公开(公告)号：US20150184216A1

公开(公告)日：2015-07-02

申请号：US14588790

申请日：2015-01-02

Applicant: AMOREPACIFIC CORPORATION

Inventor： Jun Seong PARK ,  Duck Hee KIM ,  Han Kon KIM ,  Eun Mi KIM ,  Byung Gee KIM

IPC: C12P33/20 ,  C12P17/06

CPC classification number: C12P33/20 ,  C12N1/20 ,  C12N9/0004 ,  C12P17/06 ,  C12P19/02 ,  C12P33/00 ,  C12R1/41 ,  Y02P20/52

Abstract: The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.

Abstract translation: 本发明涉及新根瘤菌属（Rhizobium sp。） GIN611KCTC11708BP或其细胞提取物，对具有糖酵解活性的新型氧化还原酶，与编码该氧化还原酶的基因相连，包含重组载体蛋白的重组菌株或编码重组蛋白的表达载体，以及糖酵解方法 使用与生物催化剂相同的天然产物。 本发明还涉及使用它们的各种天然产物制备糖苷配基的方法。 从本发明的新型微生物分离的新型氧化还原酶不属于葡萄糖苷酶基团，属于氧化还原酶基团，对天然产物具有糖酵解活性。 新型氧化还原酶氧化天然产物的糖苷配基中的糖，从而产生各种糖苷配基。