公开(公告)号：US20240262908A1

公开(公告)日：2024-08-08

申请号：US18429717

申请日：2024-02-01

Applicant: AMGEN INC.

Inventor： Gang Xiao ,  Hyo Seon Chung ,  Quanzhou Luo ,  Tamer Eris ,  Joseph Edward Basconi ,  Xiaoyang Zhao ,  Pavel Bondarenko ,  Krista Marie Petty ,  Oliver Kaltenbrunner

IPC: C07K16/28 ,  C12N5/071 ,  C12N9/64

CPC classification number: C07K16/2803 ,  C12N5/0682 ,  C12N9/641 ,  C07K2317/522 ,  C12N2500/02 ,  C12N2501/999 ,  C12N2523/00 ,  C12Y304/22034

Abstract: Disclosed herein are methods for harvesting a recombinant protein comprising an asparaginyl endopeptidase (AEP) cleavage site, as well as methods for inhibiting cleavage of a recombinant protein comprising an AEP cleavage site, during a biomanufacturing process.

公开(公告)号：US20240309044A1

公开(公告)日：2024-09-19

申请号：US18602268

申请日：2024-03-12

Applicant: AMGEN INC.

Inventor： Joseph Edward Basconi ,  Andrew John Maloney ,  Nicholas Anthony Vecchiarello ,  Arun Kannoth Nambiar ,  Glen Bolton

IPC: C07K1/18 ,  B01D15/36 ,  B01D15/38

CPC classification number: C07K1/18 ,  B01D15/363 ,  B01D15/3809

Abstract: Disclosed herein are methods for purifying a recombinant protein from a composition comprising the recombinant protein and at least one impurity, the methods comprising performing anion exchange chromatography (e.g., in flow-through or weak partitioning chromatography mode) using an anion exchange material comprising a primary amine ligand, such as a polyamine ligand.

公开(公告)号：US20240307803A1

公开(公告)日：2024-09-19

申请号：US18602388

申请日：2024-03-12

Applicant: AMGEN INC.

Inventor： Andrew John Maloney ,  David George Meliek ,  Joseph Edward Basconi ,  Sravanthi Vadlamannati ,  Glen Bolton

IPC: B01D36/02 ,  B01D39/16 ,  B01D39/20 ,  B01D69/06 ,  B01D71/68

CPC classification number: B01D36/02 ,  B01D39/16 ,  B01D39/2068 ,  B01D69/06 ,  B01D71/68 ,  B01D2201/182

Abstract: Disclosed herein are methods for removing at least one viral contaminant from a concentrated composition, comprising filtering the concentrated composition through a virus prefilter and a virus filter over one or more filtration cycles, wherein a ratio of the virus prefilter area to the virus filter area in each filtration cycle is at least about 2:1, as well as viral filtration skids for use in such methods.