公开(公告)号：US11746342B2

公开(公告)日：2023-09-05

申请号：US16840945

申请日：2020-04-06

Applicant: AJINOMOTO CO., INC.

Inventor： Hiroaki Fukada ,  Hiroka Koguchi ,  Mitsunori Tokura

IPC: C12N9/48 ,  C12P21/04 ,  C12N9/58 ,  C12P1/02 ,  C12P21/02 ,  C12N15/09 ,  C07K14/37

CPC classification number: C12N9/58 ,  C12N15/09 ,  C12P1/02 ,  C12P21/02 ,  C12Y304/21048 ,  C07K14/37 ,  C12N2330/50

Abstract: A method for producing a protein is provided. An objective protein is produced by culturing Talaromyces cellulolyticus having an objective protein-producing ability, which has been modified so that the activity of a YscB protein is reduced, in a culture medium.

公开(公告)号：US11384379B2

公开(公告)日：2022-07-12

申请号：US16826656

申请日：2020-03-23

Applicant: AJINOMOTO CO., INC.

Inventor： Daiki Yahagi ,  Erika Yoshida ,  Hiroaki Fukada ,  Mitsunori Tokura ,  Uno Tagami ,  Masayuki Sugiki

IPC: C12P21/00 ,  C12N1/14 ,  C12N9/42 ,  C12P19/12

Abstract: A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturing Talaromyces cellulolyticus in a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.

公开(公告)号：US20200263219A1

公开(公告)日：2020-08-20

申请号：US16826656

申请日：2020-03-23

Applicant: AJINOMOTO CO., INC.

Inventor： Daiki Yahagi ,  Erika Yoshida ,  Hiroaki Fukada ,  Mitsunori Tokura ,  Uno Tagami ,  Masayuki Sugiki

IPC: C12P21/00 ,  C12N1/14 ,  C12N9/42 ,  C12P19/12

Abstract: A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturing Talaromyces cellulolyticus in a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.

公开(公告)号：US20200255869A1

公开(公告)日：2020-08-13

申请号：US16840945

申请日：2020-04-06

Applicant: AJINOMOTO CO., INC.

Inventor： Hiroaki Fukada ,  Hiroka Koguchi ,  Mitsunori Tokura

IPC: C12P1/02 ,  C12P21/02

Abstract: A method for producing a protein is provided. An objective protein is produced by culturing Talaromyces cellulolyticus having an objective protein-producing ability, which has been modified so that the activity of a YscB protein is reduced, in a culture medium.

公开(公告)号：US20230212503A1

公开(公告)日：2023-07-06

申请号：US18063181

申请日：2022-12-08

Applicant: AJINOMOTO CO., INC.

Inventor： Nobuhisa Nitta ,  Hiroaki Fukada ,  Kazutaka Shimbo ,  Akiko Matsudaira ,  Toshiki Watanabe ,  Yoshihiro Usuda

IPC: C12N1/14

CPC classification number: C12N1/145 ,  C12N2501/734

Abstract: A method for producing a protein is provided. An objective protein is produced by culturing in a culture medium Talaromyces cellulolyticus having an objective protein-producing ability, which has been modified so that the activity of a Pep4 protein is reduced.