公开(公告)号：US20210302334A1

公开(公告)日：2021-09-30

申请号：US17220357

申请日：2021-04-01

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexandra ROS ,  Daihyun KIM ,  Austin ECHELMEIER ,  Jorvani CRUZ VILLARREAL ,  Ana EGATZ-GOMEZ ,  Sebastian QUINTANA

IPC: G01N23/20025 ,  B33Y80/00 ,  B01L3/02 ,  B01L3/00

Abstract: Methods and systems are provided for serial femtosecond crystallography for reducing the vast amount of waste of injected crystals practiced with traditional continuous flow injections. A micrometer-scale 3-D printed water-in-oil droplet generator device includes an oil phase inlet channel, an aqueous phase inlet channel, a droplet flow outlet channel, and two embedded non-contact electrodes. The inlet and outlet channels are connected internally at a junction. The electrodes comprise gallium metal injected within the 3-D printed device. Voltage across the electrodes generates water-in-oil droplets, determines a rate for a series of droplets, or triggers a phase shift in the droplets. An external trigger generates the droplets based on the frequency of an XFEL utilized in droplet detection, thereby synchronizing a series of droplets with x-ray pulses for efficient crystal detection. The generated droplets can be coupled to an SFX with XFEL experiment compatible with common liquid injector such as a GDVN.

公开(公告)号：US20240068965A1

公开(公告)日：2024-02-29

申请号：US18504091

申请日：2023-11-07

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexandra ROS ,  Daihyun KIM ,  Austin ECHELMEIER ,  Jorvani CRUZ VILLARREAL ,  Ana EGATZ-GOMEZ ,  Sebastian QUINTANA

IPC: G01N23/20025 ,  B01L3/00 ,  B01L3/02 ,  B33Y80/00

CPC classification number: G01N23/20025 ,  B01L3/0241 ,  B01L3/50273 ,  B33Y80/00 ,  B01L2300/0867 ,  G01N2223/602

Abstract: Methods and systems are provided for serial femtosecond crystallography for reducing the vast amount of waste of injected crystals practiced with traditional continuous flow injections. A micrometer-scale 3-D printed water-in-oil droplet generator device includes an oil phase inlet channel, an aqueous phase inlet channel, a droplet flow outlet channel, and two embedded non-contact electrodes. The inlet and outlet channels are connected internally at a junction. The electrodes comprise gallium metal injected within the 3-D printed device. Voltage across the electrodes generates water-in-oil droplets, determines a rate for a series of droplets, or triggers a phase shift in the droplets. An external trigger generates the droplets based on the frequency of an XFEL utilized in droplet detection, thereby synchronizing a series of droplets with x-ray pulses for efficient crystal detection. The generated droplets can be coupled to an SFX with XFEL experiment compatible with common liquid injector such as a GDVN.

公开(公告)号：US20190178822A1

公开(公告)日：2019-06-13

申请号：US16328262

申请日：2018-05-22

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexandra ROS ,  Gerrit BREHM ,  Garrett NELSON ,  John SPENCE ,  Uwe WEIERSTALL ,  Austin ECHELMEIER

IPC: G01N23/2005 ,  B01F13/00 ,  B33Y80/00 ,  G01N23/20025 ,  B01L3/00

CPC classification number: G01N23/2005 ,  B01F13/0062 ,  B01F2215/0037 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0867 ,  B33Y80/00 ,  G01N23/20025 ,  G01N2223/602 ,  G01N2223/604

Abstract: A 3D printed hybrid nozzle device combining a microfluidic mixer with a liquid jet injector that addresses the bottleneck of investigating substrate-initiated biological reaction paths employing serial crystallography with XFELs. The hybrid nozzle provides for injecting aqueous protein crystal jets after fast mixing (