公开(公告)号：US09051535B2

公开(公告)日：2015-06-09

申请号：US13851033

申请日：2013-03-26

Applicant: ADVANCED BIOCATALYTICS CORP.

Inventor： Michael G. Goldfeld ,  Andrew H. Michalow ,  Carl W. Podella ,  John W. Baldridge

IPC: C12N9/96 ,  C11D3/386 ,  C12P19/02 ,  C11D3/38 ,  C12P19/14 ,  C12P21/00 ,  C12N9/20 ,  C12P7/64 ,  C12N9/42

CPC classification number: C11D3/38627 ,  C11D3/38 ,  C11D3/38636 ,  C11D3/38645 ,  C12N9/20 ,  C12N9/2437 ,  C12N9/96 ,  C12P7/6418 ,  C12P19/02 ,  C12P19/14 ,  C12P21/00

Abstract: Disclosed herein are compositions containing enzymes, particularly acting at the interface between two immiscible phases where the rate of enzymatic activity is increased by addition of a blend of surfactant(s) and a mixture derived from yeast fermentation, that contain non-enzymatic exo-proteins released by yeast in response to a non-lethal stress. The enzymes include those that work at the interface between an aqueous solution and a water immiscible phase, liquid or solid, such as oil, fat, cellulose, lignin, etc. including, but not limited to the following or combinations thereof: lipases, polysaccharase, lignase, cellulase and the like, in which the substrate of an enzymatic reaction forms a phase, segregated from the aqueous solution in which the enzymes are typically operating. Disclosed herein are methods for improving a washing solution with the use of these compositions, where the enzyme-protein-surfactant solution can be used in such applications as: laundry, spot remover, pre-laundry, dishes, hard surface cleaning, wastewater treatment, cellulose breakdown as in ethanol production, lignin utilization, environmental remediation, industrial cleaning, and agricultural applications.

Abstract translation: 本文公开了含有酶的组合物，特别是在两个不混溶相之间的界面处作用，其中酶活性的速率通过加入表面活性剂的混合物和衍生自酵母发酵的混合物而增加，其含有非酶促外切蛋白 由酵母释放，以应对非致死胁迫。 酶包括在水溶液和水不混溶相（液体或固体）如油，脂肪，纤维素，木质素等之间的界面处起作用的酶，包括但不限于以下或其组合：脂肪酶，多糖酶 ，木质素酶，纤维素酶等，其中酶反应的底物形成与酶通常在其中操作的水溶液分离的相。 本文公开了使用这些组合物改进洗涤溶液的方法，其中酶 - 蛋白质 - 表面活性剂溶液可用于以下应用：洗衣，去斑剂，预洗衣物，盘子，硬表面清洁，废水处理， 纤维素分解如乙醇生产，木质素利用，环境修复，工业清洁和农业应用。