中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

20 records (took 0.017 seconds)

    Modified Enzyme and Method of Producing Imidazole Dipeptide Using Same
    1.
    发明公开
    Modified Enzyme and Method of Producing Imidazole Dipeptide Using Same 审中-公开

    公开(公告)号：US20240240167A1

    公开(公告)日：2024-07-18

    申请号：US18618330

    申请日：2024-03-27

    Applicant: AJINOMOTO CO., INC.

    Inventor： Mizuki Tamori ,  Uno Tagami ,  Hiroyuki Nozaki

    IPC: C12N9/48 ,  C12P21/02

    CPC classification number: C12N9/485 ,  C12P21/02

    Abstract: Described herein is an enzyme useful for producing a target peptide, capable of producing the target peptide without requiring ATP. More specifically, provided herein is a modified enzyme includes an amino acid sequence such as a modified amino acid sequence including a mutation of one or more of the following amino acid residues: E81, I127, I136, T139, F140, G142, W143, I147, I181, I201, Q219, T229, M244, A249, P255, E256, I260, S293, N294, Y295, and I299 in an amino acid sequence of SEQ ID NO: 1, and



    having the following property (a) or (b) enhanced relative to an enzyme of the amino acid sequence of SEQ ID NO: 1:
    (a) imidazole dipeptide production activity; or
    (b) thermal stability.

    Modified Phenylalanine Dehydrogenase
    3.
    发明申请
    Modified Phenylalanine Dehydrogenase 有权

    公开(公告)号：US20220235333A1

    公开(公告)日：2022-07-28

    申请号：US17714537

    申请日：2022-04-06

    Applicant: AJINOMOTO CO., INC.

    Inventor： Moemi Tatsumi ,  Kazutoshi Takahashi ,  Uno Tagami ,  Hiroki Yamaguchi

    IPC: C12N9/06 ,  C12N15/10 ,  C12N15/63 ,  C12N5/10

    Abstract: The present invention provides a unit and a method useful for more precise phenylalanine measurement. More specifically, the present invention provides a modified phenylalanine dehydrogenase that includes a mutation of at least one amino acid residue so as to improve the characteristics (for example, substrate specificity, solubility, and phenylalanine dehydrogenase activity) of a phenylalanine dehydrogenase related to measurement of phenylalanine, a method for analyzing phenylalanine by measuring phenylalanine contained in a test sample using the modified phenylalanine dehydrogenase, and others.

    MODIFIED ISOPRENE SYNTHASE
    5.
    发明申请
    MODIFIED ISOPRENE SYNTHASE 有权
    改良的ISOPRENE SYNTHASE

    公开(公告)号：US20160257944A1

    公开(公告)日：2016-09-08

    申请号：US15159140

    申请日：2016-05-19

    Applicant: AJINOMOTO CO., INC.

    Inventor： Kunio NAKATA ,  Yoshinori Tajima ,  Uno Tagami ,  Takashi Oku ,  Yasuhiro Kashima

    IPC: C12N9/88 ,  C08K3/22 ,  C08K3/04 ,  C12P5/00 ,  C08F36/08

    CPC classification number: C12N9/88 ,  C08F36/08 ,  C08K3/04 ,  C08K3/22 ,  C08K2003/2296 ,  C12N15/09 ,  C12P5/007 ,  C12P5/026 ,  C12Y402/03007 ,  C12Y402/03027 ,  C08L9/00

    Abstract: Modified isoprene synthases that have one or more mutations of a given amino acid residue(s) in any amino acid sequence of (a) the amino acid sequence of SEQ ID NO:4, (b) an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO:4, or (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:4, and has an isoprene synthetic activity are useful for preparing isoprene monomer in improved yields.

    Abstract translation: 在（a）SEQ ID NO：4的氨基酸序列的任何氨基酸序列中具有一个或多个给定氨基酸残基的一个或多个突变的修饰的异戊二烯合酶，​​（b）具有一个或几个氨基酸的氨基酸序列 在SEQ ID NO：4的氨基酸序列中的酸取代，缺失，插入或添加，或（c）与SEQ ID NO：4的氨基酸序列具有90％或更高同一性的氨基酸序列，并且具有异戊二烯 合成活性可用于以提高的产率制备异戊二烯单体。

    2-O-sulfation enzyme mutant and 3-O-sulfation enzyme mutant, and method for using same
    6.
    发明授权
    2-O-sulfation enzyme mutant and 3-O-sulfation enzyme mutant, and method for using same 有权

    公开(公告)号：US12139728B2

    公开(公告)日：2024-11-12

    申请号：US18056853

    申请日：2022-11-18

    Applicant: AJINOMOTO CO., INC.

