公开(公告)号：US20240262908A1

公开(公告)日：2024-08-08

申请号：US18429717

申请日：2024-02-01

Applicant: AMGEN INC.

Inventor： Gang Xiao ,  Hyo Seon Chung ,  Quanzhou Luo ,  Tamer Eris ,  Joseph Edward Basconi ,  Xiaoyang Zhao ,  Pavel Bondarenko ,  Krista Marie Petty ,  Oliver Kaltenbrunner

IPC: C07K16/28 ,  C12N5/071 ,  C12N9/64

CPC classification number: C07K16/2803 ,  C12N5/0682 ,  C12N9/641 ,  C07K2317/522 ,  C12N2500/02 ,  C12N2501/999 ,  C12N2523/00 ,  C12Y304/22034

Abstract: Disclosed herein are methods for harvesting a recombinant protein comprising an asparaginyl endopeptidase (AEP) cleavage site, as well as methods for inhibiting cleavage of a recombinant protein comprising an AEP cleavage site, during a biomanufacturing process.