公开(公告)号：US20160143955A1

公开(公告)日：2016-05-26

申请号：US14903672

申请日：2014-07-10

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Shigeki Sugii ,  Wee Kiat Ong

IPC: A61K35/35 ,  C12N5/077

CPC classification number: A61K35/35 ,  A61K35/12 ,  A61K35/36 ,  C12N5/0653 ,  C12N5/0667 ,  C12N2527/00

Abstract: Disclosed is a method for recovering stromal vascular fraction from a sample. The method comprises providing a mechanical force capable of breaking the sample into a single cell suspension whilst maintaining intact stromal vascular fraction cells cellular structures, wherein the mechanical force is mincing and/or homogenization. Also disclosed are methods of using the stromal vascular fraction.

Abstract translation: 公开了一种从样品中回收基质血管部分的方法。 该方法包括提供能够将样品破碎成单细胞悬浮液同时保持完整的基质血管部分细胞细胞结构的机械力，其中机械力是切碎和/或均质化。 还公开了使用基质血管部分的方法。

公开(公告)号：US20190086389A1

公开(公告)日：2019-03-21

申请号：US16100152

申请日：2018-08-09

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Shigeki Sugii ,  Wee Kiat Ong ,  Weiping Han

IPC: G01N33/50 ,  G01N33/68 ,  C12N5/0775

Abstract: Disclosed is a method of identifying the origin of adipose-derived stem cells, comprising detecting at least one cell-surface marker selected from a group consisting of CD 10, CD141, CD 142 and CD200. Also disclosed are methods of determining adipogenic capability of a cell capable of adipogenesis by determining the presence of CD 10 or CD200.