公开(公告)号：US20240262908A1

公开(公告)日：2024-08-08

申请号：US18429717

申请日：2024-02-01

Applicant: AMGEN INC.

Inventor： Gang Xiao ,  Hyo Seon Chung ,  Quanzhou Luo ,  Tamer Eris ,  Joseph Edward Basconi ,  Xiaoyang Zhao ,  Pavel Bondarenko ,  Krista Marie Petty ,  Oliver Kaltenbrunner

IPC: C07K16/28 ,  C12N5/071 ,  C12N9/64

CPC classification number: C07K16/2803 ,  C12N5/0682 ,  C12N9/641 ,  C07K2317/522 ,  C12N2500/02 ,  C12N2501/999 ,  C12N2523/00 ,  C12Y304/22034

Abstract: Disclosed herein are methods for harvesting a recombinant protein comprising an asparaginyl endopeptidase (AEP) cleavage site, as well as methods for inhibiting cleavage of a recombinant protein comprising an AEP cleavage site, during a biomanufacturing process.

公开(公告)号：US11993663B2

公开(公告)日：2024-05-28

申请号：US17346156

申请日：2021-06-11

Applicant: AMGEN INC.

Inventor： Joon Hoi Huh ,  Riki Stevenson ,  Pavel Bondarenko ,  Andrew Nichols ,  Da Ren ,  Neeraj Jagdish Agrawal ,  Richard Smith

IPC: C07K16/40 ,  C07K16/28 ,  G01N11/14

CPC classification number: C07K16/40 ,  C07K16/2869 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2317/94 ,  G01N11/14

Abstract: The present invention concerns a method for preparing antigen binding proteins specific for PCSK9 with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.

公开(公告)号：US20240182558A1

公开(公告)日：2024-06-06

申请号：US18287597

申请日：2022-04-22

Applicant: AMGEN INC.

Inventor： Pavel Bondarenko ,  Liuqing Shi ,  Hao Zhang

IPC: C07K16/24 ,  A61K39/00 ,  C12N15/63

CPC classification number: C07K16/246 ,  C12N15/63 ,  A61K2039/505 ,  A61K2039/545 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/565 ,  C07K2317/92 ,  C07K2317/94

Abstract: The present application relates, in general, to compositions or variants of anti-TSLP antibody tezepelumab having increased stability compared to tezepelumab when stored over long periods of time.

公开(公告)号：US20210395298A1

公开(公告)日：2021-12-23

申请号：US17283135

申请日：2019-10-11

Applicant: AMGEN INC.

Inventor： Pavel Bondarenko ,  Pik Chan ,  Neil Soice

IPC: C07K1/18 ,  C07K16/28

Abstract: The present invention provides an improved manufacturing method for the production of bispecific antibody products, which comprise at least two binding domains. The process comprises at least the method comprising a step of refolding the construct, wherein one or more of the domains comprise a disulfide bond, and wherein the construct is contacted with a refolding buffer to refold the construct to its native form, the buffer comprising (i) a redox and/or oxidizing agent having a concentration of at least 0.1 mM and (ii) a chaotropic agent having a concentration >1 M, and wherein the pH of the refolding buffer corresponds to +/−4.5 of the pI value of the first domain or +/−4.0 of the pI value of the entire construct.

公开(公告)号：US11059908B2

公开(公告)日：2021-07-13

申请号：US16338292

申请日：2017-09-28

Applicant: AMGEN INC.

Inventor： Joon Hoi Huh ,  Riki Stevenson ,  Pavel Bondarenko ,  Andrew Nichols ,  Da Ren ,  Neeraj Jagdish Agrawal

IPC: C07K16/40 ,  C07K16/28 ,  G01N11/14

Abstract: The present invention concerns a method for preparing antigen binding proteins with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.

公开(公告)号：US10513723B2

公开(公告)日：2019-12-24

申请号：US15111470

申请日：2014-12-09

Applicant: AMGEN INC.

Inventor： Sohye Kang ,  Chung Huang, Jr. ,  Hedieh Barkhordarian ,  Pavel Bondarenko ,  Zhongqi Zhang

IPC: C12P21/00 ,  C12N5/071 ,  C07K14/52 ,  C07K16/00 ,  C12N1/38

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

公开(公告)号：US20140371427A1

公开(公告)日：2014-12-18

申请号：US14363735

申请日：2012-12-07

Applicant: AMGEN INC.

Inventor： Thomas M. Dillon ,  Yi-Te Chou ,  Yautyan Chen ,  Pavel Bondarenko ,  Jed J. Wiltzius ,  Diana Woehle

IPC: C07K1/18

CPC classification number: C07K1/18 ,  C07K16/00 ,  C07K16/06 ,  C07K2317/40 ,  C07K2317/52 ,  C07K2317/53

Abstract: Methods for producing an IgG2 antibody preparation enriched for one of several IgG2 structural isoforms, differing by disulfide connectivity in the hinge region of the antibody, are disclosed.

Abstract translation: 公开了用于产生IgG2抗体制剂的方法，所述IgG2抗体制剂富含多种IgG2结构同种型之一，其不同于抗体铰链区中的二硫键连接。

公开(公告)号：US11254963B2

公开(公告)日：2022-02-22

申请号：US16676340

申请日：2019-11-06

Applicant: Amgen Inc.

Inventor： Sohye Kang ,  Chung Huang, Jr. ,  Hedieh Barkhordarian ,  Pavel Bondarenko ,  Zhongqi Zhang

IPC: C12P21/00 ,  C07K16/00 ,  C12N1/38 ,  C07K14/52 ,  C12N5/071

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.