    Inventor： Chihiro Tsuji ,  Tomoko Shimizu ,  Uno Tagami ,  Yasuhiro Mihara ,  Masayuki Sugiki ,  Shogo Nakano ,  Tomoharu Motoyama ,  Sohei Ito

    IPC: C12N9/10 ,  C08B37/00 ,  C12P19/26

    Abstract: The present invention provides a 2-OST mutant exhibiting a high activity. Specifically, the present invention provides a 2-O-sulfation enzyme mutant, having a substitution of a leucine residue at position 321 with a basic amino acid residue in any one amino acid sequence of: (a) the amino acid sequence of SEQ ID NO: 2; (b) an amino acid sequence comprising one or several amino acid substitutions, deletions, insertions, or additions in the amino acid sequence of SEQ ID NO: 2; (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO: 2; (d) the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; (e) an amino acid sequence comprising one or several amino acid substitutions, deletions, insertions, or additions in the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; (f) an amino acid sequence having 90% or more identity to the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; and having a 2-O-sulfate transfer activity.

    2-O-sulfation enzyme mutant and 3-O-sulfation enzyme mutant, and method for using same
    7.
    发明授权
    2-O-sulfation enzyme mutant and 3-O-sulfation enzyme mutant, and method for using same 有权

    公开(公告)号：US11851688B2

    公开(公告)日：2023-12-26

    申请号：US16795991

    申请日：2020-02-20

    Applicant: AJINOMOTO CO., INC.

    Inventor： Chihiro Tsuji ,  Tomoko Shimizu ,  Uno Tagami ,  Yasuhiro Mihara ,  Masayuki Sugiki ,  Shogo Nakano ,  Tomoharu Motoyama ,  Sohei Ito

    IPC: C12N9/10 ,  C08B37/00 ,  C12P19/26

    CPC classification number: C12N9/13 ,  C08B37/0075 ,  C12P19/26 ,  C12Y208/02

    Abstract: The present invention provides a 2-OST mutant exhibiting a high activity. Specifically, the present invention provides a 2-O-sulfation enzyme mutant, having a substitution of a leucine residue at position 321 with a basic amino acid residue in any one amino acid sequence of: (a) the amino acid sequence of SEQ ID NO: 2; (b) an amino acid sequence comprising one or several amino acid substitutions, deletions, insertions, or additions in the amino acid sequence of SEQ ID NO: 2; (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO: 2; (d) the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; (e) an amino acid sequence comprising one or several amino acid substitutions, deletions, insertions, or additions in the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; (f) an amino acid sequence having 90% or more identity to the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; and having a 2-O-sulfate transfer activity.

    L-GLUTAMATE OXIDASE MUTANT
    8.
    发明公开
    L-GLUTAMATE OXIDASE MUTANT 审中-公开

    公开(公告)号：US20230203456A1

    公开(公告)日：2023-06-29

    申请号：US17947596

    申请日：2022-09-19

    Applicant: AJINOMOTO CO., INC.

    Inventor： Kazutoshi Takahashi ,  Hiroki Yamaguchi ,  Uno Tagami

    IPC: C12N9/06 ,  C12Q1/00 ,  C12P7/50

    CPC classification number: C12N9/0022 ,  C12Y104/03011 ,  C12Q1/005 ,  C12P7/50

    Abstract: The present invention provides an alternative L-glutamate oxidase that allows for measurement of L-glutamate. More specifically, the present invention provides the following L-glutamate oxidase mutant (a) or (b) and the like: (a) an L-glutamate oxidase mutant including an amino acid sequence that has 90% or more identity to an amino acid sequence of SEQ ID NO: 3 and exhibits an activity of oxidizing L-glutamate, except an L-glutamate oxidase including an amino acid sequence of SEQ ID NO: 1; or (b) an L-glutamate oxidase mutant comprising a peptide linker consisting of 1 to 20 amino acid residues which is inserted into one or more sites selected from the group consisting of (1) a site in a region proximity to a boundary between α1 and α2 regions, (2) a site in a region proximity to a boundary between α2 and γ regions and (3) a site in a region proximity to a boundary between γ and β regions in the L-glutamate oxidase mutant (a), and having the activity of oxidizing L-glutamate.

    Modified Leucine Dehydrogenase
    9.
    发明申请
    Modified Leucine Dehydrogenase 有权

    公开(公告)号：US20160222359A1

    公开(公告)日：2016-08-04

    申请号：US15133599

    申请日：2016-04-20

    Applicant: AJINOMOTO CO., INC.

    Inventor： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

    IPC: C12N9/06 ,  C12P13/06 ,  C12Q1/32

    CPC classification number: C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

    Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

Patent Agency Ranking