公开(公告)号：US20210002546A1

公开(公告)日：2021-01-07

申请号：US15999776

申请日：2017-02-14

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun C. YOON ,  Ka Ram KIM ,  Yong Duk HAN ,  Yoo Min PARK ,  Hyeong Jin CHUN

IPC: C09K11/06 ,  C09K11/77 ,  G01N33/533 ,  C01B33/18 ,  C09K11/02

Abstract: Disclosed is a method of manufacturing lanthanide fluorescent silica nanoparticles, including a complex synthesis step of synthesizing a silane-lanthanide chelate complex, an emulsion formation step of forming a water-in-oil microemulsion by dispersing micelles, the water-phase core of which is introduced with the silane-lanthanide chelate complex, in an oil-phase solvent, a silica introduction step of introducing a silica precursor into the microemulsion, and a nanoparticle synthesis step of synthesizing fluorescent silica nanoparticles by crosslinking the silica precursor and the silane-lanthanide chelate complex in the micelles. The lanthanide fluorescent silica nanoparticles thus manufactured can be utilized in fluorescence analysis of inorganic materials or bio-derived materials.

公开(公告)号：US20220334105A1

公开(公告)日：2022-10-20

申请号：US17604010

申请日：2019-11-26

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun Chul YOON ,  Ka Ram KIM ,  Hyeong Jin CHUN ,  Jae Ho KIM

IPC: G01N33/53 ,  G01N15/14 ,  G01N33/58

Abstract: A bio-substance analysis method using a sensing substrate having a fluid channel is disclosed. The method includes mixing retroreflective particles with a detection solution containing a target bio-substance, wherein a first bio-recognition substance selectively reacting with the target bio-substance is modified on the retroreflective particles; placing the sensing substrate so that a bottom is located under a cover in a direction of gravity; injecting the detection solution containing therein the retroreflective particles into a fluid channel and maintaining the solution in the channel for a first time duration; turning the sensing substrate upside down so that the bottom is located above the cover in the direction of gravity and maintaining the sensing substrate in the turned state for a second time duration; irradiating light into the fluid channel through the bottom; and generating and analyzing an image based on light retroreflected from the retroreflective particles.

公开(公告)号：US20210262935A1

公开(公告)日：2021-08-26

申请号：US17173327

申请日：2021-02-11

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun C YOON ,  Ka Ram KIM ,  Hyeong Jin CHUN

IPC: G01N21/64 ,  G02B5/12 ,  C09K11/77 ,  C12Q1/06

Abstract: An optical bio-sensing device includes a transparent substrate covering a top of a space accommodating therein a sample containing a target bio-material; a signal converter fixed to the transparent substrate, and including the upconversion nanoparticles for receiving incident light and emitting converted light of a wavelength shorter than a wavelength of the incident light; a signal reflector including retroreflection particles bindable to the signal converter via the target bio-material, wherein the retroreflection particles retroreflect the converted light; a light source for irradiating the incident light to the signal converter; and a light receiver for receiving light retroreflected from the signal reflector.