公开(公告)号：US11384379B2

公开(公告)日：2022-07-12

申请号：US16826656

申请日：2020-03-23

Applicant: AJINOMOTO CO., INC.

Inventor： Daiki Yahagi ,  Erika Yoshida ,  Hiroaki Fukada ,  Mitsunori Tokura ,  Uno Tagami ,  Masayuki Sugiki

IPC: C12P21/00 ,  C12N1/14 ,  C12N9/42 ,  C12P19/12

Abstract: A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturing Talaromyces cellulolyticus in a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.

公开(公告)号：US09970031B2

公开(公告)日：2018-05-15

申请号：US15630115

申请日：2017-06-22

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Hara ,  Keita Fukui ,  Daiki Yahagi

IPC: C12P7/46 ,  C07K14/245 ,  C12N1/20 ,  C12N15/10

CPC classification number: C12P7/46 ,  C07K14/245 ,  C12N1/20 ,  C12N15/1031 ,  C12N15/1082 ,  C12P7/50

Abstract: A method for producing a dicarboxylic acid is provided. A dicarboxylic acid is produced by culturing a bacterium having a dicarboxylic acid-producing ability, which has been modified so that the expression of one or more of the yeeA gene, ynfM gene, yjjP gene, and yjjB gene is increased, in a medium, and collecting the dicarboxylic acid from the medium.

公开(公告)号：US20220002772A1

公开(公告)日：2022-01-06

申请号：US17480792

申请日：2021-09-21

Applicant: AJINOMOTO CO., INC.

Inventor： Daiki Yahagi

IPC: C12P19/14

Abstract: A method for producing allolactose is provided. Allolactose is produced by bringing cells of a microorganism having beta-galactosidase (BGL) into contact with lactose in the presence of a substance that inhibits hydrolysis of allolactose.

公开(公告)号：US20200263219A1

公开(公告)日：2020-08-20

申请号：US16826656

申请日：2020-03-23

Applicant: AJINOMOTO CO., INC.

Inventor： Daiki Yahagi ,  Erika Yoshida ,  Hiroaki Fukada ,  Mitsunori Tokura ,  Uno Tagami ,  Masayuki Sugiki

IPC: C12P21/00 ,  C12N1/14 ,  C12N9/42 ,  C12P19/12

Abstract: A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturing Talaromyces cellulolyticus in a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.

公开(公告)号：US20170298397A1

公开(公告)日：2017-10-19

申请号：US15630115

申请日：2017-06-22

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Hara ,  Keita Fukui ,  Daiki Yahagi

IPC: C12P7/46 ,  C12N15/10 ,  C12N1/20 ,  C07K14/245

CPC classification number: C12P7/46 ,  C07K14/245 ,  C12N1/20 ,  C12N15/1031 ,  C12N15/1082 ,  C12P7/50

Abstract: A method for producing a dicarboxylic acid is provided. A dicarboxylic acid is produced by culturing a bacterium having a dicarboxylic acid-producing ability, which has been modified so that the expression of one or more of the yeeA gene, ynfM gene, yjjP gene, and yjjB gene is increased, in a medium, and collecting the dicarboxylic acid from the medium.