公开(公告)号：US20220334105A1

公开(公告)日：2022-10-20

申请号：US17604010

申请日：2019-11-26

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun Chul YOON ,  Ka Ram KIM ,  Hyeong Jin CHUN ,  Jae Ho KIM

IPC: G01N33/53 ,  G01N15/14 ,  G01N33/58

Abstract: A bio-substance analysis method using a sensing substrate having a fluid channel is disclosed. The method includes mixing retroreflective particles with a detection solution containing a target bio-substance, wherein a first bio-recognition substance selectively reacting with the target bio-substance is modified on the retroreflective particles; placing the sensing substrate so that a bottom is located under a cover in a direction of gravity; injecting the detection solution containing therein the retroreflective particles into a fluid channel and maintaining the solution in the channel for a first time duration; turning the sensing substrate upside down so that the bottom is located above the cover in the direction of gravity and maintaining the sensing substrate in the turned state for a second time duration; irradiating light into the fluid channel through the bottom; and generating and analyzing an image based on light retroreflected from the retroreflective particles.

公开(公告)号：US20240255410A1

公开(公告)日：2024-08-01

申请号：US18007348

申请日：2021-07-28

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun Chul YOON ,  Jae-Ho KIM ,  Ka Ram KIM

IPC: G01N15/1434 ,  G01N21/51 ,  G01N33/483

CPC classification number: G01N15/1434 ,  G01N21/51 ,  G01N33/4833

Abstract: The present invention provides a cell measurement device and method in which retroreflective particles are introduced to measure cells, and thus, an optical signal is obtained without fixing of life activity of cells and performing a separate staining process. An example of the use of retroreflection-based cytometry is evaluation of cell migration. The cells capture the retroreflective particles during cell migration or growth, and migrate in a state that the particles are captured in the cell or bind to the cells. The migrated cells are observed in a form of bright dots inside the cells under irradiation of white light as a non-spectroscopic light source to the cells in the retroreflective optical system. The number of bright spots is counted, and thus, the number of cells and the presence thereof thus can be easily measured based on an image.

公开(公告)号：US20240125698A1

公开(公告)日：2024-04-18

申请号：US18276320

申请日：2022-02-10

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun Chul YOON ,  Ka Ram KIM ,  Kyung Wom LEE ,  Jae-ho KIM

IPC: G01N21/55 ,  G01N33/487

CPC classification number: G01N21/55 ,  G01N33/487 ,  G01N2021/177 ,  G01N2021/551

Abstract: A gadget for retroreflected signal measurement is disclosed. The gadget for measuring the retroreflected signal includes a partitioning wall; a portable terminal receiving portion disposed on one surface of the partitioning wall; a biosensor receiving portion disposed on the other surface of the partitioning wall and constructed to receive a biosensor therein; a light exit channel defined in the partitioning wall and at one side thereof; and a light-receiving channel adjacent to the light exit channel and defined in the partitioning wall, wherein light passes through the light exit channel and then is reflected from the biosensor, and then is incident into the light-receiving channel.

公开(公告)号：US20210262935A1

公开(公告)日：2021-08-26

申请号：US17173327

申请日：2021-02-11

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun C YOON ,  Ka Ram KIM ,  Hyeong Jin CHUN

IPC: G01N21/64 ,  G02B5/12 ,  C09K11/77 ,  C12Q1/06

Abstract: An optical bio-sensing device includes a transparent substrate covering a top of a space accommodating therein a sample containing a target bio-material; a signal converter fixed to the transparent substrate, and including the upconversion nanoparticles for receiving incident light and emitting converted light of a wavelength shorter than a wavelength of the incident light; a signal reflector including retroreflection particles bindable to the signal converter via the target bio-material, wherein the retroreflection particles retroreflect the converted light; a light source for irradiating the incident light to the signal converter; and a light receiver for receiving light retroreflected from the signal reflector.

公开(公告)号：US20210002546A1

公开(公告)日：2021-01-07

申请号：US15999776

申请日：2017-02-14

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun C. YOON ,  Ka Ram KIM ,  Yong Duk HAN ,  Yoo Min PARK ,  Hyeong Jin CHUN

IPC: C09K11/06 ,  C09K11/77 ,  G01N33/533 ,  C01B33/18 ,  C09K11/02

Abstract: Disclosed is a method of manufacturing lanthanide fluorescent silica nanoparticles, including a complex synthesis step of synthesizing a silane-lanthanide chelate complex, an emulsion formation step of forming a water-in-oil microemulsion by dispersing micelles, the water-phase core of which is introduced with the silane-lanthanide chelate complex, in an oil-phase solvent, a silica introduction step of introducing a silica precursor into the microemulsion, and a nanoparticle synthesis step of synthesizing fluorescent silica nanoparticles by crosslinking the silica precursor and the silane-lanthanide chelate complex in the micelles. The lanthanide fluorescent silica nanoparticles thus manufactured can be utilized in fluorescence analysis of inorganic materials or bio-derived materials.