公开(公告)号：US11427627B2

公开(公告)日：2022-08-30

申请号：US14916993

申请日：2014-09-05

Applicant: AMGEN INC.

Inventor： Zhimei Du ,  Pranhitha Reddy ,  Randal B. Bass ,  Feng He ,  William C. Fanslow, III ,  Yuling Zhang ,  Da Ren ,  Randal R. Ketchem

IPC: C07K1/00 ,  C07K16/00 ,  A61K39/395 ,  A61K39/40

Abstract: The present invention relates to variant Fc-containing molecules, such as antibodies and Fc-fusion molecules, having glycosylation characteristics favorable to largescale production of therapeutic molecules containing such variant Fc. Described herein are compositions and methods to improve glycosylation maturation of and to minimize the culture process-dependent effects of Fc-containing molecules, e.g., Fc-fusion molecules and antibodies. Creating single and multiple amino acid substitutions within the Fc domain with the aim to improve high mannose processing and glycosylation maturation.