公开(公告)号：US20160051992A1

公开(公告)日：2016-02-25

申请号：US14928096

申请日：2015-10-30

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： PRASANNA THWAR ,  VAMSEE K. PAMULA ,  ARJUN SUDARSAN ,  RAMAKRISHNA SISTA

IPC: B03C5/00 ,  B03C5/02 ,  B03C1/01

CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

公开(公告)号：US20150238975A1

公开(公告)日：2015-08-27

申请号：US14697058

申请日：2015-04-27

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： VAMSEE K. PAMULA ,  ARJUN SUDARSAN ,  RAMAKRISHNA SISTA

IPC: B03C5/00

CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

公开(公告)号：US20140193807A1

公开(公告)日：2014-07-10

申请号：US14163442

申请日：2014-01-24

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： VAMSEE K. PAMULA ,  ARJUN SUDARSAN ,  RAMAKRISHNA SISTA

IPC: C12Q1/68 ,  G01N33/543

CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括提供包括磁响应珠的液滴。 液滴可以设置在具有足够强度的磁场的区域内，以将磁响应珠吸引到液滴的边缘或朝向液滴的边缘，或以其他方式区分或聚集液滴内的珠粒。 该方法还可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

公开(公告)号：US20160129437A1

公开(公告)日：2016-05-12

申请号：US14538506

申请日：2014-11-11

Applicant: GENMARK DIAGNOSTICS, INC. ,  ADVANCED LIQUID LOGIC, INC.

Inventor： JON FAIZ KAYYEM ,  SEAN FORD ,  VAMSEE K. PAMULA ,  VIJAY SRINIVASAN ,  SCOTT COREY ,  BRIAN MURPHY ,  DAVID WALTER WRIGHT ,  DOMINIC AIELLO

IPC: B01L3/00 ,  B01L7/00

CPC classification number: B01L3/502 ,  B01L3/502792 ,  B01L3/52 ,  B01L7/525 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/04 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.

Abstract translation: 在一个实施例中，多重流体处理盒包括样品阱，可变形流体室，与其中设置的混合器的混合井，包括裂解混合器的裂解室，用于微滴操作的电润湿栅格，以及配置成检测分析物的电传感器阵列 出于兴趣。 用于处理盒的装置被配置为接收盒并且选择性地将热能，磁力和电连接施加到盒上的一个或多个离散位置，并且还被配置成以指定的顺序压缩可变形室 。

公开(公告)号：US20150008128A1

公开(公告)日：2015-01-08

申请号：US14491231

申请日：2014-09-19

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： VAMSEE K. PAMULA ,  ARJUN SUDARSAN ,  RAMAKRISHNA SISTA

IPC: B03C5/00

CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.