,  G03F7/038 ,  G03F7/20 ,  G03F7/32 ,  G06N3/08 ,  G06N20/00 ,  H01F1/053 ,  H01F7/02

CPC分类号： B01L3/502784 ,  B01L3/50273 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2300/168 ,  B01L2400/022 ,  B01L2400/0415

摘要： Disclosed is a device for moving a liquid in a substantially loss-free operation, the device made of at least a photothermal film; a pyroelectric crystal over the photothermal film; and a superomniphobic surface over the pyroelectric crystal, wherein the device is configured to move the liquid in the substantially loss-free operation with a beam of light.

公开(公告)号：US11904318B2

公开(公告)日：2024-02-20

申请号：US17450931

申请日：2021-10-14

申请人： Iowa State University Research Foundation, Inc.

发明人： Robbyn K. Anand ,  Sungu Kim ,  Baskar Ganapathysubramanian

IPC分类号： G01N33/68 ,  G01N21/33 ,  G01N21/64 ,  G01N33/52 ,  C07C309/65 ,  C07C309/73 ,  A01N1/02 ,  G01N1/40 ,  B01L3/00

CPC分类号： B01L3/502792 ,  B01L3/50273 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2400/0421

摘要： Microfluidic devices and methods that utilize ion concentration polarization within water-in-oil nanoliter scale droplets for concentration enrichment, separation, and substitution of charges species are disclosed. Such devices and methods can be used for separation of multiple species by mobility of each species and for the alteration and manipulation of the droplet composition by ion exchange.

公开(公告)号：US11872561B2

公开(公告)日：2024-01-16

申请号：US17200038

申请日：2021-03-12

申请人： Robert Bosch GmbH

发明人： Karim Gadelrab ,  Mordechai Kornbluth ,  Jake Christensen ,  Christopher Johnson ,  Boris Kozinsky

IPC分类号： G01N33/68 ,  G01N21/33 ,  G01N21/64 ,  G01N33/52 ,  C07C309/65 ,  C07C309/73 ,  A01N1/02 ,  G01N1/40 ,  B01L3/00 ,  C12M1/42 ,  C12N13/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  G01N33/487

CPC分类号： B01L3/502761 ,  C12M35/02 ,  C12N13/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  B01L2200/0663 ,  B01L2300/0896 ,  B01L2400/0415 ,  G01N33/48721 ,  C12Q1/6806 ,  C12Q2523/303 ,  C12Q2563/116 ,  C12Q2565/629 ,  C12Q2565/631

摘要： A system for stretching a polynucleotide structure includes a first electrode configured to generate an electrostatic force perpendicular to a surface of the first electrode and to apply the electrostatic force to the polynucleotide structure to pin an end region of the polynucleotide structure near the surface of the first electrode, and a second electrode configured to generate an electric force along an axial direction of the polynucleotide structure to stretch the polynucleotide structure along the axial direction of the polynucleotide structure into a fully extended form.

公开(公告)号：US20230390763A1

公开(公告)日：2023-12-07

申请号：US18234004

申请日：2023-08-15

申请人： International Business Machines Corporation

发明人： Lawrence A. Clevenger ,  Kangguo Cheng ,  Donald Canaperi ,  Shawn Peter Fetterolf

IPC分类号： B01L3/00 ,  B82B1/00 ,  B82B3/00

CPC分类号： B01L3/502707 ,  B82B1/001 ,  B82B3/0019 ,  B01L3/502715 ,  B01L2300/12 ,  B01L2200/12 ,  B01L2300/0896 ,  B01L2300/0645

摘要： 3D nanochannel interleaved devices for molecular manipulation are provided. In one aspect, a method of forming a device includes: forming a pattern on a substrate of alternating mandrels and spacers alongside the mandrels; selectively removing the mandrels from a front portion of the pattern forming gaps between the spacers; selectively removing the spacers from a back portion of the pattern forming gaps between the mandrels; filling i) the gaps between the spacers with a conductor to form first electrodes and ii) the gaps between the mandrels with the conductor to form second electrodes; and etching the mandrels and the spacers in a central portion of the pattern to form a channel (e.g., a nanochannel) between the first electrodes and the second electrodes, wherein the first electrodes and the second electrodes are offset from one another across the channel, i.e., interleaved. A device formed by the method is also provided.