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Patent Agency Ranking
公开(公告)号：US20160083690A1
公开(公告)日：2016-03-24
申请号：US14783545
申请日：2014-04-10
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： William Birch , Shaul Reuveny , Steve Oh , Jian Li
CPC classification number: C12N5/0075 , A61L27/18 , A61L27/34 , A61L27/3834 , C12N5/0654 , C12N2506/1392 , C12N2531/00 , C12N2533/30 , C12N2533/32 , C12N2533/40 , C12N2533/52 , C08L67/04 , C08L89/00 , C08L5/00
Abstract: A method for manufacturing polycaprolactone microcarriers is disclosed together with uses of the microcarriers.
Abstract translation: 公开了用于制造聚己内酯微载体的方法以及微载体的用途。
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公开(公告)号：US20210024883A1
公开(公告)日：2021-01-28
申请号：US16647137
申请日：2018-09-14
Applicant: Agency for Science, Technology and Research
Inventor： Jian Li , William Richard Nicholas Birch , Jessica Pei Wen Toh
Abstract: The present disclosure relates to emulsions, methods of preparation thereof, and uses of said emulsions to fabricate porous polymeric microspheres as microcarriers for cell culture. In particular, the present disclosure relates to an emulsion with enhanced stability, characterized in that the emulsion comprises a) a water phase, the water phase is an aqueous solution comprising a salt; and b) an oil phase, the oil phase comprising a polymer; wherein the oil phase is immiscible with the water phase, and wherein the density differential of the water phase and oil phase is less than about 0.02 g/cm3. In a preferred embodiment, the polymer is polycaprolactone (PCL).
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公开(公告)号：US20210380936A1
公开(公告)日：2021-12-09
申请号：US17288140
申请日：2019-10-23
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Youshan Melissa Lin , Steve Kah Weng Oh , Shaul Reuveny , William Richard Nicholas Birch , Jian Li , Asha Shekaran , Tin Lun Alan Lam
Abstract: The present invention refers to a method of manufacturing an implantable construct comprising chondrogenically differentiated cells and one or more polycaprolactone (PCL) microcarriers, an implantable construct produced using said method, and uses of the implantable construct. The present invention also refers to a method of manufacturing an implantable construct comprising mesenchymal stromal cells and one or more polycaprolactone (PCL) microcarriers, an implantable construct produced using said method, and uses of the implantable construct. The present invention further refers to a method of treating a disease or disorder associated with cartilage and/or bone defect, the method comprises administering one or more cell-free polycaprolactone (PCL) microcarriers in a patient suffering from the disease or disorder.
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公开(公告)号：US09458431B2
公开(公告)日：2016-10-04
申请号：US13968214
申请日：2013-10-29
Applicant: Agency for Science, Technology and Research
Inventor： Steve Oh , Shaul Reuveny , Jian Li , William Richard Nicholas Birch
IPC: C12N5/00 , C12N5/02 , C12N5/074 , C12N5/0735 , C12M1/12
CPC classification number: C12N5/0696 , C12M25/16 , C12N5/0606 , C12N2531/00 , C12N2533/10 , C12N2533/32 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/70 , C12N2533/78 , C12N2533/80 , C12N2533/90
Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
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公开(公告)号：US20140315300A1
公开(公告)日：2014-10-23
申请号：US13968214
申请日：2013-10-29
Applicant: Agency for Science, Technology and Research
Inventor： Steve Oh , Shaul Reuveny , Jian Li , William Richard Nicholas Birch
IPC: C12N5/074 , C12N5/0735
CPC classification number: C12N5/0696 , C12M25/16 , C12N5/0606 , C12N2531/00 , C12N2533/10 , C12N2533/32 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/70 , C12N2533/78 , C12N2533/80 , C12N2533/90
Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
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