公开(公告)号：US12117387B2

公开(公告)日：2024-10-15

申请号：US18007348

申请日：2021-07-28

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun Chul Yoon ,  Jae-Ho Kim ,  Ka Ram Kim

IPC: G01N21/47 ,  G01N15/1434 ,  G01N21/51 ,  G01N33/483

CPC classification number: G01N15/1434 ,  G01N21/51 ,  G01N33/4833

Abstract: The present invention provides a cell measurement device and method in which retroreflective particles are introduced to measure cells, and thus, an optical signal is obtained without fixing of life activity of cells and performing a separate staining process. An example of the use of retroreflection-based cytometry is evaluation of cell migration. The cells capture the retroreflective particles during cell migration or growth, and migrate in a state that the particles are captured in the cell or bind to the cells. The migrated cells are observed in a form of bright dots inside the cells under irradiation of white light as a non-spectroscopic light source to the cells in the retroreflective optical system. The number of bright spots is counted, and thus, the number of cells and the presence thereof thus can be easily measured based on an image.

公开(公告)号：US12007327B2

公开(公告)日：2024-06-11

申请号：US17173327

申请日：2021-02-11

Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Hyun C Yoon ,  Ka Ram Kim ,  Hyeong Jin Chun

IPC: C09K11/77 ,  C12Q1/06 ,  G01N21/64 ,  G02B5/12

CPC classification number: G01N21/6428 ,  C09K11/7773 ,  C12Q1/06 ,  G01N21/6402 ,  G01N21/6456 ,  G02B5/12 ,  G01N2201/06113

Abstract: An optical bio-sensing device includes a transparent substrate covering a top of a space accommodating therein a sample containing a target bio-material; a signal converter fixed to the transparent substrate, and including the upconversion nanoparticles for receiving incident light and emitting converted light of a wavelength shorter than a wavelength of the incident light; a signal reflector including retroreflection particles bindable to the signal converter via the target bio-material, wherein the retroreflection particles retroreflect the converted light; a light source for irradiating the incident light to the signal converter; and a light receiver for receiving light retroreflected from the signal reflector.