公开(公告)号：US20160139134A1

公开(公告)日：2016-05-19

申请号：US15003235

申请日：2016-01-21

Applicant: AJINOMOTO CO., INC.

Inventor： Hiroshi MIYANO ,  Akihisa YAHASHI ,  Kazutaka SHIMBO ,  Masakazu NAKAZAWA ,  Kazuo HIRAYAMA

IPC: G01N33/58 ,  C07D207/46 ,  G01N33/68

CPC classification number: G01N33/58 ,  C07D207/46 ,  G01N33/6803 ,  G01N33/6806 ,  G01N33/6848 ,  G01N2458/15 ,  G01N2560/00 ,  Y10T436/13 ,  Y10T436/14 ,  Y10T436/145555 ,  Y10T436/24

Abstract: The present invention provides a method for the analysis of a compound with amino group (e.g., an amino acid or peptide) contained in a sample and convenient manner with a high sensitivity. The compound with amino group in a sample containing the compound with amino group is labeled with a specific carbamate compound such as p-trimethylammonium anilyl-N-hydroxysuccinimidyl carbamate iodide to enhance the selectivity and sensitivity. The present invention is preferably used in conjunction with mass spectrometry such as MS/MS method to facilitate quantitative analysis. The present invention further provides labeling reagents for mass spectrometry.

Abstract translation: 本发明提供了用于分析样品中所含的氨基（例如氨基酸或肽）的化合物的方法，并且以高灵敏度方便地进行分析。 含有氨基化合物的样品中具有氨基的化合物用特定的氨基甲酸酯化合物如对三甲基铵苯乙烯基-N-羟基琥珀酰亚胺基氨基甲酸酯碘化物进行标记以提高选择性和灵敏度。 本发明优选与诸如MS / MS方法的质谱结合使用以促进定量分析。 本发明还提供用于质谱的标记试剂。