公开(公告)号：US20230375538A1

公开(公告)日：2023-11-23

申请号：US18017563

申请日：2021-07-22

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joshua LABAER ,  Jin PARK ,  Femina RAUF

IPC: G01N33/537 ,  G01N33/58

CPC classification number: G01N33/537 ,  G01N33/58 ,  G01N2458/10

Abstract: Provided herein are compositions comprising coordinated sets of unique DNA barcodes and methods for using the same for multiplex detection and measurement of multiple target molecules in multiple samples using a single next-generation sequencing reaction. In particular, methods are provided in which unique DNA barcodes linked to affinity reagents are contacted to a sample to bind antigens if present in said sample, and then a PCR-based amplification reaction adds barcoded index sequences that contain universal sequencing adaptors as well as unique barcode sequences and amplifies affinity reagent-bound targets for DNA sequencing.

公开(公告)号：US20180050038A1

公开(公告)日：2018-02-22

申请号：US15555241

申请日：2016-03-02

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Fernanda FESTA ,  Joshua LABAER ,  Jin PARK ,  Femina RAUF

IPC: A61K31/519 ,  A61K31/517

CPC classification number: A61K31/519 ,  A61K31/517

Abstract: Disclosed herein are methods of treating diseases that exhibit over-activation of the ERBB4 pathway, such as breast cancer and lung cancer. The method comprises administering to a subject having a disease that exhibits over-activation of the ERBB4 pathway and a gene expression profile consistent with an ERBB4-sensitive profile a therapeutically effective amount of an ERBB4 inhibitor, wherein the disease is effectively treated. Gene expression profiles of ERBB4-sensitive cells and microarrays suitable for protein-tyrosine kinases are also provided.

公开(公告)号：US20210163926A1

公开(公告)日：2021-06-03

申请号：US16954201

申请日：2019-01-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joshua LABAER ,  Jin PARK

IPC: C12N15/10

Abstract: Disclosed is a method of functional genomics determination including transducing a cell population with a set of nucleic acid molecules including a pooled library of genomic perturbagens to integrate multiple perturbagen cassettes into the genome. A phenotype of individual cells is determined and single cells of the population with targeted phenotypes are individually sorted into a set of compartments. Each compartment includes a forward primer with a nucleic acid sequence (NAS) that specifically binds a common nucleic acid sequence on the nucleic acid molecules and a compartment (cell)-specific nucleic acid barcode. Also included is a reverse primer with a NAS that specifically binds a common NAS on the nucleic acid molecules comprising a pooled library of genomic perturbagens. The genome-integrated perturbagen cassettes are create amplicons which are pooled and sequences determined. This method can be applied to other genome-level single-cell applications—immune receptor profiling, targeted DNA/RNA sequencing, and metagenomics.

公开(公告)号：US20230384306A1

公开(公告)日：2023-11-30

申请号：US18326837

申请日：2023-05-31

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Mahasish SHOME ,  Yunro CHUNG ,  Ramani CHAVAN ,  Jin PARK ,  Ji QIU ,  Joshua LABAER

IPC: G01N33/564 ,  G01N33/569 ,  G01N33/574

CPC classification number: G01N33/564 ,  G01N33/56983 ,  G01N33/57484

Abstract: The present invention relates autoantibody biomarkers, kits, and methods for increasing the accuracy of a cancer or autoimmune screening of a subject suspected of having cancer or an autoimmune condition.