公开(公告)号：US20140093432A1

公开(公告)日：2014-04-03

申请号：US14095120

申请日：2013-12-03

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Hong Tan ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Robert F. Zuk

IPC: G01N21/45

CPC classification number: G01N21/7703 ,  G01J3/45 ,  G01N21/45 ,  G01N21/648 ,  G01N21/8507 ,  G01N2021/7779 ,  G01N2201/062 ,  G01N2201/08 ,  G02B6/262 ,  G02B6/30

Abstract: The present invention is directed to an assembly for use in detecting an analyte in a sample based on thin-film spectral interference. The assembly comprises a waveguide, a monolithic substrate optically coupled to the waveguide, and a thin-film layer directly bonded to the sensing side of the monolithic substrate. The refractive index of the monolithic substrate is higher than the refractive index of the transparent material of the thin-film layer. A spectral interference between the light reflected into the waveguide from a first reflecting surface and a second reflecting surface varies as analyte molecules in a sample bind to the analyte binding molecules coated on the thin-film layer.

Abstract translation: 本发明涉及一种用于基于薄膜光谱干涉检测样品中的分析物的组件。 组件包括波导，光耦合到波导的单片基板，以及直接结合到整体式基板的感测侧的薄膜层。 单片基板的折射率高于薄膜层的透明材料的折射率。 当样品中的分析物分子结合到涂覆在薄膜层上的分析物结合分子时，从第一反射表面和第二反射表面反射到波导中的光之间的光谱干涉变化。

公开(公告)号：US08492139B2

公开(公告)日：2013-07-23

申请号：US13659576

申请日：2012-10-24

Applicant: Access Medical Systems, Ltd.

Inventor： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

IPC: C12M1/34 ,  G01N33/544

CPC classification number: G01N33/54366 ,  C08B37/006 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2201/06113

Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≦5 mm) and a high molecular weight polymer (≧1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.

Abstract translation: 本发明涉及一种用于在荧光测定中测量荧光信号的检测系统。 该系统包括具有与荧光标签结合的小感测表面的探针，以及安装在基底的感测表面的近侧的光源和检测器。 本发明还涉及使用具有小表面积（@ 5mm）和具有多个结合分子和多个荧光标记的高分子量聚合物（> = 1 MD）的探针尖端来检测液体样品中的分析物的方法。 通过横向流动反应溶液并在反应容器中上下移动探针尖端来加速结合反应。 本发明进一步涉及包含具有多个结合分子和多个荧光标记的交联FICOLL®分子的荧光标记组合物。

公开(公告)号：US10379116B2

公开(公告)日：2019-08-13

申请号：US15256319

申请日：2016-09-02

Applicant: Access Medical Systems, LTD.

Inventor： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

IPC: G01N33/543 ,  G01N21/64 ,  C08B37/00

Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≤5 mm) and a high molecular weight polymer (≥1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.