公开(公告)号：US11913064B2

公开(公告)日：2024-02-27

申请号：US16498877

申请日：2018-03-09

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun-Chul Yoon ,  Jae-Ho Kim ,  Yong-Duk Han ,  Hyeong-Jin Chun

IPC: G01N21/55 ,  G02B5/126 ,  G02B5/128 ,  G02B5/12 ,  C12Q1/6825

CPC classification number: C12Q1/6825 ,  G01N21/55 ,  G02B5/126 ,  G02B5/128 ,  G01N2021/551 ,  G01N2201/0636 ,  G02B5/12 ,  C12Q1/6825 ,  C12Q2525/301 ,  C12Q2563/131 ,  C12Q2565/519 ,  C12Q2565/60

Abstract: An optical gene biosensor is disclosed. The optical gene biosensor includes a substrate; a molecular beacon anchored to the substrate, wherein the molecular beacon includes an oligonucleotide specifically binding to a target nucleic acid molecule and a first compound bound to a first terminal of the oligonucleotide; an optical marker specifically binding to the first compound, wherein the optical marker is configured to retro-reflect irradiated light; a light source for irradiating the optical marker with light; and a light-receiver for receiving light retro-reflected from the optical marker. The optical gene biosensor may perform accurate quantitative analysis of a target nucleic acid molecule using both non-spectral and spectral light sources.

公开(公告)号：US12117387B2

公开(公告)日：2024-10-15

申请号：US18007348

申请日：2021-07-28

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun Chul Yoon ,  Jae-Ho Kim ,  Ka Ram Kim

IPC: G01N21/47 ,  G01N15/1434 ,  G01N21/51 ,  G01N33/483

CPC classification number: G01N15/1434 ,  G01N21/51 ,  G01N33/4833

Abstract: The present invention provides a cell measurement device and method in which retroreflective particles are introduced to measure cells, and thus, an optical signal is obtained without fixing of life activity of cells and performing a separate staining process. An example of the use of retroreflection-based cytometry is evaluation of cell migration. The cells capture the retroreflective particles during cell migration or growth, and migrate in a state that the particles are captured in the cell or bind to the cells. The migrated cells are observed in a form of bright dots inside the cells under irradiation of white light as a non-spectroscopic light source to the cells in the retroreflective optical system. The number of bright spots is counted, and thus, the number of cells and the presence thereof thus can be easily measured based on an image.