公开(公告)号：US20240262908A1

公开(公告)日：2024-08-08

申请号：US18429717

申请日：2024-02-01

Applicant: AMGEN INC.

Inventor： Gang Xiao ,  Hyo Seon Chung ,  Quanzhou Luo ,  Tamer Eris ,  Joseph Edward Basconi ,  Xiaoyang Zhao ,  Pavel Bondarenko ,  Krista Marie Petty ,  Oliver Kaltenbrunner

IPC: C07K16/28 ,  C12N5/071 ,  C12N9/64

CPC classification number: C07K16/2803 ,  C12N5/0682 ,  C12N9/641 ,  C07K2317/522 ,  C12N2500/02 ,  C12N2501/999 ,  C12N2523/00 ,  C12Y304/22034

Abstract: Disclosed herein are methods for harvesting a recombinant protein comprising an asparaginyl endopeptidase (AEP) cleavage site, as well as methods for inhibiting cleavage of a recombinant protein comprising an AEP cleavage site, during a biomanufacturing process.

公开(公告)号：US20240076604A1

公开(公告)日：2024-03-07

申请号：US18242116

申请日：2023-09-05

Applicant: AMGEN INC.

Inventor： Gregory S. Stimpfl ,  Oliver Kaltenbrunner ,  Mark O. Brothers ,  David C. Hogenson ,  Thomsen P. Sawicky ,  Min Kyu Choi ,  Jeffrey T. Ranney ,  John C. Roseland

IPC: C12M1/34 ,  C12M1/00

CPC classification number: C12M41/40 ,  C12M23/28 ,  C12M23/40 ,  C12M29/04 ,  C12M29/06 ,  C12M29/10 ,  C12M39/00

Abstract: A large scale bioreactor system includes a stainless steel large scale bioreactor having at least one valve assembly, and an aseptic connector assembly coupled to the at least one valve assembly of the bioreactor. A perfusion device includes an Alternating Tangential Filtration assembly with an autoclaved valve assembly coupled to the aseptic connector assembly, and the aseptic connector assembly includes one of a triclamp aseptic connector or a hose assembly. Single use feed containers include an aseptic connector assembly.